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Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring.
Nutrients. 2017 Oct 13; 9(10)N

Abstract

Maternal nutrition during gestation is involved in the offspring's intestinal development and immunity. The aim of this study was to (1) determine the effects of maternal energy on intestinal digestion and absorption function in offspring, using pigs as a model; and (2) to evaluate the potential effect and mechanisms of maternal energy in modulating immune responses of lipopolysaccharide (LPS)-challenged piglets. After mating, thirty-six nine-parity sows (Landrace × Yorkshire), body weight (BW) (initial body weight 233.56 ± 2.77 kg) were allocated to two dietary treatment groups; a control diet (CON) group and a low-energy diet (LED) group. The nutrient levels of the CON were based on the nutrient recommendations by the National Research Council (NRC, 2012), and contained 3.40 MCal digestible energy (DE)/kg diet and 7.3% crude protein; while the LED contained 3.00 MCal DE/kg diet. The dietary treatments were introduced from day 1 of gestation to farrowing. Intestine samples were collected from the pigs' offspring at birth, and at weaning (day 28 post-birth). At weaning, male pigs from control and LED groups were intraperitoneally injected with LPS (50 μg/kg body weight) or saline (n = 6), and sacrificed at 4 h post-injection to collect blood, intestine and digesta samples for biochemical analysis. The results indicated that the maternal LED markedly decreased the BW, small intestinal weight, and the ratio of jejunum and ileum villus height to crypt depth in the offspring. Moreover, the activities of lactase and sucrase in newborn piglets' intestine, and sucrase and maltase in weaning piglet intestine were markedly decreased by the maternal LED. In addition, maternal LED significantly increased the mRNA relative expression of ileal IL-6 and TNF-α in newborn piglets. Plasma IL-1β concentration and colonic Escherichia coli amount were affected by maternal diet (p < 0.05) and LPS challenge (p < 0.001). Maternal LED significant increased the mRNA relative expression of ileal TLR-4, IL-1β and NF-κB as well as decreased ZO-1 in weaning pigs after LPS challenge (p < 0.05). In conclusion, decreasing energy intake could suppress the offspring's intestinal digestion and absorption function, and increase the susceptibility of weaning piglets to LPS challenge.

Authors+Show Affiliations

Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. chenyuling1994@foxmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. mdl19920101@foxmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. 18728158035@139.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. CY199404@foxmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. clianqiang@hotmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. fangzhengfeng@hotmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. shengyu_x@hotmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. able588@163.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. fengb123d@163.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. lijian522@hotmail.com.Institute of Animal Nutrition, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu 611130, China. sow_nutrition@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29027951

Citation

Chen, Yuling, et al. "Effects of Maternal Low-Energy Diet During Gestation On Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring." Nutrients, vol. 9, no. 10, 2017.
Chen Y, Mou D, Hu L, et al. Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring. Nutrients. 2017;9(10).
Chen, Y., Mou, D., Hu, L., Zhen, J., Che, L., Fang, Z., Xu, S., Lin, Y., Feng, B., Li, J., & Wu, . (2017). Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring. Nutrients, 9(10). https://doi.org/10.3390/nu9101115
Chen Y, et al. Effects of Maternal Low-Energy Diet During Gestation On Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring. Nutrients. 2017 Oct 13;9(10) PubMed PMID: 29027951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring. AU - Chen,Yuling, AU - Mou,Daolin, AU - Hu,Liang, AU - Zhen,Jie, AU - Che,Lianqiang, AU - Fang,Zhengfeng, AU - Xu,Shengyu, AU - Lin,Yan, AU - Feng,Bin, AU - Li,Jian, AU - Wu,De, Y1 - 2017/10/13/ PY - 2017/08/05/received PY - 2017/09/21/revised PY - 2017/10/03/accepted PY - 2017/10/14/entrez PY - 2017/10/14/pubmed PY - 2018/6/6/medline KW - immunity KW - intestinal KW - low energy diet KW - maternal KW - offspring JF - Nutrients JO - Nutrients VL - 9 IS - 10 N2 - Maternal nutrition during gestation is involved in the offspring's intestinal development and immunity. The aim of this study was to (1) determine the effects of maternal energy on intestinal digestion and absorption function in offspring, using pigs as a model; and (2) to evaluate the potential effect and mechanisms of maternal energy in modulating immune responses of lipopolysaccharide (LPS)-challenged piglets. After mating, thirty-six nine-parity sows (Landrace × Yorkshire), body weight (BW) (initial body weight 233.56 ± 2.77 kg) were allocated to two dietary treatment groups; a control diet (CON) group and a low-energy diet (LED) group. The nutrient levels of the CON were based on the nutrient recommendations by the National Research Council (NRC, 2012), and contained 3.40 MCal digestible energy (DE)/kg diet and 7.3% crude protein; while the LED contained 3.00 MCal DE/kg diet. The dietary treatments were introduced from day 1 of gestation to farrowing. Intestine samples were collected from the pigs' offspring at birth, and at weaning (day 28 post-birth). At weaning, male pigs from control and LED groups were intraperitoneally injected with LPS (50 μg/kg body weight) or saline (n = 6), and sacrificed at 4 h post-injection to collect blood, intestine and digesta samples for biochemical analysis. The results indicated that the maternal LED markedly decreased the BW, small intestinal weight, and the ratio of jejunum and ileum villus height to crypt depth in the offspring. Moreover, the activities of lactase and sucrase in newborn piglets' intestine, and sucrase and maltase in weaning piglet intestine were markedly decreased by the maternal LED. In addition, maternal LED significantly increased the mRNA relative expression of ileal IL-6 and TNF-α in newborn piglets. Plasma IL-1β concentration and colonic Escherichia coli amount were affected by maternal diet (p < 0.05) and LPS challenge (p < 0.001). Maternal LED significant increased the mRNA relative expression of ileal TLR-4, IL-1β and NF-κB as well as decreased ZO-1 in weaning pigs after LPS challenge (p < 0.05). In conclusion, decreasing energy intake could suppress the offspring's intestinal digestion and absorption function, and increase the susceptibility of weaning piglets to LPS challenge. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/29027951/Effects_of_Maternal_Low_Energy_Diet_during_Gestation_on_Intestinal_Morphology_Disaccharidase_Activity_and_Immune_Response_to_Lipopolysaccharide_Challenge_in_Pig_Offspring_ L2 - http://www.mdpi.com/resolver?pii=nu9101115 DB - PRIME DP - Unbound Medicine ER -