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Flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen doped carbon nanotube arrays: In situ electrochemical detection in live cancer cells.
Biosens Bioelectron. 2018 Feb 15; 100:453-461.BB

Abstract

The rapidly growing demand for in situ real-time monitoring of chemical information in vitro and in vivo has attracted tremendous research efforts into the design and construction of high-performance biosensor devices. Herein, we develop a new type of flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen-doped carbon nanotube arrays, and explore its practical application in in situ electrochemical detection of cancer biomarker H2O2 secreted from live cancer cells. Our results demonstrate that carbon fiber material with microscale size and fascinating mechanical properties can be used as a robust and flexible microelectrode substrate in the electrochemical biosensor system. And the highly ordered nitrogen-doped carbon nanotube arrays that grown on carbon fiber possess high surface area-to-volume ratio and abundant active sites, which facilitate the loading of high-density and uniformly dispersed gold nanoparticles on it. Benefited from the unique microstructure and excellent electrocatalytic properties of different components in the nanohybrid fiber microelectrode, an effective electrochemical sensing platform based on it has been built up for the sensitive and selective detection of H2O2, the detection limit is calculated to be 50nM when the signal-to-noise ratio is 3:1, and the linear dynamic range is up to 4.3mM, with a high sensitivity of 142µAcm-2mM-1. These good sensing performances, coupled with its intrinsic mechanical flexibility and biocompatibility, allow for its use in in situ real-time tracking H2O2 secreted from breast cancer cell lines MCF-7 and MBA-MD-231, and evaluating the sensitivity of different cancer cells to chemotherapy or radiotherapy treatments, which hold great promise for clinic application in cancer diagnose and management.

Authors+Show Affiliations

Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430073, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China. Electronic address: xiaofei@hust.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28963962

Citation

Zhang, Yan, et al. "Flexible Nanohybrid Microelectrode Based On Carbon Fiber Wrapped By Gold Nanoparticles Decorated Nitrogen Doped Carbon Nanotube Arrays: in Situ Electrochemical Detection in Live Cancer Cells." Biosensors & Bioelectronics, vol. 100, 2018, pp. 453-461.
Zhang Y, Xiao J, Sun Y, et al. Flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen doped carbon nanotube arrays: In situ electrochemical detection in live cancer cells. Biosens Bioelectron. 2018;100:453-461.
Zhang, Y., Xiao, J., Sun, Y., Wang, L., Dong, X., Ren, J., He, W., & Xiao, F. (2018). Flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen doped carbon nanotube arrays: In situ electrochemical detection in live cancer cells. Biosensors & Bioelectronics, 100, 453-461. https://doi.org/10.1016/j.bios.2017.09.038
Zhang Y, et al. Flexible Nanohybrid Microelectrode Based On Carbon Fiber Wrapped By Gold Nanoparticles Decorated Nitrogen Doped Carbon Nanotube Arrays: in Situ Electrochemical Detection in Live Cancer Cells. Biosens Bioelectron. 2018 Feb 15;100:453-461. PubMed PMID: 28963962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen doped carbon nanotube arrays: In situ electrochemical detection in live cancer cells. AU - Zhang,Yan, AU - Xiao,Jian, AU - Sun,Yimin, AU - Wang,Lu, AU - Dong,Xulin, AU - Ren,Jinghua, AU - He,Wenshan, AU - Xiao,Fei, Y1 - 2017/09/22/ PY - 2017/09/07/received PY - 2017/09/20/accepted PY - 2017/10/1/pubmed PY - 2018/6/26/medline PY - 2017/10/1/entrez KW - Cancer cells KW - Electrochemical biosensors KW - Flexible fiber microelectrode KW - Gold nanoparticles KW - Nitrogen doped carbon nanotube arrays KW - in situ detection SP - 453 EP - 461 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 100 N2 - The rapidly growing demand for in situ real-time monitoring of chemical information in vitro and in vivo has attracted tremendous research efforts into the design and construction of high-performance biosensor devices. Herein, we develop a new type of flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen-doped carbon nanotube arrays, and explore its practical application in in situ electrochemical detection of cancer biomarker H2O2 secreted from live cancer cells. Our results demonstrate that carbon fiber material with microscale size and fascinating mechanical properties can be used as a robust and flexible microelectrode substrate in the electrochemical biosensor system. And the highly ordered nitrogen-doped carbon nanotube arrays that grown on carbon fiber possess high surface area-to-volume ratio and abundant active sites, which facilitate the loading of high-density and uniformly dispersed gold nanoparticles on it. Benefited from the unique microstructure and excellent electrocatalytic properties of different components in the nanohybrid fiber microelectrode, an effective electrochemical sensing platform based on it has been built up for the sensitive and selective detection of H2O2, the detection limit is calculated to be 50nM when the signal-to-noise ratio is 3:1, and the linear dynamic range is up to 4.3mM, with a high sensitivity of 142µAcm-2mM-1. These good sensing performances, coupled with its intrinsic mechanical flexibility and biocompatibility, allow for its use in in situ real-time tracking H2O2 secreted from breast cancer cell lines MCF-7 and MBA-MD-231, and evaluating the sensitivity of different cancer cells to chemotherapy or radiotherapy treatments, which hold great promise for clinic application in cancer diagnose and management. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28963962/Flexible_nanohybrid_microelectrode_based_on_carbon_fiber_wrapped_by_gold_nanoparticles_decorated_nitrogen_doped_carbon_nanotube_arrays:_In_situ_electrochemical_detection_in_live_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30647-4 DB - PRIME DP - Unbound Medicine ER -