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Cloning and functional characterization of fads2 desaturase and elovl5 elongase from Japanese flounder Paralichthys olivaceus.

Abstract

Japanese flounder Paralichthys olivaceus has an essential requirement for long-chain polyunsaturated fatty acids (LC-PUFA), particularly docosahexaenoic acid and eicosapentaenoic acid, but the enzymes involved in LC-PUFA biosynthesis are thought to be absent or to have low activity. Teleost fish, in particular, have quite diversified substrate preference of these enzymes even among closely related species, implying that each species could have different LC-PUFA biosynthetic capabilities. Therefore, in the present study, we characterized Japanese flounder fatty acid desaturase 2 (Fads2) and elongation of very long-chain fatty acids protein 5 (Elovl5) in order to precisely characterize the LC-PUFA biosynthesis pathway. Fads2 has Δ6 and Δ8 desaturase activity and Elovl5 has elongase activity toward C18 and C20 PUFA, suggesting that Japanese flounder is capable of synthesizing 20:4n-3 and 20:3n-6 from 18:3n-3 and 18:2n-6, respectively. Expression analysis showed that the fads2 was highly expressed in the brain and eye, while the elovl5 was highly expressed in the eye and pyloric caeca. This information will be beneficial for developing an ideal feed to support the aquaculture of Japanese flounder.

Authors+Show Affiliations

Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan. Electronic address: goro@kaiyodai.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28943298

Citation

Kabeya, Naoki, et al. "Cloning and Functional Characterization of Fads2 Desaturase and Elovl5 Elongase From Japanese Flounder Paralichthys Olivaceus." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 214, 2017, pp. 36-46.
Kabeya N, Chiba M, Haga Y, et al. Cloning and functional characterization of fads2 desaturase and elovl5 elongase from Japanese flounder Paralichthys olivaceus. Comp Biochem Physiol B, Biochem Mol Biol. 2017;214:36-46.
Kabeya, N., Chiba, M., Haga, Y., Satoh, S., & Yoshizaki, G. (2017). Cloning and functional characterization of fads2 desaturase and elovl5 elongase from Japanese flounder Paralichthys olivaceus. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 214, pp. 36-46. doi:10.1016/j.cbpb.2017.09.002.
Kabeya N, et al. Cloning and Functional Characterization of Fads2 Desaturase and Elovl5 Elongase From Japanese Flounder Paralichthys Olivaceus. Comp Biochem Physiol B, Biochem Mol Biol. 2017;214:36-46. PubMed PMID: 28943298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and functional characterization of fads2 desaturase and elovl5 elongase from Japanese flounder Paralichthys olivaceus. AU - Kabeya,Naoki, AU - Chiba,Mizumo, AU - Haga,Yutaka, AU - Satoh,Shuichi, AU - Yoshizaki,Goro, Y1 - 2017/09/22/ PY - 2017/07/11/received PY - 2017/09/13/revised PY - 2017/09/15/accepted PY - 2017/9/26/pubmed PY - 2017/10/31/medline PY - 2017/9/26/entrez KW - Biosynthesis KW - Desaturase·docosahexaenoic acid KW - Eicosapentaenoic acid KW - Elongase KW - Japanese flounder KW - Polyunsaturated fatty acids SP - 36 EP - 46 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 214 N2 - Japanese flounder Paralichthys olivaceus has an essential requirement for long-chain polyunsaturated fatty acids (LC-PUFA), particularly docosahexaenoic acid and eicosapentaenoic acid, but the enzymes involved in LC-PUFA biosynthesis are thought to be absent or to have low activity. Teleost fish, in particular, have quite diversified substrate preference of these enzymes even among closely related species, implying that each species could have different LC-PUFA biosynthetic capabilities. Therefore, in the present study, we characterized Japanese flounder fatty acid desaturase 2 (Fads2) and elongation of very long-chain fatty acids protein 5 (Elovl5) in order to precisely characterize the LC-PUFA biosynthesis pathway. Fads2 has Δ6 and Δ8 desaturase activity and Elovl5 has elongase activity toward C18 and C20 PUFA, suggesting that Japanese flounder is capable of synthesizing 20:4n-3 and 20:3n-6 from 18:3n-3 and 18:2n-6, respectively. Expression analysis showed that the fads2 was highly expressed in the brain and eye, while the elovl5 was highly expressed in the eye and pyloric caeca. This information will be beneficial for developing an ideal feed to support the aquaculture of Japanese flounder. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/28943298/Cloning_and_functional_characterization_of_fads2_desaturase_and_elovl5_elongase_from_Japanese_flounder_Paralichthys_olivaceus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(17)30140-9 DB - PRIME DP - Unbound Medicine ER -