Tags

Type your tag names separated by a space and hit enter

Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway.
Daru. 2017 Oct 11; 25(1):21.DARU

Abstract

BACKGROUND

Heqi San, a traditional Chinese medicine (TCM) has been reported to regulate hormone levels in patients with metabolic disease, suggesting a potential clinical application. In the current study, we aimed to elucidate the effect of Heqi San on rat model of polycystic ovary syndrome (PCOS).

METHOD

PCOS model was established in female SD rats. Rats were randomly divided into four groups: the control, untreated PCOS model, Heqi San treated PCOS model (8.1 g/kg) and metformin (MET) treated PCOS model (135 mg/kg) groups. All animals were subcutaneously injected with 6 mg/100 g dehydroepiandrosterone (DHEA) in the neck once a day for 20 consecutive days. The serum hormone levels were measured by ELISA. The ovarian tissues were stained with hematoxylin and eosin (HE) to undergo pathological examination. The expression levels of GLTU4 and PTEN mRNA were examined by real time PCR. The crucial proteins in the PI3K/APT pathway were analyzed by western blotting. Then, the functions of the target genes were analyzed using bioinformatics approaches.

RESULTS

We found that Heqi San was able to recover the serum hormone levels and improve insulin resistance in PCOS rat model. A morphological lesion of the ovary was also restored with the Heqi San treatment. More importantly, we discovered a correlation between the PI3K/AKT signaling pathway and the beneficial effects of Heqi San, demonstrating that its application could alter the expression levels of p-ERK, p-AKT, p-GSK3β, IRS-1, PTEN and GLTU4, all key factors in the PI3K/APT pathway. Through a bioinformatical analysis, we predicted the related gene function and pathway of the pathological mechanism of PCOS and found miRNAs that are likely to be critical in PCOS occurrence, including rno-miR-144-3p, rno-miR-30c-2-3p, rno-miR-486, rno-miR-3586-3p and rno-miR-146b-5p.

CONCLUSION

The beneficial effects of Heqi on PCOS, including alter serum hormone levels, recover ovary morphological lesions and improve insulin resistance, which is mediated through the PI3K/AKT pathway. The potential role of miRNA-144-3p in PCOS pathogenesis.

Authors+Show Affiliations

Department of Endocrine, Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong, 518033, China.Department of Endocrine, Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong, 518033, China. 369377040@qq.com.Longhua Central Hospital of Shenzhen, Shenzhen, Guangdong, 518000, China.Department of Endocrine, Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong, 518033, China.Department of Endocrine, Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong, 518033, China.Department of Endocrine, Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong, 518033, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29020999

Citation

Zhao, Hengxia, et al. "Beneficial Effects of Heqi San On Rat Model of Polycystic Ovary Syndrome Through the PI3K/AKT Pathway." Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences, vol. 25, no. 1, 2017, p. 21.
Zhao H, Zhou D, Chen Y, et al. Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway. Daru. 2017;25(1):21.
Zhao, H., Zhou, D., Chen, Y., Liu, D., Chu, S., & Zhang, S. (2017). Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway. Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 25(1), 21. https://doi.org/10.1186/s40199-017-0188-7
Zhao H, et al. Beneficial Effects of Heqi San On Rat Model of Polycystic Ovary Syndrome Through the PI3K/AKT Pathway. Daru. 2017 Oct 11;25(1):21. PubMed PMID: 29020999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway. AU - Zhao,Hengxia, AU - Zhou,Daocheng, AU - Chen,Ye, AU - Liu,Deliang, AU - Chu,Shufang, AU - Zhang,Shimao, Y1 - 2017/10/11/ PY - 2017/06/07/received PY - 2017/10/02/accepted PY - 2017/10/13/entrez PY - 2017/10/13/pubmed PY - 2018/7/24/medline KW - Heqi san KW - PI3K/AKT pathway KW - Polycystic ovary syndrome KW - miRNA PTEN SP - 21 EP - 21 JF - Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences JO - Daru VL - 25 IS - 1 N2 - BACKGROUND: Heqi San, a traditional Chinese medicine (TCM) has been reported to regulate hormone levels in patients with metabolic disease, suggesting a potential clinical application. In the current study, we aimed to elucidate the effect of Heqi San on rat model of polycystic ovary syndrome (PCOS). METHOD: PCOS model was established in female SD rats. Rats were randomly divided into four groups: the control, untreated PCOS model, Heqi San treated PCOS model (8.1 g/kg) and metformin (MET) treated PCOS model (135 mg/kg) groups. All animals were subcutaneously injected with 6 mg/100 g dehydroepiandrosterone (DHEA) in the neck once a day for 20 consecutive days. The serum hormone levels were measured by ELISA. The ovarian tissues were stained with hematoxylin and eosin (HE) to undergo pathological examination. The expression levels of GLTU4 and PTEN mRNA were examined by real time PCR. The crucial proteins in the PI3K/APT pathway were analyzed by western blotting. Then, the functions of the target genes were analyzed using bioinformatics approaches. RESULTS: We found that Heqi San was able to recover the serum hormone levels and improve insulin resistance in PCOS rat model. A morphological lesion of the ovary was also restored with the Heqi San treatment. More importantly, we discovered a correlation between the PI3K/AKT signaling pathway and the beneficial effects of Heqi San, demonstrating that its application could alter the expression levels of p-ERK, p-AKT, p-GSK3β, IRS-1, PTEN and GLTU4, all key factors in the PI3K/APT pathway. Through a bioinformatical analysis, we predicted the related gene function and pathway of the pathological mechanism of PCOS and found miRNAs that are likely to be critical in PCOS occurrence, including rno-miR-144-3p, rno-miR-30c-2-3p, rno-miR-486, rno-miR-3586-3p and rno-miR-146b-5p. CONCLUSION: The beneficial effects of Heqi on PCOS, including alter serum hormone levels, recover ovary morphological lesions and improve insulin resistance, which is mediated through the PI3K/AKT pathway. The potential role of miRNA-144-3p in PCOS pathogenesis. SN - 2008-2231 UR - https://www.unboundmedicine.com/medline/citation/29020999/Beneficial_effects_of_Heqi_san_on_rat_model_of_polycystic_ovary_syndrome_through_the_PI3K/AKT_pathway_ L2 - https://darujps.biomedcentral.com/articles/10.1186/s40199-017-0188-7 DB - PRIME DP - Unbound Medicine ER -