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Frequencies of the precision ID ancestry panel markers in Basques using the Ion Torrent PGMTM platform.
Forensic Sci Int Genet. 2017 11; 31:e1-e4.FS

Abstract

The Precision ID Ancestry Panel for the Ion Torrent PGM™ platform is able to genotype 165 autosomal SNPs by massively parallel sequencing (MPS). In the present study, we evaluated the depth of coverage, the locus strand balance and the heterozygote balance of the above panel. The SNPs rs1569175, rs2306040, rs9845457, rs1407434, and rs10007810 showed a low performance, due either to a low coverage, locus strand imbalance or heterozygote imbalance. To further to assess this panel, we analyzed 108 Basques. All loci proved to be in Hardy-Weinberg equilibrium and we did not detect any association between them with the exception of the pair rs3916235-rs4891825 in chromosome 18. The forensic parameters combined match probability and combined power of exclusion were estimated to be 3.13×10-35 and 99.9972%, respectively. In conclusion, the Precision ID Ancestry panel along with this new MPS technology constitute a very promising tool for human identification and biogeographical ancestry inference in routine casework in the forensic field.

Authors+Show Affiliations

Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain. Electronic address: gobies01@euskalnet.net.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.Forensic Science Unit, Forensic Genetics Section, Basque Country Police, Erandio, Bizkaia, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28935228

Citation

García, O, et al. "Frequencies of the Precision ID Ancestry Panel Markers in Basques Using the Ion Torrent PGMTM Platform." Forensic Science International. Genetics, vol. 31, 2017, pp. e1-e4.
García O, Ajuriagerra JA, Alday A, et al. Frequencies of the precision ID ancestry panel markers in Basques using the Ion Torrent PGMTM platform. Forensic Sci Int Genet. 2017;31:e1-e4.
García, O., Ajuriagerra, J. A., Alday, A., Alonso, S., Pérez, J. A., Soto, A., Uriarte, I., & Yurrebaso, I. (2017). Frequencies of the precision ID ancestry panel markers in Basques using the Ion Torrent PGMTM platform. Forensic Science International. Genetics, 31, e1-e4. https://doi.org/10.1016/j.fsigen.2017.09.006
García O, et al. Frequencies of the Precision ID Ancestry Panel Markers in Basques Using the Ion Torrent PGMTM Platform. Forensic Sci Int Genet. 2017;31:e1-e4. PubMed PMID: 28935228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequencies of the precision ID ancestry panel markers in Basques using the Ion Torrent PGMTM platform. AU - García,O, AU - Ajuriagerra,J A, AU - Alday,A, AU - Alonso,S, AU - Pérez,J A, AU - Soto,A, AU - Uriarte,I, AU - Yurrebaso,I, Y1 - 2017/09/12/ PY - 2017/06/22/received PY - 2017/09/03/revised PY - 2017/09/08/accepted PY - 2017/9/25/pubmed PY - 2017/12/12/medline PY - 2017/9/23/entrez KW - Basques KW - Ion Torrent PGM(TM) KW - Massively parallel sequencing (MPS) KW - Population genetics KW - Precision ID Ancestry Panel SP - e1 EP - e4 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 31 N2 - The Precision ID Ancestry Panel for the Ion Torrent PGM™ platform is able to genotype 165 autosomal SNPs by massively parallel sequencing (MPS). In the present study, we evaluated the depth of coverage, the locus strand balance and the heterozygote balance of the above panel. The SNPs rs1569175, rs2306040, rs9845457, rs1407434, and rs10007810 showed a low performance, due either to a low coverage, locus strand imbalance or heterozygote imbalance. To further to assess this panel, we analyzed 108 Basques. All loci proved to be in Hardy-Weinberg equilibrium and we did not detect any association between them with the exception of the pair rs3916235-rs4891825 in chromosome 18. The forensic parameters combined match probability and combined power of exclusion were estimated to be 3.13×10-35 and 99.9972%, respectively. In conclusion, the Precision ID Ancestry panel along with this new MPS technology constitute a very promising tool for human identification and biogeographical ancestry inference in routine casework in the forensic field. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/28935228/Frequencies_of_the_precision_ID_ancestry_panel_markers_in_Basques_using_the_Ion_Torrent_PGM[TM]_platform_ DB - PRIME DP - Unbound Medicine ER -