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Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H- Isolates from Czech Patients with Novel Plasmid Composition Not Previously Seen in German Isolates.
Appl Environ Microbiol. 2017 Dec 01; 83(23)AE

Abstract

Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H- strains, first identified in Germany, have emerged as important pathogens throughout Europe. Besides chromosomally encoded Shiga toxin 2a (the major virulence factor), several putative virulence loci, including the hly, etp, and sfp operons, encoding EHEC hemolysin, type II secretion system proteins, and Sfp fimbriae, respectively, are located on the 121-kb plasmid pSFO157 in German strains. Here we report novel SF EHEC O157:H- strains isolated from patients in the Czech Republic. These strains share the core genomes and chromosomal virulence loci encoding toxins (stx2a and the cdtV-ABC operon) and adhesins (eae-γ, efa1, lpfAO157OI-141, and lpfAO157OI-154) with German strains but differ essentially in their plasmids. In contrast to all previously detected SF EHEC O157:H- strains, the Czech strains carry two plasmids, of 79 kb and 86 kb. The 79-kb plasmid harbors the sfp operon, but neither of the plasmids contains the hly and etp operons. Sequence analyses demonstrated that the 79-kb plasmid (pSFO157 258/98-1) evolved from pSFO157 of German strains by deletion of a 41,534-bp region via homologous recombination, resulting in loss of the hly and etp operons. The 86-kb plasmid (pSFO157 258/98-2) displays 98% sequence similarity to a 92.7-kb plasmid of an extraintestinal pathogenic E. coli bloodstream isolate. Our finding of this novel plasmid composition in SF EHEC O157:H- strains extends the evolutionary history of EHEC O157 plasmids. Moreover, the unique molecular plasmid characteristics permit the identification of such strains, thereby facilitating further investigations of their geographic distribution, clinical significance, and epidemiology.IMPORTANCE Since their first identification in Germany in 1989, sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- (nonmotile) strains have emerged as important causes of the life-threatening disease hemolytic-uremic syndrome in Europe. They account for 10 to 20% of sporadic cases of this disease and have caused several large outbreaks. The strains isolated throughout Europe share conserved chromosomal and plasmid characteristics. Here we identified novel sorbitol-fermenting enterohemorrhagic E. coli O157:H- patient isolates in the Czech Republic which differ from all such strains reported previously by their unique plasmid characteristics, including plasmid number, composition of plasmid-carried virulence genes, and plasmid origins. Our findings contribute substantially to understanding the evolution of E. coli O157 strains and their plasmids. In practical terms, they enable the identification of strains with these novel plasmid characteristics in patient stool samples and thus the investigation of their roles as human pathogens in other geographic areas.

Authors+Show Affiliations

Institute for Hygiene, University of Münster, Münster, Germany.National Reference Laboratory for E. coli and Shigella, National Institute of Public Health, Prague, Czech Republic.Institute for Hygiene, University of Münster, Münster, Germany.National Reference Center for Salmonella and Other Enteric Pathogens, Robert Koch Institut, Wernigerode, Germany.Institute for Hygiene, University of Münster, Münster, Germany.Institute for Hygiene, University of Münster, Münster, Germany.Institute for Hygiene, University of Münster, Münster, Germany.Institute for Hygiene, University of Münster, Münster, Germany.Institute for Hygiene, University of Münster, Münster, Germany mbiela@uni-muenster.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28970221

