Tags

Type your tag names separated by a space and hit enter

Advantages of a validated UPLC-MS/MS standard addition method for the quantification of A-type dimeric and trimeric proanthocyanidins in cranberry extracts in comparison with well-known quantification methods.
J Pharm Biomed Anal 2018; 148:32-41JP

Abstract

The berries of Vaccinium macrocarpon, cranberry, are widely used for the prevention of urinary tract infections. This species contains A-type proanthocyanidins (PACs), which intervene in the initial phase of the development of urinary tract infections by preventing the adherence of Escherichia coli by their P-type fimbriae to uroepithelial cells. Unfortunately, the existing clinical studies used different cranberry preparations, which were poorly standardized. Because of this, the results were hard to compare, which led sometimes to conflicting results. Currently, PACs are quantified using the rather non-specific spectrophotometric 4-dimethylaminocinnamaldehyde (DMAC) method. In addition, a normal phase HPTLC-densitometric method, a HPLC-UV method and three LC-MS/MS methods for quantification of procyanidin A2 were recently published. All these methods contain some shortcomings and errors. Hence, the development and validation of a fast and sensitive standard addition LC-MS/MS method for the simultaneous quantification of A-type dimers and trimers in a cranberry dry extract was carried out. A linear calibration model could be adopted for dimers and, after logaritmic transformation, for trimers. The maximal interday and interconcentration precision was found to be 4.86% and 4.28% for procyanidin A2, and 5.61% and 7.65% for trimeric PACs, which are all acceptable values for an analytical method using LC-MS/MS. In addition, twelve different cranberry extracts were analyzed by means of the newly validated method and other widely used methods. There appeared to be an enormous variation in dimeric and trimeric PAC content. Comparison of these results with LC-MS/MS analysis without standard addition showed the presence of matrix effects for some of the extracts and proved the necessity of standard addition. A comparison of the well-known and widely used DMAC method, the butanol-HCl assay and this newly developed LC-MS/MS method clearly indicated the need for a reliable method able to quantify A-type PACs, which are considered to be the pharmacologically active constituents of cranberry, since neither the DMAC or butanol-HCl assays are capable of distinguishing between A and B-type PACs and therefore cannot detect adulterations with, for example, extracts with a high B-type PAC content. Hence, the combination of the DMAC method or butanol-HCl assay with this more specific LC-MS/MS assay could overcome these shortcomings.

Authors+Show Affiliations

Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Electronic address: ines_van_dooren@hotmail.com.Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.Natural Products & Food Research and Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28950214

Citation

van Dooren, Ines, et al. "Advantages of a Validated UPLC-MS/MS Standard Addition Method for the Quantification of A-type Dimeric and Trimeric Proanthocyanidins in Cranberry Extracts in Comparison With Well-known Quantification Methods." Journal of Pharmaceutical and Biomedical Analysis, vol. 148, 2018, pp. 32-41.
van Dooren I, Foubert K, Theunis M, et al. Advantages of a validated UPLC-MS/MS standard addition method for the quantification of A-type dimeric and trimeric proanthocyanidins in cranberry extracts in comparison with well-known quantification methods. J Pharm Biomed Anal. 2018;148:32-41.
van Dooren, I., Foubert, K., Theunis, M., Naessens, T., Pieters, L., & Apers, S. (2018). Advantages of a validated UPLC-MS/MS standard addition method for the quantification of A-type dimeric and trimeric proanthocyanidins in cranberry extracts in comparison with well-known quantification methods. Journal of Pharmaceutical and Biomedical Analysis, 148, pp. 32-41. doi:10.1016/j.jpba.2017.09.002.
van Dooren I, et al. Advantages of a Validated UPLC-MS/MS Standard Addition Method for the Quantification of A-type Dimeric and Trimeric Proanthocyanidins in Cranberry Extracts in Comparison With Well-known Quantification Methods. J Pharm Biomed Anal. 2018 Jan 30;148:32-41. PubMed PMID: 28950214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Advantages of a validated UPLC-MS/MS standard addition method for the quantification of A-type dimeric and trimeric proanthocyanidins in cranberry extracts in comparison with well-known quantification methods. AU - van Dooren,Ines, AU - Foubert,Kenn, AU - Theunis,Mart, AU - Naessens,Tania, AU - Pieters,Luc, AU - Apers,Sandra, Y1 - 2017/09/09/ PY - 2017/03/17/received PY - 2017/09/01/revised PY - 2017/09/03/accepted PY - 2017/9/28/pubmed PY - 2018/7/4/medline PY - 2017/9/27/entrez KW - A-type procyanidins KW - Cranberry KW - LC–MS/MS KW - Quality control KW - Urinary tract infections KW - Vaccinium macrocarpon SP - 32 EP - 41 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 148 N2 - The berries of Vaccinium macrocarpon, cranberry, are widely used for the prevention of urinary tract infections. This species contains A-type proanthocyanidins (PACs), which intervene in the initial phase of the development of urinary tract infections by preventing the adherence of Escherichia coli by their P-type fimbriae to uroepithelial cells. Unfortunately, the existing clinical studies used different cranberry preparations, which were poorly standardized. Because of this, the results were hard to compare, which led sometimes to conflicting results. Currently, PACs are quantified using the rather non-specific spectrophotometric 4-dimethylaminocinnamaldehyde (DMAC) method. In addition, a normal phase HPTLC-densitometric method, a HPLC-UV method and three LC-MS/MS methods for quantification of procyanidin A2 were recently published. All these methods contain some shortcomings and errors. Hence, the development and validation of a fast and sensitive standard addition LC-MS/MS method for the simultaneous quantification of A-type dimers and trimers in a cranberry dry extract was carried out. A linear calibration model could be adopted for dimers and, after logaritmic transformation, for trimers. The maximal interday and interconcentration precision was found to be 4.86% and 4.28% for procyanidin A2, and 5.61% and 7.65% for trimeric PACs, which are all acceptable values for an analytical method using LC-MS/MS. In addition, twelve different cranberry extracts were analyzed by means of the newly validated method and other widely used methods. There appeared to be an enormous variation in dimeric and trimeric PAC content. Comparison of these results with LC-MS/MS analysis without standard addition showed the presence of matrix effects for some of the extracts and proved the necessity of standard addition. A comparison of the well-known and widely used DMAC method, the butanol-HCl assay and this newly developed LC-MS/MS method clearly indicated the need for a reliable method able to quantify A-type PACs, which are considered to be the pharmacologically active constituents of cranberry, since neither the DMAC or butanol-HCl assays are capable of distinguishing between A and B-type PACs and therefore cannot detect adulterations with, for example, extracts with a high B-type PAC content. Hence, the combination of the DMAC method or butanol-HCl assay with this more specific LC-MS/MS assay could overcome these shortcomings. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/28950214/Advantages_of_a_validated_UPLC_MS/MS_standard_addition_method_for_the_quantification_of_A_type_dimeric_and_trimeric_proanthocyanidins_in_cranberry_extracts_in_comparison_with_well_known_quantification_methods_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)30674-X DB - PRIME DP - Unbound Medicine ER -