Tags

Type your tag names separated by a space and hit enter

Dietary cholesterol and/or n-3 fatty acid modulate delta 9-desaturase activity in rat liver microsomes.
Biochim Biophys Acta. 1988 Oct 14; 962(3):330-6.BB

Abstract

delta 9-Desaturase activity and fatty acid composition of liver microsomal phospholipids in rats fed diets enriched with either saturated (hydrogenated beef tallow) or alpha-linolenic (linseed oil) or eicosapentaenoic and docosahexaenoic (fish oil) acids with or without 2% cholesterol supplementation were investigated. Both the linseed oil and the fish oil diets inhibited delta 9-desaturase activity in the rat liver microsomes. The inhibition was greater when feeding fish oil (90%) compared with the linseed oil (60%) diet. Dietary cholesterol feeding accelerated conversion of palmitic (16:0) to palmitoleic (16:1) acid, irrespective of the fatty acid supplement. Feeding the linseed oil diet decreased, while feeding the fish oil diet increased synthesis of the monounsaturated fatty acids of n-7 series (palmitoleic and vaccenic acid) and decreased 18:1(n-9) in microsomal membrane lipids when compared with animals fed beef tallow. Addition of 2% cholesterol to the otherwise low cholesterol diets led to accumulation of 16:1(n-7), and 18:1(n-9) in microsomal membranes. These results suggest that delta 9-desaturase activity is dependent on the cholesterol contents as well as the n-3 fatty acid content of microsomal membranes on which it is localized.

Authors+Show Affiliations

Department of Foods and Nutrition, Faculty of Home Economics, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2901857

Citation

Garg, M L., et al. "Dietary Cholesterol And/or N-3 Fatty Acid Modulate Delta 9-desaturase Activity in Rat Liver Microsomes." Biochimica Et Biophysica Acta, vol. 962, no. 3, 1988, pp. 330-6.
Garg ML, Wierzbicki AA, Thomson AB, et al. Dietary cholesterol and/or n-3 fatty acid modulate delta 9-desaturase activity in rat liver microsomes. Biochim Biophys Acta. 1988;962(3):330-6.
Garg, M. L., Wierzbicki, A. A., Thomson, A. B., & Clandinin, M. T. (1988). Dietary cholesterol and/or n-3 fatty acid modulate delta 9-desaturase activity in rat liver microsomes. Biochimica Et Biophysica Acta, 962(3), 330-6.
Garg ML, et al. Dietary Cholesterol And/or N-3 Fatty Acid Modulate Delta 9-desaturase Activity in Rat Liver Microsomes. Biochim Biophys Acta. 1988 Oct 14;962(3):330-6. PubMed PMID: 2901857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary cholesterol and/or n-3 fatty acid modulate delta 9-desaturase activity in rat liver microsomes. AU - Garg,M L, AU - Wierzbicki,A A, AU - Thomson,A B, AU - Clandinin,M T, PY - 1988/10/14/pubmed PY - 1988/10/14/medline PY - 1988/10/14/entrez SP - 330 EP - 6 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 962 IS - 3 N2 - delta 9-Desaturase activity and fatty acid composition of liver microsomal phospholipids in rats fed diets enriched with either saturated (hydrogenated beef tallow) or alpha-linolenic (linseed oil) or eicosapentaenoic and docosahexaenoic (fish oil) acids with or without 2% cholesterol supplementation were investigated. Both the linseed oil and the fish oil diets inhibited delta 9-desaturase activity in the rat liver microsomes. The inhibition was greater when feeding fish oil (90%) compared with the linseed oil (60%) diet. Dietary cholesterol feeding accelerated conversion of palmitic (16:0) to palmitoleic (16:1) acid, irrespective of the fatty acid supplement. Feeding the linseed oil diet decreased, while feeding the fish oil diet increased synthesis of the monounsaturated fatty acids of n-7 series (palmitoleic and vaccenic acid) and decreased 18:1(n-9) in microsomal membrane lipids when compared with animals fed beef tallow. Addition of 2% cholesterol to the otherwise low cholesterol diets led to accumulation of 16:1(n-7), and 18:1(n-9) in microsomal membranes. These results suggest that delta 9-desaturase activity is dependent on the cholesterol contents as well as the n-3 fatty acid content of microsomal membranes on which it is localized. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/2901857/Dietary_cholesterol_and/or_n_3_fatty_acid_modulate_delta_9_desaturase_activity_in_rat_liver_microsomes_ DB - PRIME DP - Unbound Medicine ER -