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Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) - An important underutilized plant.
Biochem Biophys Rep 2017; 12:79-90BB

Abstract

The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography- diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1- diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications.

Authors+Show Affiliations

Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415001, India.Department of Biotechnology, Shivaji University, Kolhapur 416004, India.Amity Institute of Biotechnology, Amity University, Mumbai 410206, India.Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415001, India. Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara 415001, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28955795

Citation

Ghadage, Dhanaji M., et al. "Extraction Efficiency, Phytochemical Profiles and Antioxidative Properties of Different Parts of Saptarangi (Salacia Chinensis L.) - an Important Underutilized Plant." Biochemistry and Biophysics Reports, vol. 12, 2017, pp. 79-90.
Ghadage DM, Kshirsagar PR, Pai SR, et al. Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) - An important underutilized plant. Biochem Biophys Rep. 2017;12:79-90.
Ghadage, D. M., Kshirsagar, P. R., Pai, S. R., & Chavan, J. J. (2017). Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) - An important underutilized plant. Biochemistry and Biophysics Reports, 12, pp. 79-90. doi:10.1016/j.bbrep.2017.08.012.
Ghadage DM, et al. Extraction Efficiency, Phytochemical Profiles and Antioxidative Properties of Different Parts of Saptarangi (Salacia Chinensis L.) - an Important Underutilized Plant. Biochem Biophys Rep. 2017;12:79-90. PubMed PMID: 28955795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.) - An important underutilized plant. AU - Ghadage,Dhanaji M, AU - Kshirsagar,Parthraj R, AU - Pai,Sandeep R, AU - Chavan,Jaykumar J, Y1 - 2017/08/24/ PY - 2016/11/19/received PY - 2017/06/19/revised PY - 2017/08/22/accepted PY - 2017/9/29/entrez PY - 2017/9/29/pubmed PY - 2017/9/29/medline KW - ABTS, 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) KW - AC, Antioxidant capacity KW - AEAC, ascorbic acid equivalent antioxidant capacity KW - Antioxidative capacity KW - CSE, continuous shaking extraction KW - DAD, diode array detector KW - DMPD, N, N-dimethyl-p-phenylenediamine KW - DPPH, 1, 1- diphenyl-1-picryl hydrazyl KW - DW, Dry weight KW - EAE, ellagic acid equivalent KW - Extraction optimization KW - FRAP, 2, 4, 6-tris (2-pyridyl)-s-triazine KW - Flavonoids KW - GAE, gallic acid equivalent KW - GC-MS KW - GC-MS, gas chromatography-mass spectroscopy KW - LOD, limit of detection KW - LOQ, limit of quantification KW - MAE, microwave assisted extraction KW - Minerals KW - NIST, national institute of standards KW - Phenolic acids KW - Phytoconstituents KW - QE, quercetin equivalent KW - RP-HPLC, reversed phase-high performance liquid chromatography KW - RP-HPLC-DAD KW - SBAE, steam bath assisted extraction KW - Salacia chinensis KW - TAE, tannic acid equivalent KW - TEAC, trolox equivalent antioxidant capacity KW - TFC, total flavonoid content KW - TIC, total ion chromatogram KW - TPC, total phenolic content KW - UE, ultrasonic extraction SP - 79 EP - 90 JF - Biochemistry and biophysics reports JO - Biochem Biophys Rep VL - 12 N2 - The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography- diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1- diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications. SN - 2405-5808 UR - https://www.unboundmedicine.com/medline/citation/28955795/Extraction_efficiency_phytochemical_profiles_and_antioxidative_properties_of_different_parts_of_Saptarangi__Salacia_chinensis_L_____An_important_underutilized_plant_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2405-5808(16)30302-8 DB - PRIME DP - Unbound Medicine ER -