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Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent.
J Ethnopharmacol. 2018 Jan 30; 211:197-206.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Physalis alkekengi L. var. franchetii (Solanaceae) has been widely used in Chinese folk medicine due to its wide distribution throughout the country, for the treatment of a wide range of diseases including heat and cold, sore throat, fever, fungal infection, inflammation, toothache, rheumatism, burn, analgesic, ulcer and urinary diseases. However, the effect of P. alkekengi var. franchetii on ulcer and Helicobacter pylori infection has not been reported to date.

AIM OF THE STUDY

This study was designed to investigate the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of ethyl acetate fraction of the crude aqueous methanolic extract from the aerial parts of the plant P. alkekengi L. var. franchetii in rodents.

MATERIALS AND METHODS

Acute toxicity of the crude extract of P. alkekengi L. var. franchetii (PAF) was evaluated in rats. The petroleum ether fraction (PEF), butanol fraction (BF), ethyl acetate fraction (EAF) and aqueous fraction (AF) of crude aqueous methanolic extract from PAF were screened for anti-inflammatory and anti-ulcer potential at doses of 100, 250 and 500mg/kg (p.o.), using carrageenin-induced hind paw edema and ethanol-induced gastric lesions test in rats. In vitro anti-Helicobacter pylori activity of EAF was assayed subsequently. In addition, three doses of EAF were evaluated for analgesic activity using hot plate and writhing tests, respectively. Finally, we performed a phytochemical analysis of EAF.

RESULTS

Four fractions of crude extract from PAF significantly reduced the paw volume in carrageenin-induced hind paw edema model at different doses (100, 250 and 500mg/kg, p.o.). The fraction EAF at a dose of 500mg/kg exhibited the highest (75.92%) (0.150 ± 0.045***, ***p < 0.001) anti-inflammatory potential, which is similar to indomethacin (***P < 0.001)(0.120 ± 0.014***, 80.74% inhibition of inflammation) at 5mg/kg. Pretreatment with EAF (500mg/kg, p.o.) significantly reduced the intensity of gastric mucosal damage and showed higher gastroprotective activity (90.6%) when compared to the standard drug famotidine (84.6%). In addition, EAF fraction also showed a moderate (P < 0.05) anti-Helicobacter pylori activity with a minimal inhibition concentration (MIC) of 500μg/ml. Furthermore, pain sensation was effectively inhibited at 500mg/kg, p.o. of EAF as manifested by an increase (p < 0.001) of latency time in hot plate from 30 to 90min and a decrease (p < 0.001) in count of writhing induced by acetic acid. By HPLC, we determined some steroid, terpenoid and flavonoids (four compounds): kaempferol, quercetin, Blumenol A and physalindicanols A, which were isolated from the ethyl acetate fraction and identified using 1H NMR and 13C NMR spectra analysis.

CONCLUSION

This study demonstrated the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of EAF of the crude extract from PAF thus justifying its traditional usage.

Authors+Show Affiliations

Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 TongJiaXiang, Nanjing 210009, Jiangsu Province, PR China; Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China. Electronic address: yaoxue604@163.com.Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 TongJiaXiang, Nanjing 210009, Jiangsu Province, PR China. Electronic address: cpuwsl@126.com.Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.Department of Scientific Research, Guizhou Provincial Institute for Food and Drug Control, 142 Shibei Road, Guiyang 550004, Guizhou Province, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28964871

