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Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein.
Oncotarget. 2017 Aug 29; 8(35):58865-58871.O

Abstract

CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and various other tumors in meat and egg type chickens. Here, we identified a chicken ZAP (chZAP) that increased at early stage, and subsequently decreased after infection of ALV-J in DF-1 cells, indicating the inducible feature of the endogenous chZAP. To demonstrate the inhibitory effect on ALV-J replication by chZAP, we expressed exogenous chZAP by lentivirus based vectors in DF-1 cells that infected by ALV-J. The result showed that overexpression of chZAP significantly inhibited ALV-J replication at both mRNA level and protein level. Consequently, knockdown of endogenous chZAP by RNAi facilitated ALV-J replication in DF-1 cells. Further, we demonstrated that chZAP interacts with SU protein (encode by gp85 gene) of ALV-J in cytoplasm. Taken together, our results demonstrated that chZAP inhibits ALV-J by both mRNA and protein pathway and it may shed light on a novel antiviral approach in poultry.

Authors+Show Affiliations

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28938603

Citation

Zhu, Mingjun, et al. "Inhibition of Avian Tumor Virus Replication By CCCH-type Zinc Finger Antiviral Protein." Oncotarget, vol. 8, no. 35, 2017, pp. 58865-58871.
Zhu M, Ma X, Cui X, et al. Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein. Oncotarget. 2017;8(35):58865-58871.
Zhu, M., Ma, X., Cui, X., Zhou, J., Li, C., Huang, L., Shang, Y., & Cheng, Z. (2017). Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein. Oncotarget, 8(35), 58865-58871. https://doi.org/10.18632/oncotarget.19378
Zhu M, et al. Inhibition of Avian Tumor Virus Replication By CCCH-type Zinc Finger Antiviral Protein. Oncotarget. 2017 Aug 29;8(35):58865-58871. PubMed PMID: 28938603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of avian tumor virus replication by CCCH-type zinc finger antiviral protein. AU - Zhu,Mingjun, AU - Ma,Xiaoqian, AU - Cui,Xiyao, AU - Zhou,Jing, AU - Li,Chengui, AU - Huang,Libo, AU - Shang,Yingli, AU - Cheng,Ziqiang, Y1 - 2017/07/19/ PY - 2017/02/18/received PY - 2017/07/12/accepted PY - 2017/9/24/entrez PY - 2017/9/25/pubmed PY - 2017/9/25/medline KW - RNAi KW - avian leukosis virus subgroup J KW - chicken CCCH type zinc finger antiviral protein KW - overexpression SP - 58865 EP - 58871 JF - Oncotarget JO - Oncotarget VL - 8 IS - 35 N2 - CCCH type zinc finger antiviral protein (ZAP) is a host restriction factor that inhibits the replication of a variety of viruses in mammals. However, little is known about its antiviral activity on avian tumor virus. Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and various other tumors in meat and egg type chickens. Here, we identified a chicken ZAP (chZAP) that increased at early stage, and subsequently decreased after infection of ALV-J in DF-1 cells, indicating the inducible feature of the endogenous chZAP. To demonstrate the inhibitory effect on ALV-J replication by chZAP, we expressed exogenous chZAP by lentivirus based vectors in DF-1 cells that infected by ALV-J. The result showed that overexpression of chZAP significantly inhibited ALV-J replication at both mRNA level and protein level. Consequently, knockdown of endogenous chZAP by RNAi facilitated ALV-J replication in DF-1 cells. Further, we demonstrated that chZAP interacts with SU protein (encode by gp85 gene) of ALV-J in cytoplasm. Taken together, our results demonstrated that chZAP inhibits ALV-J by both mRNA and protein pathway and it may shed light on a novel antiviral approach in poultry. SN - 1949-2553 UR - https://www.unboundmedicine.com/medline/citation/28938603/Inhibition_of_avian_tumor_virus_replication_by_CCCH_type_zinc_finger_antiviral_protein_ L2 - https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.19378 DB - PRIME DP - Unbound Medicine ER -
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