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Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test.
Braz J Biol. 2018 May; 78(2):345-350.BJ

Abstract

Salacia crassifolia (Mart. Ex. Schult.) G. Don. is a bush which belongs to Celastraceae family and occurs specially in Brazilian Cerrado. Its leaves, stem, seeds and fruits are popularly used for several medicinal purposes, such as antitumoral, antirheumatic, anti-inflammatory and antimicrobial. In this study, the mutagenic and antimutagenic activities of S. crassifolia stem bark fractions (hexane, ethyl acetate and hydroalcoholic) were evaluated by the Ames mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. By the obtained results, all S. crassifolia fractions did not significantly increase the number of prototrophic revertants for histidine (His+) in both S. typhimurium strains tested (p > 0.05), suggesting absence of mutagenicity. Regarding antimutagenicity, the fractions ethyl acetate and hydroalcoholic significantly decreased the number of His+ revertants colonies induced by positive control for strain TA98 (p < 0.05), demonstrating protection against mutagenicity induced by 4-nitroquinolile1-oxide, whereas the hexane fraction did not show antimutagenic effect in this strain. In the TA100 strain, all fractions of S. crassifolia protected DNA against the harmful action of sodium azide, and the hexane fraction exhibited the greatest protection in this work. Thus, it's possible conclude that the fractions of S. crassifolia tested in this study could be used in chemoprevention.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Carneiro CC
Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Véras JH
Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Góes BRL
Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Pérez CN
Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Chen-Chen L
Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.

MeSH

4-Nitroquinoline-1-oxideAntimutagenic AgentsMutagenicity TestsMutagensPlant ExtractsSalaciaSalmonella typhimurium

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28954013

Citation

Carneiro, C C., et al. "Mutagenicity and Antimutagenicity of Salacia Crassifolia (mart. Ex. Schult.) G. Don. Evaluated By Ames Test." Brazilian Journal of Biology = Revista Brasleira De Biologia, vol. 78, no. 2, 2018, pp. 345-350.
Carneiro CC, Véras JH, Góes BRL, et al. Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test. Braz J Biol. 2018;78(2):345-350.
Carneiro, C. C., Véras, J. H., Góes, B. R. L., Pérez, C. N., & Chen-Chen, L. (2018). Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test. Brazilian Journal of Biology = Revista Brasleira De Biologia, 78(2), 345-350. https://doi.org/10.1590/1519-6984.166593
Carneiro CC, et al. Mutagenicity and Antimutagenicity of Salacia Crassifolia (mart. Ex. Schult.) G. Don. Evaluated By Ames Test. Braz J Biol. 2018;78(2):345-350. PubMed PMID: 28954013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity and antimutagenicity of Salacia crassifolia (mart. Ex. Schult.) G. Don. evaluated by Ames test. AU - Carneiro,C C, AU - Véras,J Holanda, AU - Góes,B R Lima, AU - Pérez,C N, AU - Chen-Chen,L, Y1 - 2017/09/21/ PY - 2016/07/13/received PY - 2016/11/08/accepted PY - 2017/9/28/pubmed PY - 2018/9/19/medline PY - 2017/9/28/entrez SP - 345 EP - 350 JF - Brazilian journal of biology = Revista brasleira de biologia JO - Braz J Biol VL - 78 IS - 2 N2 - Salacia crassifolia (Mart. Ex. Schult.) G. Don. is a bush which belongs to Celastraceae family and occurs specially in Brazilian Cerrado. Its leaves, stem, seeds and fruits are popularly used for several medicinal purposes, such as antitumoral, antirheumatic, anti-inflammatory and antimicrobial. In this study, the mutagenic and antimutagenic activities of S. crassifolia stem bark fractions (hexane, ethyl acetate and hydroalcoholic) were evaluated by the Ames mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. By the obtained results, all S. crassifolia fractions did not significantly increase the number of prototrophic revertants for histidine (His+) in both S. typhimurium strains tested (p > 0.05), suggesting absence of mutagenicity. Regarding antimutagenicity, the fractions ethyl acetate and hydroalcoholic significantly decreased the number of His+ revertants colonies induced by positive control for strain TA98 (p < 0.05), demonstrating protection against mutagenicity induced by 4-nitroquinolile1-oxide, whereas the hexane fraction did not show antimutagenic effect in this strain. In the TA100 strain, all fractions of S. crassifolia protected DNA against the harmful action of sodium azide, and the hexane fraction exhibited the greatest protection in this work. Thus, it's possible conclude that the fractions of S. crassifolia tested in this study could be used in chemoprevention. SN - 1678-4375 UR - https://www.unboundmedicine.com/medline/citation/28954013/Mutagenicity_and_antimutagenicity_of_Salacia_crassifolia__mart__Ex__Schult___G__Don__evaluated_by_Ames_test_ DB - PRIME DP - Unbound Medicine ER -