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Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference.
Sci Rep. 2017 10 03; 7(1):12591.SR

Abstract

RNA interference (RNAi) in transgenic maize has recently emerged as an alternative mode of action for western corn rootworm (Diabrotica virgifera virgifera) control which can be combined with protein-based rootworm control options for improved root protection and resistance management. Currently, transgenic RNAi-based control has focused on suppression of genes that when silenced lead to larval mortality. We investigated control of western corn rootworm reproduction through RNAi by targeting two reproductive genes, dvvgr and dvbol, with the goal of reducing insect fecundity as a new tool for pest management. The results demonstrated that exposure of adult beetles, as well as larvae to dvvgr or dvbol dsRNA in artificial diet, caused reduction of fecundity. Furthermore, western corn rootworm beetles that emerged from larval feeding on transgenic maize roots expressing dvbol dsRNA also showed significant fecundity reduction. This is the first report of reduction of insect reproductive fitness through plant-mediated RNAi, demonstrating the feasibility of reproductive RNAi as a management tool for western corn rootworm.

Authors+Show Affiliations

DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA. xu.hu@pioneer.com.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA. Evogene Ltd, Saint Louis, MO, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.DuPont Pioneer, 7300 NW 62nd Ave., Johnston, IA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28974735

Citation

Niu, Xiping, et al. "Control of Western Corn Rootworm (Diabrotica Virgifera Virgifera) Reproduction Through Plant-Mediated RNA Interference." Scientific Reports, vol. 7, no. 1, 2017, p. 12591.
Niu X, Kassa A, Hu X, et al. Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference. Sci Rep. 2017;7(1):12591.
Niu, X., Kassa, A., Hu, X., Robeson, J., McMahon, M., Richtman, N. M., Steimel, J. P., Kernodle, B. M., Crane, V. C., Sandahl, G., Ritland, J. L., Presnail, J. K., Lu, A. L., & Wu, G. (2017). Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference. Scientific Reports, 7(1), 12591. https://doi.org/10.1038/s41598-017-12638-3
Niu X, et al. Control of Western Corn Rootworm (Diabrotica Virgifera Virgifera) Reproduction Through Plant-Mediated RNA Interference. Sci Rep. 2017 10 3;7(1):12591. PubMed PMID: 28974735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference. AU - Niu,Xiping, AU - Kassa,Adane, AU - Hu,Xu, AU - Robeson,Jonathan, AU - McMahon,Mollie, AU - Richtman,Nina M, AU - Steimel,Joseph P, AU - Kernodle,Bliss M, AU - Crane,Virginia C, AU - Sandahl,Gary, AU - Ritland,Julie L, AU - Presnail,James K, AU - Lu,Albert L, AU - Wu,Gusui, Y1 - 2017/10/03/ PY - 2017/05/30/received PY - 2017/09/13/accepted PY - 2017/10/5/entrez PY - 2017/10/5/pubmed PY - 2019/6/27/medline SP - 12591 EP - 12591 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - RNA interference (RNAi) in transgenic maize has recently emerged as an alternative mode of action for western corn rootworm (Diabrotica virgifera virgifera) control which can be combined with protein-based rootworm control options for improved root protection and resistance management. Currently, transgenic RNAi-based control has focused on suppression of genes that when silenced lead to larval mortality. We investigated control of western corn rootworm reproduction through RNAi by targeting two reproductive genes, dvvgr and dvbol, with the goal of reducing insect fecundity as a new tool for pest management. The results demonstrated that exposure of adult beetles, as well as larvae to dvvgr or dvbol dsRNA in artificial diet, caused reduction of fecundity. Furthermore, western corn rootworm beetles that emerged from larval feeding on transgenic maize roots expressing dvbol dsRNA also showed significant fecundity reduction. This is the first report of reduction of insect reproductive fitness through plant-mediated RNAi, demonstrating the feasibility of reproductive RNAi as a management tool for western corn rootworm. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28974735/Control_of_Western_Corn_Rootworm__Diabrotica_virgifera_virgifera__Reproduction_through_Plant_Mediated_RNA_Interference_ L2 - https://doi.org/10.1038/s41598-017-12638-3 DB - PRIME DP - Unbound Medicine ER -