Citation

Wang, Yong, et al. "Anti-ulcer and anti-Helicobacter Pylori Potentials of the Ethyl Acetate Fraction of Physalis Alkekengi L. Var. Franchetii (Solanaceae) in Rodent." Journal of Ethnopharmacology, vol. 211, 2018, pp. 197-206.
Wang Y, Wang SL, Zhang JY, et al. Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent. J Ethnopharmacol. 2018;211:197-206.
Wang, Y., Wang, S. L., Zhang, J. Y., Song, X. N., Zhang, Z. Y., Li, J. F., & Li, S. (2018). Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent. Journal of Ethnopharmacology, 211, 197-206. https://doi.org/10.1016/j.jep.2017.09.004
Wang Y, et al. Anti-ulcer and anti-Helicobacter Pylori Potentials of the Ethyl Acetate Fraction of Physalis Alkekengi L. Var. Franchetii (Solanaceae) in Rodent. J Ethnopharmacol. 2018 Jan 30;211:197-206. PubMed PMID: 28964871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-ulcer and anti-Helicobacter pylori potentials of the ethyl acetate fraction of Physalis alkekengi L. var. franchetii (Solanaceae) in rodent. AU - Wang,Yong, AU - Wang,Sui Lou, AU - Zhang,Jiong Yi, AU - Song,Xiao Ning, AU - Zhang,Zhi Yong, AU - Li,Jing Feng, AU - Li,Song, Y1 - 2017/09/28/ PY - 2016/08/31/received PY - 2017/07/04/revised PY - 2017/09/07/accepted PY - 2017/10/2/pubmed PY - 2018/7/22/medline PY - 2017/10/2/entrez KW - Analgesic KW - Anti-Helicobacter pylori KW - Anti-inflammatory KW - Anti-ulcer KW - Physalis alkekengi L. var. franchetii SP - 197 EP - 206 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 211 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Physalis alkekengi L. var. franchetii (Solanaceae) has been widely used in Chinese folk medicine due to its wide distribution throughout the country, for the treatment of a wide range of diseases including heat and cold, sore throat, fever, fungal infection, inflammation, toothache, rheumatism, burn, analgesic, ulcer and urinary diseases. However, the effect of P. alkekengi var. franchetii on ulcer and Helicobacter pylori infection has not been reported to date. AIM OF THE STUDY: This study was designed to investigate the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of ethyl acetate fraction of the crude aqueous methanolic extract from the aerial parts of the plant P. alkekengi L. var. franchetii in rodents. MATERIALS AND METHODS: Acute toxicity of the crude extract of P. alkekengi L. var. franchetii (PAF) was evaluated in rats. The petroleum ether fraction (PEF), butanol fraction (BF), ethyl acetate fraction (EAF) and aqueous fraction (AF) of crude aqueous methanolic extract from PAF were screened for anti-inflammatory and anti-ulcer potential at doses of 100, 250 and 500mg/kg (p.o.), using carrageenin-induced hind paw edema and ethanol-induced gastric lesions test in rats. In vitro anti-Helicobacter pylori activity of EAF was assayed subsequently. In addition, three doses of EAF were evaluated for analgesic activity using hot plate and writhing tests, respectively. Finally, we performed a phytochemical analysis of EAF. RESULTS: Four fractions of crude extract from PAF significantly reduced the paw volume in carrageenin-induced hind paw edema model at different doses (100, 250 and 500mg/kg, p.o.). The fraction EAF at a dose of 500mg/kg exhibited the highest (75.92%) (0.150 ± 0.045***, ***p < 0.001) anti-inflammatory potential, which is similar to indomethacin (***P < 0.001)(0.120 ± 0.014***, 80.74% inhibition of inflammation) at 5mg/kg. Pretreatment with EAF (500mg/kg, p.o.) significantly reduced the intensity of gastric mucosal damage and showed higher gastroprotective activity (90.6%) when compared to the standard drug famotidine (84.6%). In addition, EAF fraction also showed a moderate (P < 0.05) anti-Helicobacter pylori activity with a minimal inhibition concentration (MIC) of 500μg/ml. Furthermore, pain sensation was effectively inhibited at 500mg/kg, p.o. of EAF as manifested by an increase (p < 0.001) of latency time in hot plate from 30 to 90min and a decrease (p < 0.001) in count of writhing induced by acetic acid. By HPLC, we determined some steroid, terpenoid and flavonoids (four compounds): kaempferol, quercetin, Blumenol A and physalindicanols A, which were isolated from the ethyl acetate fraction and identified using 1H NMR and 13C NMR spectra analysis. CONCLUSION: This study demonstrated the anti-inflammatory, anti-ulcer, anti-Helicobacter pylori and analgesic properties of EAF of the crude extract from PAF thus justifying its traditional usage. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/28964871/Anti_ulcer_and_anti_Helicobacter_pylori_potentials_of_the_ethyl_acetate_fraction_of_Physalis_alkekengi_L__var__franchetii__Solanaceae__in_rodent_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30613-4 DB - PRIME DP - Unbound Medicine ER -