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Structural characterization of a new water-soluble polysaccharide isolated from Acanthophyllum acerosum roots and its antioxidant activity.
Int J Biol Macromol. 2018 Feb; 107(Pt A):1227-1234.IJ

Abstract

A new water-soluble polysaccharide named as AAPS-1, was extracted with warm water from the roots of Acanthophyllum acerosum and further purified by DEAE-cellulose A52 anion-exchange and Sephadex G-50 gel filtration chromatography. AAPS-1 was a neutral polysaccharide, with a molecular weight of 23.2kDa and a specific optical rotation of +181.3° (c 1.0, H2O), and comprised of Glucose, Galactose and Arabinose with a relative molar ratio of 1.6:5.1:1.0. Structure features of AAPS-1 were investigated by a combination of chemical and instrumental analysis, such as partial acid hydrolysis, methylation, periodate oxidation and Smith degradation, GC-MS, FTIR and NMR (13C and 1H). The data obtained indicate that AAPS-1 possessed a backbone of →6)-α-d-Galp-(1→ residues, with branches attached to O-2 (∼35%) by α-d-Glcp-(1→ and at O-3 (∼16%) by α-d-Galp-(1→ and by β-l-Arap-(1→3)-β-l-Arap-(1→3)-β-l-Arap-(1→. The scavenging activity of AAPS-1 against DPPH radical was less than that of ascorbic acid at the same concentrations and the EC50 value of AAPS-1 was 1.4mg/ml.

Authors+Show Affiliations

Shahrood University of Technology, School of Agricultural Engineering, Department of Food Science and Technology, P.O. Box 361999-5161, Shahrood, Iran. Electronic address: Jahanbin@shahroodut.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28958818

Citation

Jahanbin, Kambiz. "Structural Characterization of a New Water-soluble Polysaccharide Isolated From Acanthophyllum Acerosum Roots and Its Antioxidant Activity." International Journal of Biological Macromolecules, vol. 107, no. Pt A, 2018, pp. 1227-1234.
Jahanbin K. Structural characterization of a new water-soluble polysaccharide isolated from Acanthophyllum acerosum roots and its antioxidant activity. Int J Biol Macromol. 2018;107(Pt A):1227-1234.
Jahanbin, K. (2018). Structural characterization of a new water-soluble polysaccharide isolated from Acanthophyllum acerosum roots and its antioxidant activity. International Journal of Biological Macromolecules, 107(Pt A), 1227-1234. https://doi.org/10.1016/j.ijbiomac.2017.09.100
Jahanbin K. Structural Characterization of a New Water-soluble Polysaccharide Isolated From Acanthophyllum Acerosum Roots and Its Antioxidant Activity. Int J Biol Macromol. 2018;107(Pt A):1227-1234. PubMed PMID: 28958818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterization of a new water-soluble polysaccharide isolated from Acanthophyllum acerosum roots and its antioxidant activity. A1 - Jahanbin,Kambiz, Y1 - 2017/09/25/ PY - 2017/06/09/received PY - 2017/09/03/revised PY - 2017/09/25/accepted PY - 2017/9/30/pubmed PY - 2018/7/24/medline PY - 2017/9/30/entrez KW - Acanthophyllum acerosum KW - Isolation KW - Polysaccharide KW - Radical-scavenging activity KW - Structural study SP - 1227 EP - 1234 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 107 IS - Pt A N2 - A new water-soluble polysaccharide named as AAPS-1, was extracted with warm water from the roots of Acanthophyllum acerosum and further purified by DEAE-cellulose A52 anion-exchange and Sephadex G-50 gel filtration chromatography. AAPS-1 was a neutral polysaccharide, with a molecular weight of 23.2kDa and a specific optical rotation of +181.3° (c 1.0, H2O), and comprised of Glucose, Galactose and Arabinose with a relative molar ratio of 1.6:5.1:1.0. Structure features of AAPS-1 were investigated by a combination of chemical and instrumental analysis, such as partial acid hydrolysis, methylation, periodate oxidation and Smith degradation, GC-MS, FTIR and NMR (13C and 1H). The data obtained indicate that AAPS-1 possessed a backbone of →6)-α-d-Galp-(1→ residues, with branches attached to O-2 (∼35%) by α-d-Glcp-(1→ and at O-3 (∼16%) by α-d-Galp-(1→ and by β-l-Arap-(1→3)-β-l-Arap-(1→3)-β-l-Arap-(1→. The scavenging activity of AAPS-1 against DPPH radical was less than that of ascorbic acid at the same concentrations and the EC50 value of AAPS-1 was 1.4mg/ml. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28958818/Structural_characterization_of_a_new_water_soluble_polysaccharide_isolated_from_Acanthophyllum_acerosum_roots_and_its_antioxidant_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32077-9 DB - PRIME DP - Unbound Medicine ER -