TY - JOUR T1 - A sensitive biosensor using double-layer capillary based immunomagnetic separation and invertase-nanocluster based signal amplification for rapid detection of foodborne pathogen. AU - Huang,Fengchun, AU - Zhang,Huilin, AU - Wang,Lei, AU - Lai,Weihua, AU - Lin,Jianhan, Y1 - 2017/10/03/ PY - 2017/08/16/received PY - 2017/09/23/revised PY - 2017/10/02/accepted PY - 2017/10/17/pubmed PY - 2018/6/26/medline PY - 2017/10/17/entrez KW - Biosensor KW - Double-layer capillary KW - E. coli O157:H7 KW - Glucose meter KW - High gradient magnetic separation KW - Invertase nanoclusters SP - 583 EP - 590 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 100 N2 - Combining double-layer capillary based high gradient immunomagnetic separation, invertase-nanocluster based signal amplification and glucose meter based signal detection, a novel biosensor was developed for sensitive and rapid detection of E. coli O157:H7 in this study. The streptavidin modified magnetic nanobeads (MNBs) were conjugated with the biotinylated polyclonal antibodies against E. coli O157:H7 to form the immune MNBs, which were captured by the high gradient magnetic field in the double-layer capillary to specifically separate and efficiently concentrate the target bacteria. Calcium chloride was used with the monoclonal antibodies against E. coli O157:H7 and the invertase to form the immune invertase-nanoclusters (INCs), which were used to react with the target bacteria to form the MNB-bacteria-INC complexes in the capillary. The sucrose was then injected into the capillary and catalyzed by the invertase on the complexes into the glucose, which was detected using the glucose meter to obtain the concentration of the glucose for final determination of the E. coli O157:H7 cells in the sample. A linear relationship between the readout of the glucose meter and the concentration of the E. coli O157:H7 cells (from 102 to 107 CFU/mL) was found and the lower detection limit of this biosensor was 79 CFU/mL. This biosensor might be extended for the detection of other foodborne pathogens by changing the antibodies and has shown the potential for the detection of foodborne pathogens in a large volume of sample to further increase the sensitivity. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29032045/A_sensitive_biosensor_using_double_layer_capillary_based_immunomagnetic_separation_and_invertase_nanocluster_based_signal_amplification_for_rapid_detection_of_foodborne_pathogen_ DB - PRIME DP - Unbound Medicine ER -