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Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent In Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight.
Toxicol Sci. 2018 01 01; 161(1):125-138.TS

Abstract

In this study, rapid one step facile synthesis of silver nanoparticles (AgNPs) was done using culture supernatant of two Gram positive (B. thuringiensis and S. aureus) and Gram negative (E. coli and Salmonella typhimurium [STAgNP]) bacterial strains and were termed as "Bacillus thuringiensis," "Staphylococcus aureus," "Escherichia coli," and "STAgNP," respectively. Synthesized AgNPs were well characterized with the help of different standard techniques like FESEM, DLS, UV-Vis spectroscopy, and Fourier transform infrared. Mechanism of AgNPs synthesis was elucidated using in silico approach. In vivo cytotoxicity of synthesized AgNPs was assessed in embryonic Zebrafish model with the help of uptake, oxidative stress, and apoptosis induction experimental assays, and the mechanism was investigated through in silico approach at the molecular level. The result showed successful biosynthesis of 20-40 nm sized AgNPs stable with zeta potential of - 45 to - 35 mV having standard silver nanoparticles SPR peaks due to the interaction of reduced silver particles with amino acid residues of bapA proteins of the bacterial supernatant. In vivo cytotoxicity with embryonic Zebrafish was found to be dependent on biogenicity and concentration of biosynthesized AgNPs as consequence of oxidative stress induction and apoptosis due to the influential regulation of sod1 and tp53 genes clarified by pathway analysis with reference to experimental and computational results. The study suggested that cytotoxicity of biologically synthesized silver nanoparticles from bacteria depends on strain specificity with significant difference in use of Gram positive and Gram negative bacterial strains.

Authors+Show Affiliations

School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.Institute of Physics, Bhubaneswar, Orissa 751005, India.School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.School of Applied Sciences, KIIT University, Bhubaneswar, Orissa 751024, India.School of Biotechnology, KIIT University, Bhubaneswar, Orissa 751024, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29029321

Citation

Verma, Suresh K., et al. "Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent in Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight." Toxicological Sciences : an Official Journal of the Society of Toxicology, vol. 161, no. 1, 2018, pp. 125-138.
Verma SK, Jha E, Panda PK, et al. Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent In Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight. Toxicol Sci. 2018;161(1):125-138.
Verma, S. K., Jha, E., Panda, P. K., Mishra, A., Thirumurugan, A., Das, B., Parashar, S. K. S., & Suar, M. (2018). Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent In Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight. Toxicological Sciences : an Official Journal of the Society of Toxicology, 161(1), 125-138. https://doi.org/10.1093/toxsci/kfx204
Verma SK, et al. Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent in Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight. Toxicol Sci. 2018 01 1;161(1):125-138. PubMed PMID: 29029321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid Novel Facile Biosynthesized Silver Nanoparticles From Bacterial Release Induce Biogenicity and Concentration Dependent In Vivo Cytotoxicity With Embryonic Zebrafish-A Mechanistic Insight. AU - Verma,Suresh K, AU - Jha,Ealisha, AU - Panda,Pritam Kumar, AU - Mishra,Anurag, AU - Thirumurugan,Arun, AU - Das,Biswadeep, AU - Parashar,S K S, AU - Suar,Mrutyunjay, PY - 2017/10/14/pubmed PY - 2019/1/29/medline PY - 2017/10/14/entrez KW - ROS KW - Zebrafish KW - apoptosis KW - biogenicity KW - silver nanoparticle KW - sod1 KW - tp53 SP - 125 EP - 138 JF - Toxicological sciences : an official journal of the Society of Toxicology JO - Toxicol Sci VL - 161 IS - 1 N2 - In this study, rapid one step facile synthesis of silver nanoparticles (AgNPs) was done using culture supernatant of two Gram positive (B. thuringiensis and S. aureus) and Gram negative (E. coli and Salmonella typhimurium [STAgNP]) bacterial strains and were termed as "Bacillus thuringiensis," "Staphylococcus aureus," "Escherichia coli," and "STAgNP," respectively. Synthesized AgNPs were well characterized with the help of different standard techniques like FESEM, DLS, UV-Vis spectroscopy, and Fourier transform infrared. Mechanism of AgNPs synthesis was elucidated using in silico approach. In vivo cytotoxicity of synthesized AgNPs was assessed in embryonic Zebrafish model with the help of uptake, oxidative stress, and apoptosis induction experimental assays, and the mechanism was investigated through in silico approach at the molecular level. The result showed successful biosynthesis of 20-40 nm sized AgNPs stable with zeta potential of - 45 to - 35 mV having standard silver nanoparticles SPR peaks due to the interaction of reduced silver particles with amino acid residues of bapA proteins of the bacterial supernatant. In vivo cytotoxicity with embryonic Zebrafish was found to be dependent on biogenicity and concentration of biosynthesized AgNPs as consequence of oxidative stress induction and apoptosis due to the influential regulation of sod1 and tp53 genes clarified by pathway analysis with reference to experimental and computational results. The study suggested that cytotoxicity of biologically synthesized silver nanoparticles from bacteria depends on strain specificity with significant difference in use of Gram positive and Gram negative bacterial strains. SN - 1096-0929 UR - https://www.unboundmedicine.com/medline/citation/29029321/Rapid_Novel_Facile_Biosynthesized_Silver_Nanoparticles_From_Bacterial_Release_Induce_Biogenicity_and_Concentration_Dependent_In_Vivo_Cytotoxicity_With_Embryonic_Zebrafish_A_Mechanistic_Insight_ L2 - https://academic.oup.com/toxsci/article-lookup/doi/10.1093/toxsci/kfx204 DB - PRIME DP - Unbound Medicine ER -