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Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties.
Int J Biol Macromol. 2018 Feb; 107(Pt B):2044-2056.IJ

Abstract

The paper explained the microencapsulation of three different antigenic materials viz. Diphtheria toxoid (DT), whole cell pertussis antigens (PT and FHA) and tetanus toxoid (TT) by coacervation method using water soluble chitosan as a polymer crosslinked by vanillin/TPP co-crosslinkers for the development of oral trivalent DwPT vaccine. Instrumental characterization of chitosan microspheres suggested specific interaction with vanillin/TPP, higher thermal stability, amorphous nature, spherical morphology with size less than 2μm along with positive charge density offering mucoadhesive properties. Furthermore, PT and FHA showed higher encapsulation up to 94% followed by TT and DT. Cumulative release rate of DT was (68.47%), TT (73.67%), PT (43%) and FHA (53%). Release kinetics interpreted using DD solver program, indicated protein release followed first order kinetics and obeyed Korsmeyer-peppas model, stating fickian diffusion relates to diffusion, erosion and controlled release rate of the encapsulated toxoids. Application of formulations on caco-2 cell line showed negligible cytotoxic effect and efficient uptake of FITC labelled microspheres. The obtained in-vivo results suggests that the final trivalent DwPT formulation were having successful elicitation of both systemic (IgG) and mucosal (sIgA) immune response in balb/c mice. Overall studies indicated that DwPT formulation could be a suitable alternative to available injectable DaPT vaccine.

Authors+Show Affiliations

Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India.Department of Zoology, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India.Department of Zoology, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune-411028, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune-411028, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly Pune University), Pune-411 007, India. Electronic address: pdoshi@chem.unipune.ac.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29037872

Citation

Walke, Shilratna, et al. "Preparation and Characterization of Microencapsulated DwPT Trivalent Vaccine Using Water Soluble Chitosan and Its In-vitro and In-vivo Immunological Properties." International Journal of Biological Macromolecules, vol. 107, no. Pt B, 2018, pp. 2044-2056.
Walke S, Srivastava G, Routaray CB, et al. Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties. Int J Biol Macromol. 2018;107(Pt B):2044-2056.
Walke, S., Srivastava, G., Routaray, C. B., Dhavale, D., Pai, K., Doshi, J., Kumar, R., & Doshi, P. (2018). Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties. International Journal of Biological Macromolecules, 107(Pt B), 2044-2056. https://doi.org/10.1016/j.ijbiomac.2017.10.073
Walke S, et al. Preparation and Characterization of Microencapsulated DwPT Trivalent Vaccine Using Water Soluble Chitosan and Its In-vitro and In-vivo Immunological Properties. Int J Biol Macromol. 2018;107(Pt B):2044-2056. PubMed PMID: 29037872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties. AU - Walke,Shilratna, AU - Srivastava,Gopal, AU - Routaray,Chinmayee Bar, AU - Dhavale,Dilip, AU - Pai,Kalpana, AU - Doshi,Jignesh, AU - Kumar,Rakesh, AU - Doshi,Pooja, Y1 - 2017/10/13/ PY - 2017/06/20/received PY - 2017/09/28/revised PY - 2017/10/12/accepted PY - 2017/10/19/pubmed PY - 2018/7/28/medline PY - 2017/10/18/entrez KW - Trivalent vaccine KW - Water soluble chitosan KW - Whole cell pertussis SP - 2044 EP - 2056 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 107 IS - Pt B N2 - The paper explained the microencapsulation of three different antigenic materials viz. Diphtheria toxoid (DT), whole cell pertussis antigens (PT and FHA) and tetanus toxoid (TT) by coacervation method using water soluble chitosan as a polymer crosslinked by vanillin/TPP co-crosslinkers for the development of oral trivalent DwPT vaccine. Instrumental characterization of chitosan microspheres suggested specific interaction with vanillin/TPP, higher thermal stability, amorphous nature, spherical morphology with size less than 2μm along with positive charge density offering mucoadhesive properties. Furthermore, PT and FHA showed higher encapsulation up to 94% followed by TT and DT. Cumulative release rate of DT was (68.47%), TT (73.67%), PT (43%) and FHA (53%). Release kinetics interpreted using DD solver program, indicated protein release followed first order kinetics and obeyed Korsmeyer-peppas model, stating fickian diffusion relates to diffusion, erosion and controlled release rate of the encapsulated toxoids. Application of formulations on caco-2 cell line showed negligible cytotoxic effect and efficient uptake of FITC labelled microspheres. The obtained in-vivo results suggests that the final trivalent DwPT formulation were having successful elicitation of both systemic (IgG) and mucosal (sIgA) immune response in balb/c mice. Overall studies indicated that DwPT formulation could be a suitable alternative to available injectable DaPT vaccine. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29037872/Preparation_and_characterization_of_microencapsulated_DwPT_trivalent_vaccine_using_water_soluble_chitosan_and_its_in_vitro_and_in_vivo_immunological_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32239-0 DB - PRIME DP - Unbound Medicine ER -