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Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays.
Food Chem. 2018 Mar 01; 242:378-388.FC

Abstract

The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r=0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r=0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9-2.0mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (-8.5kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50=0.10 and 2.37µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Mazewski C
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, USA. Electronic address: cmazews2@illlinois.edu.
Liang K
Department of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, USA. Electronic address: katiejliang@gmail.com.
Gonzalez de Mejia E
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, USA. Electronic address: edemejia@illinois.edu.

MeSH

AnthocyaninsCell ProliferationColonic NeoplasmsGlucosidesHCT116 CellsHT29 CellsHumansPhenolsPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29037704

Citation

Mazewski, Candice, et al. "Comparison of the Effect of Chemical Composition of Anthocyanin-rich Plant Extracts On Colon Cancer Cell Proliferation and Their Potential Mechanism of Action Using in Vitro, in Silico, and Biochemical Assays." Food Chemistry, vol. 242, 2018, pp. 378-388.
Mazewski C, Liang K, Gonzalez de Mejia E. Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays. Food Chem. 2018;242:378-388.
Mazewski, C., Liang, K., & Gonzalez de Mejia, E. (2018). Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays. Food Chemistry, 242, 378-388. https://doi.org/10.1016/j.foodchem.2017.09.086
Mazewski C, Liang K, Gonzalez de Mejia E. Comparison of the Effect of Chemical Composition of Anthocyanin-rich Plant Extracts On Colon Cancer Cell Proliferation and Their Potential Mechanism of Action Using in Vitro, in Silico, and Biochemical Assays. Food Chem. 2018 Mar 1;242:378-388. PubMed PMID: 29037704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays. AU - Mazewski,Candice, AU - Liang,Katie, AU - Gonzalez de Mejia,Elvira, Y1 - 2017/09/18/ PY - 2017/06/30/received PY - 2017/08/23/revised PY - 2017/09/16/accepted PY - 2017/10/18/entrez PY - 2017/10/19/pubmed PY - 2018/2/23/medline KW - Anthocyanins KW - Apoptosis KW - Black lentil KW - Colon cancer KW - Red grape KW - Sorghum KW - Tyrosine kinase SP - 378 EP - 388 JF - Food chemistry JO - Food Chem VL - 242 N2 - The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r=0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r=0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9-2.0mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (-8.5kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50=0.10 and 2.37µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/29037704/Comparison_of_the_effect_of_chemical_composition_of_anthocyanin_rich_plant_extracts_on_colon_cancer_cell_proliferation_and_their_potential_mechanism_of_action_using_in_vitro_in_silico_and_biochemical_assays_ DB - PRIME DP - Unbound Medicine ER -