Tags

Type your tag names separated by a space and hit enter

Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol.
Rev Argent Microbiol. 2018 Jan - Mar; 50(1):70-74.RA

Abstract

The aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against Fusarium verticillioides and Rhizopus stolonifer, and to obtain primary growth models. Minimal inhibitory concentration (MIC) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). Mixtures were evaluated using concentrations below MIC values. Radial growth curves were described by the modified Gompertz equation. MIC values of carvacrol were 200mg/l for both fungi. Meanwhile, MIC values of thymol were between 500 and 400mg/l for F. verticillioides and R. stolonifer, respectively. A synergistic effect below MIC concentrations for carvacrol (100mg/l) and thymol (100-375mg/l) was observed. Significant differences (p<0.05) between the Gompertz parameters for the antimicrobial concentrations and their tested mixtures established an inverse relationship between antimicrobial concentration and mycelial development of both fungi. Modified Gompertz parameters can be useful to determine fungistatic concentrations.

Authors+Show Affiliations

Departamento de Bioquímica-Alimentos, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Edificio 105E, 14 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72420 Puebla, Puebla, Mexico.Departamento de Bioquímica-Alimentos, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Edificio 105E, 14 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72420 Puebla, Puebla, Mexico.Departamento de Bioquímica-Alimentos, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Edificio 105E, 14 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72420 Puebla, Puebla, Mexico.Departamento de Ingeniería Química, Alimentos y Ambiental, Universidad de las Américas Puebla, Cholula, Pue. 72810, Mexico.Departamento de Bioquímica-Alimentos, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Edificio 105E, 14 Sur y Av. San Claudio, Ciudad Universitaria, Col. San Manuel, 72420 Puebla, Puebla, Mexico. Electronic address: raul.avila@correo.buap.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28947087

Citation

Ochoa-Velasco, Carlos E., et al. "Growth Modeling to Control (in Vitro) Fusarium Verticillioides and Rhizopus Stolonifer With Thymol and Carvacrol." Revista Argentina De Microbiologia, vol. 50, no. 1, 2018, pp. 70-74.
Ochoa-Velasco CE, Navarro-Cruz AR, Vera-López O, et al. Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol. Rev Argent Microbiol. 2018;50(1):70-74.
Ochoa-Velasco, C. E., Navarro-Cruz, A. R., Vera-López, O., Palou, E., & Avila-Sosa, R. (2018). Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol. Revista Argentina De Microbiologia, 50(1), 70-74. https://doi.org/10.1016/j.ram.2016.11.010
Ochoa-Velasco CE, et al. Growth Modeling to Control (in Vitro) Fusarium Verticillioides and Rhizopus Stolonifer With Thymol and Carvacrol. Rev Argent Microbiol. 2018 Jan - Mar;50(1):70-74. PubMed PMID: 28947087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol. AU - Ochoa-Velasco,Carlos E, AU - Navarro-Cruz,Addí R, AU - Vera-López,Obdulia, AU - Palou,Enrique, AU - Avila-Sosa,Raul, Y1 - 2017/09/23/ PY - 2016/02/25/received PY - 2016/11/07/revised PY - 2016/11/19/accepted PY - 2017/9/28/pubmed PY - 2019/2/12/medline PY - 2017/9/27/entrez KW - Carvacrol KW - Crecimiento fúngico KW - Fungal growth KW - Sinergismo KW - Synergism KW - Thymol KW - Timol SP - 70 EP - 74 JF - Revista Argentina de microbiologia JO - Rev. Argent. Microbiol. VL - 50 IS - 1 N2 - The aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against Fusarium verticillioides and Rhizopus stolonifer, and to obtain primary growth models. Minimal inhibitory concentration (MIC) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). Mixtures were evaluated using concentrations below MIC values. Radial growth curves were described by the modified Gompertz equation. MIC values of carvacrol were 200mg/l for both fungi. Meanwhile, MIC values of thymol were between 500 and 400mg/l for F. verticillioides and R. stolonifer, respectively. A synergistic effect below MIC concentrations for carvacrol (100mg/l) and thymol (100-375mg/l) was observed. Significant differences (p<0.05) between the Gompertz parameters for the antimicrobial concentrations and their tested mixtures established an inverse relationship between antimicrobial concentration and mycelial development of both fungi. Modified Gompertz parameters can be useful to determine fungistatic concentrations. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/28947087/Growth_modeling_to_control__in_vitro__Fusarium_verticillioides_and_Rhizopus_stolonifer_with_thymol_and_carvacrol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0325-7541(17)30100-1 DB - PRIME DP - Unbound Medicine ER -