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Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients.
Nutrients 2017; 9(10)N

Abstract

This study aims to investigate the biological activities related to hair loss of Equisetum debile extracts, including 5α-reductase inhibition, interleukin-6 (IL-6) secretion reduction, and anti-oxidation. E. debile extracts were obtained by maceration in various solvents. Crude extract (CE) was obtained by maceration in 95% ethanol. Chlorophyll-free extract (CF) was the CE which of the chlorophyll has been removed by electrocoagulation. Hexane extract (HE), ethyl acetate extract (EA), and ethanolic extract (ET) were fraction extracts obtained from maceration in hexane, ethyl acetate, and 95% ethanol, respectively. The extracts were investigated for inhibitory activity against 5α-reductase and IL-6 secretion. Total phenolic contents (TPC) were investigated and antioxidant activities were determined by means of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The inhibition of lipid peroxidation was determined by the ferric thiocyanate method. The cytotoxicity of the extracts on dermal papilla cells and irritation test by hen's egg test chorioallantoic membrane assay were also investigated. All extracts could inhibit 5α-reductase and decrease IL-6 secretion in lipopolysaccharide-stimulated macrophage. The antioxidant activity of E. debile extracts was directly related to their TPC. ET which contained the highest TPC (68.8 ± 6.7 mg GA/g) showed the highest equivalent concentration (EC₁) of 289.1 ± 26.4 mM FeSO₄/g, TEAC of 156.6 ± 34.6 mM Trolox/g, and 20.0 ± 6.0% DPPH inhibition. However, EA exhibited the highest inhibition against lipid peroxidation (57.2 ± 0.4%). In addition, EA showed no cytotoxicity on dermal papilla cell line and no irritation on chorioallantoic membrane of hen's eggs. In conclusion, EA was suggested as the most attractive ingredients for functional food and nutraceuticals because of the high inhibitory activity against 5α-reductase, IL-6 secretion, and lipid peroxidation inhibition.

Authors+Show Affiliations

Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. wantida.chaiyana@gmail.com.Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. Chanew010@gmail.com.Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. suvimol_ampoo@hotmail.com.Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. pimleelaporn@gmail.com.Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand. k_ingkaninan@yahoo.com.Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand. netiw@nu.ac.th.Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand. jukkarint@hotmail.com.School of Cosmetic Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand. nthitipramote@hotmail.com.Department of Thai Traditional Medicine, Sirindhorn College of Public Health, Phitsanulok 65130, Thailand. wisuitiprot@hotmail.com.Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, Vienna 1090, Austria. roswitha.schuster@univie.ac.at.Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, Vienna 1090, Austria. helmut.viernstein@univie.ac.at.Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, Vienna 1090, Austria. monika.mueller@univie.ac.at.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28994714

Citation

Chaiyana, Wantida, et al. "Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum Debile Roxb. Ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients." Nutrients, vol. 9, no. 10, 2017.
Chaiyana W, Punyoyai C, Somwongin S, et al. Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients. Nutrients. 2017;9(10).
Chaiyana, W., Punyoyai, C., Somwongin, S., Leelapornpisid, P., Ingkaninan, K., Waranuch, N., ... Mueller, M. (2017). Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients. Nutrients, 9(10), doi:10.3390/nu9101105.
Chaiyana W, et al. Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum Debile Roxb. Ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients. Nutrients. 2017 Oct 10;9(10) PubMed PMID: 28994714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients. AU - Chaiyana,Wantida, AU - Punyoyai,Chanun, AU - Somwongin,Suvimol, AU - Leelapornpisid,Pimporn, AU - Ingkaninan,Kornkanok, AU - Waranuch,Neti, AU - Srivilai,Jukkarin, AU - Thitipramote,Natthawut, AU - Wisuitiprot,Wudtichai, AU - Schuster,Roswitha, AU - Viernstein,Helmut, AU - Mueller,Monika, Y1 - 2017/10/10/ PY - 2017/08/27/received PY - 2017/09/21/revised PY - 2017/10/03/accepted PY - 2017/10/11/entrez PY - 2017/10/11/pubmed PY - 2018/6/6/medline KW - 5α-reductase KW - Equisetum debile KW - chorioallantoic membrane assay KW - interleukin-6 KW - lipid peroxidation JF - Nutrients JO - Nutrients VL - 9 IS - 10 N2 - This study aims to investigate the biological activities related to hair loss of Equisetum debile extracts, including 5α-reductase inhibition, interleukin-6 (IL-6) secretion reduction, and anti-oxidation. E. debile extracts were obtained by maceration in various solvents. Crude extract (CE) was obtained by maceration in 95% ethanol. Chlorophyll-free extract (CF) was the CE which of the chlorophyll has been removed by electrocoagulation. Hexane extract (HE), ethyl acetate extract (EA), and ethanolic extract (ET) were fraction extracts obtained from maceration in hexane, ethyl acetate, and 95% ethanol, respectively. The extracts were investigated for inhibitory activity against 5α-reductase and IL-6 secretion. Total phenolic contents (TPC) were investigated and antioxidant activities were determined by means of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The inhibition of lipid peroxidation was determined by the ferric thiocyanate method. The cytotoxicity of the extracts on dermal papilla cells and irritation test by hen's egg test chorioallantoic membrane assay were also investigated. All extracts could inhibit 5α-reductase and decrease IL-6 secretion in lipopolysaccharide-stimulated macrophage. The antioxidant activity of E. debile extracts was directly related to their TPC. ET which contained the highest TPC (68.8 ± 6.7 mg GA/g) showed the highest equivalent concentration (EC₁) of 289.1 ± 26.4 mM FeSO₄/g, TEAC of 156.6 ± 34.6 mM Trolox/g, and 20.0 ± 6.0% DPPH inhibition. However, EA exhibited the highest inhibition against lipid peroxidation (57.2 ± 0.4%). In addition, EA showed no cytotoxicity on dermal papilla cell line and no irritation on chorioallantoic membrane of hen's eggs. In conclusion, EA was suggested as the most attractive ingredients for functional food and nutraceuticals because of the high inhibitory activity against 5α-reductase, IL-6 secretion, and lipid peroxidation inhibition. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/28994714/Inhibition_of_5α_Reductase_IL_6_Secretion_and_Oxidation_Process_of_Equisetum_debile_Roxb__ex_Vaucher_Extract_as_Functional_Food_and_Nutraceuticals_Ingredients_ L2 - http://www.mdpi.com/resolver?pii=nu9101105 DB - PRIME DP - Unbound Medicine ER -