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Massively parallel sequencing of 32 forensic markers using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System.
Forensic Sci Int Genet. 2017 11; 31:126-134.FS

Abstract

Massively parallel sequencing (MPS) technologies have proved capable of sequencing the majority of the key forensic STR markers. By MPS, not only the repeat-length size but also sequence variations could be detected. Recently, Thermo Fisher Scientific has designed an advanced MPS 32-plex panel, named the Precision ID GlobalFiler™ NGS STR Panel, where the primer set has been designed specifically for the purpose of MPS technologies and the data analysis are supported by a new version HID STR Genotyper Plugin (V4.0). In this study, a series of experiments that evaluated concordance, reliability, sensitivity of detection, mixture analysis, and the ability to analyze case-type and challenged samples were conducted. In addition, 106 unrelated Han individuals were sequenced to perform genetic analyses of allelic diversity. As expected, MPS detected broader allele variations and gained higher power of discrimination and exclusion rate. MPS results were found to be concordant with current capillary electrophoresis methods, and single source complete profiles could be obtained stably using as little as 100pg of input DNA. Moreover, this MPS panel could be adapted to case-type samples and partial STR genotypes of the minor contributor could be detected up to 19:1 mixture. Aforementioned results indicate that the Precision ID GlobalFiler™ NGS STR Panel is reliable, robust and reproducible and have the potential to be used as a tool for human forensics.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Z
Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, China. Electronic address: wangzhengtim@scu.edu.cn.
Zhou D
Thermo Fisher Scientific, Shanghai 200050, China.
Wang H
Department of Forensic Genetics, Institute of Forensic Science, Chengdu Public Security Bureau, Chengdu 610081, China.
Jia Z
Department of Criminal Science and Technology, People's Public Security University of China, Beijing 100038, China.
Liu J
Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Qian X
Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li C
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, China.
Hou Y
Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, China; Collaborative Innovation Center of Judicial Civilization, Beijing 100088, China. Electronic address: forensic@scu.edu.cn.

MeSH

DNA FingerprintingEthnicityFemaleGenetic MarkersHigh-Throughput Nucleotide SequencingHumansMaleMicrosatellite RepeatsSequence Analysis, DNA

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28938153

Citation

Wang, Zheng, et al. "Massively Parallel Sequencing of 32 Forensic Markers Using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System." Forensic Science International. Genetics, vol. 31, 2017, pp. 126-134.
Wang Z, Zhou D, Wang H, et al. Massively parallel sequencing of 32 forensic markers using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System. Forensic Sci Int Genet. 2017;31:126-134.
Wang, Z., Zhou, D., Wang, H., Jia, Z., Liu, J., Qian, X., Li, C., & Hou, Y. (2017). Massively parallel sequencing of 32 forensic markers using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System. Forensic Science International. Genetics, 31, 126-134. https://doi.org/10.1016/j.fsigen.2017.09.004
Wang Z, et al. Massively Parallel Sequencing of 32 Forensic Markers Using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System. Forensic Sci Int Genet. 2017;31:126-134. PubMed PMID: 28938153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Massively parallel sequencing of 32 forensic markers using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System. AU - Wang,Zheng, AU - Zhou,Di, AU - Wang,Hui, AU - Jia,Zhenjun, AU - Liu,Jing, AU - Qian,Xiaoqin, AU - Li,Chengtao, AU - Hou,Yiping, Y1 - 2017/09/08/ PY - 2017/02/07/received PY - 2017/08/26/revised PY - 2017/09/06/accepted PY - 2017/9/25/pubmed PY - 2017/12/12/medline PY - 2017/9/23/entrez KW - Ion PGM™ System KW - Massively parallel sequencing (MPS) KW - Precision ID GlobalFiler™ NGS STR Panel KW - Short tandem repeat (STR) SP - 126 EP - 134 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 31 N2 - Massively parallel sequencing (MPS) technologies have proved capable of sequencing the majority of the key forensic STR markers. By MPS, not only the repeat-length size but also sequence variations could be detected. Recently, Thermo Fisher Scientific has designed an advanced MPS 32-plex panel, named the Precision ID GlobalFiler™ NGS STR Panel, where the primer set has been designed specifically for the purpose of MPS technologies and the data analysis are supported by a new version HID STR Genotyper Plugin (V4.0). In this study, a series of experiments that evaluated concordance, reliability, sensitivity of detection, mixture analysis, and the ability to analyze case-type and challenged samples were conducted. In addition, 106 unrelated Han individuals were sequenced to perform genetic analyses of allelic diversity. As expected, MPS detected broader allele variations and gained higher power of discrimination and exclusion rate. MPS results were found to be concordant with current capillary electrophoresis methods, and single source complete profiles could be obtained stably using as little as 100pg of input DNA. Moreover, this MPS panel could be adapted to case-type samples and partial STR genotypes of the minor contributor could be detected up to 19:1 mixture. Aforementioned results indicate that the Precision ID GlobalFiler™ NGS STR Panel is reliable, robust and reproducible and have the potential to be used as a tool for human forensics. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/28938153/Massively_parallel_sequencing_of_32_forensic_markers_using_the_Precision_ID_GlobalFiler���_NGS_STR_Panel_and_the_Ion_PGM���_System_ DB - PRIME DP - Unbound Medicine ER -