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Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice.
J Nutr Biochem. 2017 12; 50:16-25.JN

Abstract

Obesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance.

Authors+Show Affiliations

Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Physical Education, São Paulo State University (UNESP), Bioscience Institute, Rio Claro, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Biology Science, Federal University of Pernambuco, Recife, PE, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil.Department of Internal Medicine, State University of Campinas, 13081-970, Campinas, SP, Brazil. Electronic address: msaad@fcm.unicamp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28968517

Citation

Bagarolli, Renata A., et al. "Probiotics Modulate Gut Microbiota and Improve Insulin Sensitivity in DIO Mice." The Journal of Nutritional Biochemistry, vol. 50, 2017, pp. 16-25.
Bagarolli RA, Tobar N, Oliveira AG, et al. Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice. J Nutr Biochem. 2017;50:16-25.
Bagarolli, R. A., Tobar, N., Oliveira, A. G., Araújo, T. G., Carvalho, B. M., Rocha, G. Z., Vecina, J. F., Calisto, K., Guadagnini, D., Prada, P. O., Santos, A., Saad, S. T. O., & Saad, M. J. A. (2017). Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice. The Journal of Nutritional Biochemistry, 50, 16-25. https://doi.org/10.1016/j.jnutbio.2017.08.006
Bagarolli RA, et al. Probiotics Modulate Gut Microbiota and Improve Insulin Sensitivity in DIO Mice. J Nutr Biochem. 2017;50:16-25. PubMed PMID: 28968517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice. AU - Bagarolli,Renata A, AU - Tobar,Natália, AU - Oliveira,Alexandre G, AU - Araújo,Tiago G, AU - Carvalho,Bruno M, AU - Rocha,Guilherme Z, AU - Vecina,Juliana F, AU - Calisto,Kelly, AU - Guadagnini,Dioze, AU - Prada,Patrícia O, AU - Santos,Andrey, AU - Saad,Sara T O, AU - Saad,Mario J A, Y1 - 2017/08/26/ PY - 2017/03/09/received PY - 2017/07/05/revised PY - 2017/08/17/accepted PY - 2017/10/3/pubmed PY - 2018/5/1/medline PY - 2017/10/3/entrez KW - Gut microbiota KW - Insulin sensitivity KW - Probiotics SP - 16 EP - 25 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 50 N2 - Obesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/28968517/Probiotics_modulate_gut_microbiota_and_improve_insulin_sensitivity_in_DIO_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(17)30187-0 DB - PRIME DP - Unbound Medicine ER -