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The c.29T>C polymorphism of the transforming growth factor beta-1 (TGFB1) gene, bone mineral density and the occurrence of low-energy fractures in patients with inflammatory bowel disease.
Mol Biol Rep. 2017 Dec; 44(6):455-461.MB

Abstract

Gastrointestinal tract conditions are frequently associated with low bone mineral density and increased risk of fractures due to osteoporosis, the latter concerning particularly inflammatory bowel disease (IBD) patients. One of the candidate genes involved in osteoporosis is the transforming growth factor beta-1 (TGFB1) whose polymorphisms may be responsible for the development of this disease. The aim of this study was to analyse the frequency of TGFB1 polymorphic variants and determine the association between the c.29T>C TGFB1 polymorphism, and bone mineral density and fractures in IBD patients. The study subjects included 198 IBD patients [100 suffering from Crohn's disease (CD) and 98 from ulcerative colitis (UC)] and 41 healthy volunteers as a control group. Densitometric bone measurements were obtained using dual energy X-ray absorptiometry. The TGFB1 genotyping was conducted using restriction fragments length polymorphism. We conducted an analysis of genotype distribution's concordance with Hardy-Weinberg equilibrium. We found statistically significant differences in lumbar spine (L2-L4) and femoral neck BMD and T-scores between CD, UC and control subgroups. The distribution of TGFB1 polymorphic variants among CD and UC patients was concordant with Hardy-Weinberg equilibrium. There were no statistically significant differences in densitometric parameters (lumbar spine and femoral neck BMD, T-score, and Z-score) between carriers of different TGFB1 polymorphisms among IBD (CD and UC) patients nor among controls. We have found no statistically significant differences in the prevalence of low-energy fractures between groups of different TGFB1 polymorphic variant carriers. The allele dose effect, recessive effect and dominant effect analysis did not show an association between low-energy fractures and the TGFB1 polymorphisms among CD and UC patients. We have not observed an association between the c.29T>C TGFB1 polymorphic variant and the bone mineral density within the cancellous and cortical bones (L2-L4 and femoral neck, respectively), or the occurrence of fractures among the IBD patients and their family members.

Authors+Show Affiliations

Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences, Przybyszewskiego Street 49, 60-355, Poznan, Poland. krela@op.pl.Department of Computer Science and Statistics, University of Medical Sciences, Poznan, Poland.Department of Family Medicine, University of Medical Sciences, Poznan, Poland.Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences, Przybyszewskiego Street 49, 60-355, Poznan, Poland.Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences, Przybyszewskiego Street 49, 60-355, Poznan, Poland.Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences, Przybyszewskiego Street 49, 60-355, Poznan, Poland.Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.Institute of Natural Fibres and Medicinal Plants, Poznan, Poland.Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences, Przybyszewskiego Street 49, 60-355, Poznan, Poland.Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28993955

Citation

Krela-Kaźmierczak, I, et al. "The c.29T>C Polymorphism of the Transforming Growth Factor Beta-1 (TGFB1) Gene, Bone Mineral Density and the Occurrence of Low-energy Fractures in Patients With Inflammatory Bowel Disease." Molecular Biology Reports, vol. 44, no. 6, 2017, pp. 455-461.
Krela-Kaźmierczak I, Michalak M, Wawrzyniak A, et al. The c.29T>C polymorphism of the transforming growth factor beta-1 (TGFB1) gene, bone mineral density and the occurrence of low-energy fractures in patients with inflammatory bowel disease. Mol Biol Rep. 2017;44(6):455-461.
Krela-Kaźmierczak, I., Michalak, M., Wawrzyniak, A., Szymczak, A., Eder, P., Łykowska-Szuber, L., Kaczmarek-Ryś, M., Drwęska-Matelska, N., Skrzypczak-Zielińska, M., Linke, K., & Słomski, R. (2017). The c.29T>C polymorphism of the transforming growth factor beta-1 (TGFB1) gene, bone mineral density and the occurrence of low-energy fractures in patients with inflammatory bowel disease. Molecular Biology Reports, 44(6), 455-461. https://doi.org/10.1007/s11033-017-4131-2
Krela-Kaźmierczak I, et al. The c.29T>C Polymorphism of the Transforming Growth Factor Beta-1 (TGFB1) Gene, Bone Mineral Density and the Occurrence of Low-energy Fractures in Patients With Inflammatory Bowel Disease. Mol Biol Rep. 2017;44(6):455-461. PubMed PMID: 28993955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The c.29T>C polymorphism of the transforming growth factor beta-1 (TGFB1) gene, bone mineral density and the occurrence of low-energy fractures in patients with inflammatory bowel disease. AU - Krela-Kaźmierczak,I, AU - Michalak,M, AU - Wawrzyniak,A, AU - Szymczak,A, AU - Eder,P, AU - Łykowska-Szuber,L, AU - Kaczmarek-Ryś,M, AU - Drwęska-Matelska,N, AU - Skrzypczak-Zielińska,M, AU - Linke,K, AU - Słomski,R, Y1 - 2017/10/09/ PY - 2016/10/24/received PY - 2017/09/19/accepted PY - 2017/10/11/pubmed PY - 2018/1/3/medline PY - 2017/10/11/entrez KW - Inflammatory bowel disease KW - Secondary osteoporosis KW - TGFB1 genetic polymorphism SP - 455 EP - 461 JF - Molecular biology reports JO - Mol Biol Rep VL - 44 IS - 6 N2 - Gastrointestinal tract conditions are frequently associated with low bone mineral density and increased risk of fractures due to osteoporosis, the latter concerning particularly inflammatory bowel disease (IBD) patients. One of the candidate genes involved in osteoporosis is the transforming growth factor beta-1 (TGFB1) whose polymorphisms may be responsible for the development of this disease. The aim of this study was to analyse the frequency of TGFB1 polymorphic variants and determine the association between the c.29T>C TGFB1 polymorphism, and bone mineral density and fractures in IBD patients. The study subjects included 198 IBD patients [100 suffering from Crohn's disease (CD) and 98 from ulcerative colitis (UC)] and 41 healthy volunteers as a control group. Densitometric bone measurements were obtained using dual energy X-ray absorptiometry. The TGFB1 genotyping was conducted using restriction fragments length polymorphism. We conducted an analysis of genotype distribution's concordance with Hardy-Weinberg equilibrium. We found statistically significant differences in lumbar spine (L2-L4) and femoral neck BMD and T-scores between CD, UC and control subgroups. The distribution of TGFB1 polymorphic variants among CD and UC patients was concordant with Hardy-Weinberg equilibrium. There were no statistically significant differences in densitometric parameters (lumbar spine and femoral neck BMD, T-score, and Z-score) between carriers of different TGFB1 polymorphisms among IBD (CD and UC) patients nor among controls. We have found no statistically significant differences in the prevalence of low-energy fractures between groups of different TGFB1 polymorphic variant carriers. The allele dose effect, recessive effect and dominant effect analysis did not show an association between low-energy fractures and the TGFB1 polymorphisms among CD and UC patients. We have not observed an association between the c.29T>C TGFB1 polymorphic variant and the bone mineral density within the cancellous and cortical bones (L2-L4 and femoral neck, respectively), or the occurrence of fractures among the IBD patients and their family members. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/28993955/The_c_29T>C_polymorphism_of_the_transforming_growth_factor_beta_1__TGFB1__gene_bone_mineral_density_and_the_occurrence_of_low_energy_fractures_in_patients_with_inflammatory_bowel_disease_ L2 - https://doi.org/10.1007/s11033-017-4131-2 DB - PRIME DP - Unbound Medicine ER -