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Comparison of three commercial amphetamine immunoassays for detection of methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine.
J Anal Toxicol 1988 Jul-Aug; 12(4):229-33JA

Abstract

Three commercial immunoassays for detection of amphetamines in urine, Abuscreen radioimmunoassay (RIA), enzyme-multiplied immunoassay technique (EMIT), and the TDx fluorescence polarization immunoassay (FPIA), have been investigated for detection of methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDE). Blank urine was spiked with 0.1 to 3000 micrograms/mL amphetamine analog and used as sample in the assays. With the RIA and FPIA, MDA displayed a higher cross-reactivity to amphetamine than other analogs, but with EMIT, methamphetamine was relatively similar to amphetamine while MDA, MDMA, and MDE were less reactive. The high specificity RIA and the EMIT confirmation reagents for urine amphetamines produced significant, but relatively minor, reduction in the detectability of these analogs. The variation in cross-reactivity seen between the different assays suggests that RIA, EMIT, and FPIA antibodies have different recognition sites; however, all three immunoassays do detect the illicit amphetamine analogs to varying degrees.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, University of Utah, College of Pharmacy, Salt Lake City 84112.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2903272

Citation

Ruangyuttikarn, W, and D E. Moody. "Comparison of Three Commercial Amphetamine Immunoassays for Detection of Methamphetamine, Methylenedioxyamphetamine, Methylenedioxymethamphetamine, and Methylenedioxyethylamphetamine." Journal of Analytical Toxicology, vol. 12, no. 4, 1988, pp. 229-33.
Ruangyuttikarn W, Moody DE. Comparison of three commercial amphetamine immunoassays for detection of methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine. J Anal Toxicol. 1988;12(4):229-33.
Ruangyuttikarn, W., & Moody, D. E. (1988). Comparison of three commercial amphetamine immunoassays for detection of methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine. Journal of Analytical Toxicology, 12(4), pp. 229-33.
Ruangyuttikarn W, Moody DE. Comparison of Three Commercial Amphetamine Immunoassays for Detection of Methamphetamine, Methylenedioxyamphetamine, Methylenedioxymethamphetamine, and Methylenedioxyethylamphetamine. J Anal Toxicol. 1988;12(4):229-33. PubMed PMID: 2903272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of three commercial amphetamine immunoassays for detection of methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine. AU - Ruangyuttikarn,W, AU - Moody,D E, PY - 1988/7/1/pubmed PY - 1988/7/1/medline PY - 1988/7/1/entrez SP - 229 EP - 33 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 12 IS - 4 N2 - Three commercial immunoassays for detection of amphetamines in urine, Abuscreen radioimmunoassay (RIA), enzyme-multiplied immunoassay technique (EMIT), and the TDx fluorescence polarization immunoassay (FPIA), have been investigated for detection of methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDE). Blank urine was spiked with 0.1 to 3000 micrograms/mL amphetamine analog and used as sample in the assays. With the RIA and FPIA, MDA displayed a higher cross-reactivity to amphetamine than other analogs, but with EMIT, methamphetamine was relatively similar to amphetamine while MDA, MDMA, and MDE were less reactive. The high specificity RIA and the EMIT confirmation reagents for urine amphetamines produced significant, but relatively minor, reduction in the detectability of these analogs. The variation in cross-reactivity seen between the different assays suggests that RIA, EMIT, and FPIA antibodies have different recognition sites; however, all three immunoassays do detect the illicit amphetamine analogs to varying degrees. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/2903272/Comparison_of_three_commercial_amphetamine_immunoassays_for_detection_of_methamphetamine_methylenedioxyamphetamine_methylenedioxymethamphetamine_and_methylenedioxyethylamphetamine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/12.4.229 DB - PRIME DP - Unbound Medicine ER -