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Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis.
Biotechnol Adv. 2018 Jan - Feb; 36(1):120-131.BA

Abstract

Industrial enzymatic reactions requiring 1,4-NAD(P)H2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P)H2 from NAD(P) and recycle the cofactor for as many turnovers as possible. Renewed interest in recycling the cofactor via electrochemical means is motivated by the low cost of performing electrochemical reactions, easy monitoring of the reaction progress, and straightforward product recovery. However, electrochemical cofactor regeneration methods invariably produce adventitious reduced cofactor side products which result in unproductive loss of input NAD(P). We review various literature strategies for mitigating adventitious product formation by electrochemical cofactor regeneration systems, and offer insight as to how a successful electrochemical bioreactor system could be constructed to engineer efficient 1,4-NAD(P)H2-dependent enzyme reactions of interest to the industrial biocatalysis community.

Authors+Show Affiliations

Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States.BioChemInsights, Inc., Malvern, PA 19355, United States.BioChemInsights, Inc., Malvern, PA 19355, United States.Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Materials Science and Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States.Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States. Electronic address: koffam@rpi.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

Language

eng

PubMed ID

29030132

Citation

Morrison, Clifford S., et al. "Improved Strategies for Electrochemical 1,4-NAD(P)H2 Regeneration: a New Era of Bioreactors for Industrial Biocatalysis." Biotechnology Advances, vol. 36, no. 1, 2018, pp. 120-131.
Morrison CS, Armiger WB, Dodds DR, et al. Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis. Biotechnol Adv. 2018;36(1):120-131.
Morrison, C. S., Armiger, W. B., Dodds, D. R., Dordick, J. S., & Koffas, M. A. G. (2018). Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis. Biotechnology Advances, 36(1), 120-131. https://doi.org/10.1016/j.biotechadv.2017.10.003
Morrison CS, et al. Improved Strategies for Electrochemical 1,4-NAD(P)H2 Regeneration: a New Era of Bioreactors for Industrial Biocatalysis. Biotechnol Adv. 2018 Jan - Feb;36(1):120-131. PubMed PMID: 29030132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis. AU - Morrison,Clifford S, AU - Armiger,William B, AU - Dodds,David R, AU - Dordick,Jonathan S, AU - Koffas,Mattheos A G, Y1 - 2017/10/10/ PY - 2017/08/23/received PY - 2017/10/02/revised PY - 2017/10/06/accepted PY - 2017/10/17/pubmed PY - 2019/1/16/medline PY - 2017/10/15/entrez KW - Biocatalysis KW - Cofactor regeneration KW - Cofactors KW - Electrochemical bioreactors KW - Industrial biotechnology KW - NADH KW - NADPH KW - Renalase SP - 120 EP - 131 JF - Biotechnology advances JO - Biotechnol. Adv. VL - 36 IS - 1 N2 - Industrial enzymatic reactions requiring 1,4-NAD(P)H2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P)H2 from NAD(P) and recycle the cofactor for as many turnovers as possible. Renewed interest in recycling the cofactor via electrochemical means is motivated by the low cost of performing electrochemical reactions, easy monitoring of the reaction progress, and straightforward product recovery. However, electrochemical cofactor regeneration methods invariably produce adventitious reduced cofactor side products which result in unproductive loss of input NAD(P). We review various literature strategies for mitigating adventitious product formation by electrochemical cofactor regeneration systems, and offer insight as to how a successful electrochemical bioreactor system could be constructed to engineer efficient 1,4-NAD(P)H2-dependent enzyme reactions of interest to the industrial biocatalysis community. SN - 1873-1899 UR - https://www.unboundmedicine.com/medline/citation/29030132/Improved_strategies_for_electrochemical_14_NAD_P_H2_regeneration:_A_new_era_of_bioreactors_for_industrial_biocatalysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0734-9750(17)30123-4 DB - PRIME DP - Unbound Medicine ER -