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Concomitant increase of somatostatin, neuropeptide Y and glutamate decarboxylase in the frontal cortex of rats with decreased seizure threshold.
Neuroscience. 1988 Aug; 26(2):379-85.N

Abstract

The neuropeptides somatostatin and neuropeptide Y and the activity of glutamate decarboxylase were determined in the frontal cortex of rats subjected to experimental epilepsy. Two different animal models, (1) rats kindled for 4 weeks by daily injection of pentylenetetrazole, and (2) rats which had undergone strong limbic seizures induced by kainic acid, were used. Decreased seizure threshold, as shown by injection of a subconvulsive dose of pentylenetetrazole, was observed 10 days after the last kindling session and 1 month after injection of kainic acid, respectively. Significantly increased levels of somatostatin (by 60%), neuropeptide Y (135%) and increased activity of glutamate decarboxylase (22%) were found in the frontal cortex of rats previously treated with kainic acid. Separation of somatostatin-like immunoreactivity by size exclusion high-performance liquid chromatography showed a marked increase of immunoreactivity in fractions containing the somatostatin precursor (by 200%) and less prominently of somatostatin-14 and somatostatin-28 (by 60 and 80%, respectively). Michaelis-Menten kinetics of glutamate decarboxylase revealed an increased maximal velocity (Vmax) in the frontal cortex of kainic acid-treated rats, but no change in the Km value was found. Similar results were also obtained in pentylenetetrazole-kindled rats. Injection of cysteamine (100 mg/kg, i.p.) resulting in a 30% decrease of cortical somatostatin in kainic acid-pretreated rats markedly suppressed seizures induced by an otherwise subconvulsive dose of pentylenetetrazole.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Marksteiner J
Department of Pharmacology, University of Innsbruck, Austria.
Sperk G
No affiliation info available

MeSH

AnimalsCysteamineDose-Response Relationship, DrugEpilepsyFrontal LobeGlutamate DecarboxylaseKainic AcidKindling, NeurologicMaleNeuropeptide YPentylenetetrazoleRatsRats, Inbred StrainsSomatostatinTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2902535

Citation

Marksteiner, J, and G Sperk. "Concomitant Increase of Somatostatin, Neuropeptide Y and Glutamate Decarboxylase in the Frontal Cortex of Rats With Decreased Seizure Threshold." Neuroscience, vol. 26, no. 2, 1988, pp. 379-85.
Marksteiner J, Sperk G. Concomitant increase of somatostatin, neuropeptide Y and glutamate decarboxylase in the frontal cortex of rats with decreased seizure threshold. Neuroscience. 1988;26(2):379-85.
Marksteiner, J., & Sperk, G. (1988). Concomitant increase of somatostatin, neuropeptide Y and glutamate decarboxylase in the frontal cortex of rats with decreased seizure threshold. Neuroscience, 26(2), 379-85.
Marksteiner J, Sperk G. Concomitant Increase of Somatostatin, Neuropeptide Y and Glutamate Decarboxylase in the Frontal Cortex of Rats With Decreased Seizure Threshold. Neuroscience. 1988;26(2):379-85. PubMed PMID: 2902535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Concomitant increase of somatostatin, neuropeptide Y and glutamate decarboxylase in the frontal cortex of rats with decreased seizure threshold. AU - Marksteiner,J, AU - Sperk,G, PY - 1988/8/1/pubmed PY - 1988/8/1/medline PY - 1988/8/1/entrez SP - 379 EP - 85 JF - Neuroscience JO - Neuroscience VL - 26 IS - 2 N2 - The neuropeptides somatostatin and neuropeptide Y and the activity of glutamate decarboxylase were determined in the frontal cortex of rats subjected to experimental epilepsy. Two different animal models, (1) rats kindled for 4 weeks by daily injection of pentylenetetrazole, and (2) rats which had undergone strong limbic seizures induced by kainic acid, were used. Decreased seizure threshold, as shown by injection of a subconvulsive dose of pentylenetetrazole, was observed 10 days after the last kindling session and 1 month after injection of kainic acid, respectively. Significantly increased levels of somatostatin (by 60%), neuropeptide Y (135%) and increased activity of glutamate decarboxylase (22%) were found in the frontal cortex of rats previously treated with kainic acid. Separation of somatostatin-like immunoreactivity by size exclusion high-performance liquid chromatography showed a marked increase of immunoreactivity in fractions containing the somatostatin precursor (by 200%) and less prominently of somatostatin-14 and somatostatin-28 (by 60 and 80%, respectively). Michaelis-Menten kinetics of glutamate decarboxylase revealed an increased maximal velocity (Vmax) in the frontal cortex of kainic acid-treated rats, but no change in the Km value was found. Similar results were also obtained in pentylenetetrazole-kindled rats. Injection of cysteamine (100 mg/kg, i.p.) resulting in a 30% decrease of cortical somatostatin in kainic acid-pretreated rats markedly suppressed seizures induced by an otherwise subconvulsive dose of pentylenetetrazole.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/2902535/Concomitant_increase_of_somatostatin_neuropeptide_Y_and_glutamate_decarboxylase_in_the_frontal_cortex_of_rats_with_decreased_seizure_threshold_ DB - PRIME DP - Unbound Medicine ER -