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Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde.
J AOAC Int 2018; 101(3):805-809JA

Abstract

American cranberry (Vaccinium macrocarpon) is native to Eastern North America. Recent studies have suggested that the A-type proanthocyanidins (PACs) in cranberries are effective in preventing urinary tract infection. To meet the growing interest in the cranberry market, an accurate, reliable, and simple method to determine PAC concentration is needed. In this study, a modified method using 4-dimethylaminocinnamaldehyde to quantify total PACs in cranberry products was validated. Cranberry juice extract powder, cranberry capsules containing juice extract, and cranberry juice concentrate were used as the samples in this study. With the modified method, the calibration curves for proanthocyanidin A2 had correlation coefficients (r2) of >0.99. The recoveries of two different concentrations after spiking were 97.1 and 99.1%, and the RSDs for repeatability and reproducibility were <2.7 and <1.6%, respectively.

Authors+Show Affiliations

International Chemistry Testing, 258 Main St, Suite 202, Milford, MA 01757.International Chemistry Testing, 258 Main St, Suite 202, Milford, MA 01757.Ocean Spray, 1 Ocean Spray Dr, Lakeville, MA 02349.University of Arkansas, Department of Food Science, 214 Lakeshore Dr, Searcy, AR 72143.U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Nutrient Data Laboratory, 10300 Baltimore Ave, Beltsville, MD 20705.International Chemistry Testing, 258 Main St, Suite 202, Milford, MA 01757.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28982414

Citation

Sintara, Marsha, et al. "Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde." Journal of AOAC International, vol. 101, no. 3, 2018, pp. 805-809.
Sintara M, Li L, Cunningham DG, et al. Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde. J AOAC Int. 2018;101(3):805-809.
Sintara, M., Li, L., Cunningham, D. G., Prior, R. L., Wu, X., & Chang, T. (2018). Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde. Journal of AOAC International, 101(3), pp. 805-809. doi:10.5740/jaoacint.17-0288.
Sintara M, et al. Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde. J AOAC Int. 2018 May 1;101(3):805-809. PubMed PMID: 28982414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-Laboratory Validation for Determination of Total Soluble Proanthocyanidins in Cranberry Using 4-Dimethylaminocinnamaldehyde. AU - Sintara,Marsha, AU - Li,Lin, AU - Cunningham,David G, AU - Prior,Ronald L, AU - Wu,Xianli, AU - Chang,Tony, Y1 - 2017/10/05/ PY - 2017/10/7/pubmed PY - 2018/12/28/medline PY - 2017/10/7/entrez SP - 805 EP - 809 JF - Journal of AOAC International JO - J AOAC Int VL - 101 IS - 3 N2 - American cranberry (Vaccinium macrocarpon) is native to Eastern North America. Recent studies have suggested that the A-type proanthocyanidins (PACs) in cranberries are effective in preventing urinary tract infection. To meet the growing interest in the cranberry market, an accurate, reliable, and simple method to determine PAC concentration is needed. In this study, a modified method using 4-dimethylaminocinnamaldehyde to quantify total PACs in cranberry products was validated. Cranberry juice extract powder, cranberry capsules containing juice extract, and cranberry juice concentrate were used as the samples in this study. With the modified method, the calibration curves for proanthocyanidin A2 had correlation coefficients (r2) of >0.99. The recoveries of two different concentrations after spiking were 97.1 and 99.1%, and the RSDs for repeatability and reproducibility were <2.7 and <1.6%, respectively. SN - 1944-7922 UR - https://www.unboundmedicine.com/medline/citation/28982414/Single_Laboratory_Validation_for_Determination_of_Total_Soluble_Proanthocyanidins_in_Cranberry_Using_4_Dimethylaminocinnamaldehyde_ L2 - https://www.ingentaconnect.com/openurl?genre=article&amp;issn=1060-3271&amp;volume=101&amp;issue=3&amp;spage=805&amp;aulast=Sintara DB - PRIME DP - Unbound Medicine ER -