Citation

Bauwens, Andreas, et al. "Sorbitol-Fermenting Enterohemorrhagic Escherichia Coli O157:H- Isolates From Czech Patients With Novel Plasmid Composition Not Previously Seen in German Isolates." Applied and Environmental Microbiology, vol. 83, no. 23, 2017.
Bauwens A, Marejková M, Middendorf-Bauchart B, et al. Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H- Isolates from Czech Patients with Novel Plasmid Composition Not Previously Seen in German Isolates. Appl Environ Microbiol. 2017;83(23).
Bauwens, A., Marejková, M., Middendorf-Bauchart, B., Prager, R., Kossow, A., Zhang, W., Karch, H., Mellmann, A., & Bielaszewska, M. (2017). Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H- Isolates from Czech Patients with Novel Plasmid Composition Not Previously Seen in German Isolates. Applied and Environmental Microbiology, 83(23). https://doi.org/10.1128/AEM.01454-17
Bauwens A, et al. Sorbitol-Fermenting Enterohemorrhagic Escherichia Coli O157:H- Isolates From Czech Patients With Novel Plasmid Composition Not Previously Seen in German Isolates. Appl Environ Microbiol. 2017 Dec 1;83(23) PubMed PMID: 28970221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H- Isolates from Czech Patients with Novel Plasmid Composition Not Previously Seen in German Isolates. AU - Bauwens,Andreas, AU - Marejková,Monika, AU - Middendorf-Bauchart,Barbara, AU - Prager,Rita, AU - Kossow,Annelene, AU - Zhang,Wenlan, AU - Karch,Helge, AU - Mellmann,Alexander, AU - Bielaszewska,Martina, Y1 - 2017/11/16/ PY - 2017/07/04/received PY - 2017/09/21/accepted PY - 2017/10/4/pubmed PY - 2017/12/15/medline PY - 2017/10/4/entrez KW - EHEC O157 KW - Sfp fimbriae KW - enterohemorrhagic E. coli KW - hemolytic-uremic syndrome KW - outbreaks KW - plasmid analysis JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 83 IS - 23 N2 - Sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H- strains, first identified in Germany, have emerged as important pathogens throughout Europe. Besides chromosomally encoded Shiga toxin 2a (the major virulence factor), several putative virulence loci, including the hly, etp, and sfp operons, encoding EHEC hemolysin, type II secretion system proteins, and Sfp fimbriae, respectively, are located on the 121-kb plasmid pSFO157 in German strains. Here we report novel SF EHEC O157:H- strains isolated from patients in the Czech Republic. These strains share the core genomes and chromosomal virulence loci encoding toxins (stx2a and the cdtV-ABC operon) and adhesins (eae-γ, efa1, lpfAO157OI-141, and lpfAO157OI-154) with German strains but differ essentially in their plasmids. In contrast to all previously detected SF EHEC O157:H- strains, the Czech strains carry two plasmids, of 79 kb and 86 kb. The 79-kb plasmid harbors the sfp operon, but neither of the plasmids contains the hly and etp operons. Sequence analyses demonstrated that the 79-kb plasmid (pSFO157 258/98-1) evolved from pSFO157 of German strains by deletion of a 41,534-bp region via homologous recombination, resulting in loss of the hly and etp operons. The 86-kb plasmid (pSFO157 258/98-2) displays 98% sequence similarity to a 92.7-kb plasmid of an extraintestinal pathogenic E. coli bloodstream isolate. Our finding of this novel plasmid composition in SF EHEC O157:H- strains extends the evolutionary history of EHEC O157 plasmids. Moreover, the unique molecular plasmid characteristics permit the identification of such strains, thereby facilitating further investigations of their geographic distribution, clinical significance, and epidemiology.IMPORTANCE Since their first identification in Germany in 1989, sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- (nonmotile) strains have emerged as important causes of the life-threatening disease hemolytic-uremic syndrome in Europe. They account for 10 to 20% of sporadic cases of this disease and have caused several large outbreaks. The strains isolated throughout Europe share conserved chromosomal and plasmid characteristics. Here we identified novel sorbitol-fermenting enterohemorrhagic E. coli O157:H- patient isolates in the Czech Republic which differ from all such strains reported previously by their unique plasmid characteristics, including plasmid number, composition of plasmid-carried virulence genes, and plasmid origins. Our findings contribute substantially to understanding the evolution of E. coli O157 strains and their plasmids. In practical terms, they enable the identification of strains with these novel plasmid characteristics in patient stool samples and thus the investigation of their roles as human pathogens in other geographic areas. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/28970221/Sorbitol_Fermenting_Enterohemorrhagic_Escherichia_coli_O157:H__Isolates_from_Czech_Patients_with_Novel_Plasmid_Composition_Not_Previously_Seen_in_German_Isolates_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=28970221 DB - PRIME DP - Unbound Medicine ER -