Tags

Type your tag names separated by a space and hit enter

Application of gas chromatography-tandem mass spectrometry for the determination of amphetamine-type stimulants in blood and urine.
J Pharm Biomed Anal. 2018 Jan 30; 148:58-64.JP

Abstract

Amphetamine, methamphetamine, phentermine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxy-N-ethylamphetamine (MDEA) are the most popular amphetamine-type stimulants. The use of these substances is a serious societal problem worldwide. In this study, a method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) with simple and rapid liquid-liquid extraction (LLE) and derivatization was developed and validated for the simultaneous determination of the six aforementioned amphetamine derivatives in blood and urine. The detection of all compounds was based on multiple reaction monitoring (MRM) transitions. The most important advantage of the method is the minimal sample volume (as low as 200μL) required for the extraction procedure. The validation parameters, i.e., the recovery (90.5-104%), inter-day accuracy (94.2-109.1%) and precision (0.5-5.8%), showed the repeatability and sensitivity of the method for both matrices and indicated that the proposed procedure fulfils internationally established acceptance criteria for bioanalytical methods The procedure was successfully applied to the analysis of real blood and urine samples examined in 22 forensic toxicological cases. To the best of our knowledge, this is the first work presenting the use of GC-MS/MS for the determination of amphetamine-type stimulants in blood and urine. In view of the low limits of detection (0.09-0.81ng/mL), limits of quantification (0.26-2.4ng/mL), and high selectivity, the procedure can be applied for drug monitoring in both fatal and non-fatal intoxication cases in routine toxicology analysis.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza Str., Gdańsk 80-233, Poland. Electronic address: womat90@gmail.com.Department of Forensic Medicine, Faculty of Medicine, Medical University of Gdańsk, 3A Marii Skłodowskiej-Curie Str., Gdańsk 80-210, Poland.Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza Str., Gdańsk 80-233, Poland.Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza Str., Gdańsk 80-233, Poland.Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza Str., Gdańsk 80-233, Poland.Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 Narutowicza Str., Gdańsk 80-233, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28957720

Citation

Woźniak, Mateusz Kacper, et al. "Application of Gas Chromatography-tandem Mass Spectrometry for the Determination of Amphetamine-type Stimulants in Blood and Urine." Journal of Pharmaceutical and Biomedical Analysis, vol. 148, 2018, pp. 58-64.
Woźniak MK, Wiergowski M, Aszyk J, et al. Application of gas chromatography-tandem mass spectrometry for the determination of amphetamine-type stimulants in blood and urine. J Pharm Biomed Anal. 2018;148:58-64.
Woźniak, M. K., Wiergowski, M., Aszyk, J., Kubica, P., Namieśnik, J., & Biziuk, M. (2018). Application of gas chromatography-tandem mass spectrometry for the determination of amphetamine-type stimulants in blood and urine. Journal of Pharmaceutical and Biomedical Analysis, 148, 58-64. https://doi.org/10.1016/j.jpba.2017.09.020
Woźniak MK, et al. Application of Gas Chromatography-tandem Mass Spectrometry for the Determination of Amphetamine-type Stimulants in Blood and Urine. J Pharm Biomed Anal. 2018 Jan 30;148:58-64. PubMed PMID: 28957720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of gas chromatography-tandem mass spectrometry for the determination of amphetamine-type stimulants in blood and urine. AU - Woźniak,Mateusz Kacper, AU - Wiergowski,Marek, AU - Aszyk,Justyna, AU - Kubica,Paweł, AU - Namieśnik,Jacek, AU - Biziuk,Marek, Y1 - 2017/09/18/ PY - 2017/07/14/received PY - 2017/09/13/revised PY - 2017/09/15/accepted PY - 2017/9/29/pubmed PY - 2018/7/4/medline PY - 2017/9/29/entrez KW - Amphetamine-type stimulants (ATS) KW - Biological specimens KW - Drug analysis KW - GC–MS/MS KW - Toxicological screening SP - 58 EP - 64 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 148 N2 - Amphetamine, methamphetamine, phentermine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxy-N-ethylamphetamine (MDEA) are the most popular amphetamine-type stimulants. The use of these substances is a serious societal problem worldwide. In this study, a method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) with simple and rapid liquid-liquid extraction (LLE) and derivatization was developed and validated for the simultaneous determination of the six aforementioned amphetamine derivatives in blood and urine. The detection of all compounds was based on multiple reaction monitoring (MRM) transitions. The most important advantage of the method is the minimal sample volume (as low as 200μL) required for the extraction procedure. The validation parameters, i.e., the recovery (90.5-104%), inter-day accuracy (94.2-109.1%) and precision (0.5-5.8%), showed the repeatability and sensitivity of the method for both matrices and indicated that the proposed procedure fulfils internationally established acceptance criteria for bioanalytical methods The procedure was successfully applied to the analysis of real blood and urine samples examined in 22 forensic toxicological cases. To the best of our knowledge, this is the first work presenting the use of GC-MS/MS for the determination of amphetamine-type stimulants in blood and urine. In view of the low limits of detection (0.09-0.81ng/mL), limits of quantification (0.26-2.4ng/mL), and high selectivity, the procedure can be applied for drug monitoring in both fatal and non-fatal intoxication cases in routine toxicology analysis. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/28957720/Application_of_gas_chromatography_tandem_mass_spectrometry_for_the_determination_of_amphetamine_type_stimulants_in_blood_and_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)31816-2 DB - PRIME DP - Unbound Medicine ER -