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Human macrophages differentiated in the presence of vitamin D3 restrict dengue virus infection and innate responses by downregulating mannose receptor expression.

Abstract

BACKGROUND

Severe dengue disease is associated with high viral loads and overproduction of pro-inflammatory cytokines, suggesting impairment in the control of dengue virus (DENV) and the mechanisms that regulate cytokine production. Vitamin D3 has been described as an important modulator of immune responses to several pathogens. Interestingly, increasing evidence has associated vitamin D with decreased DENV infection and early disease recovery, yet the molecular mechanisms whereby vitamin D reduces DENV infection are not well understood.

METHODS AND PRINCIPAL FINDINGS

Macrophages represent important cell targets for DENV replication and consequently, they are key drivers of dengue disease. In this study we evaluated the effect of vitamin D3 on the differentiation of monocyte-derived macrophages (MDM) and their susceptibility and cytokine response to DENV. Our data demonstrate that MDM differentiated in the presence of vitamin D3 (D3-MDM) restrict DENV infection and moderate the classical inflammatory cytokine response. Mechanistically, vitamin D3-driven differentiation led to reduced surface expression of C-type lectins including the mannose receptor (MR, CD206) that is known to act as primary receptor for DENV attachment on macrophages and to trigger of immune signaling. Consequently, DENV bound less efficiently to vitamin D3-differentiated macrophages, leading to lower infection. Interestingly, IL-4 enhanced infection was reduced in D3-MDM by restriction of MR expression. Moreover, we detected moderate secretion of TNF-α, IL-1β, and IL-10 in D3-MDM, likely due to less MR engagement during DENV infection.

CONCLUSIONS/SIGNIFICANCE

Our findings reveal a molecular mechanism by which vitamin D counteracts DENV infection and progression of severe disease, and indicates its potential relevance as a preventive or therapeutic candidate.

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  • Authors+Show Affiliations

    ,

    Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia. Department of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

    ,

    Department of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

    ,

    Infettare, Facultad de Medicina, Universidad Cooperativa de Colombia, Medellín, Colombia.

    ,

    Department of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

    Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.

    Source

    PLoS neglected tropical diseases 11:10 2017 Oct pg e0005904

    MeSH

    Adult
    Animals
    Blood Donors
    Cell Differentiation
    Cell Line
    Cholecalciferol
    Culicidae
    Dengue Virus
    Down-Regulation
    Female
    Gene Expression Regulation
    Humans
    Immunity, Innate
    Lectins, C-Type
    Macrophages
    Male
    Mannose-Binding Lectins
    Receptors, Cell Surface
    Virus Replication

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29020083