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Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches.
J Appl Microbiol. 2018 Jan; 124(1):166-178.JA

Abstract

AIMS

This study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit Rhizopus stolonifer and the control of postharvest soft rot decay in peaches.

METHODS AND RESULTS

To investigate the antifungal mechanism, the effects of carvacrol and eugenol on the mycelium growth, leakages of cytoplasmic contents, mycelium morphology, cell membrane and membrane composition of R. stolonifer were studied. Carvacrol and eugenol both exhibited dose-dependent antifungal activity against R. stolonifer, carvacrol at a concentration of 2 μl per plant and eugenol at a concentration of 4 μl per plant inhibited fungal growth completely. The two essential oils (EOs) increased cell membrane penetrability and caused the leakage of cytoplasm, nucleic acid and protein content. The observation using scanning electron microscopy and fluorescent microscopy showed modification of the hyphal morphology and breakage of the cell plasma membrane. Decreased ergosterol contents confirmed that the two EOs could destroy the membrane of R. stolonifer. For the in vivo test, the inhibition of soft rot disease and the induction of defence-related enzymes were investigated. Carvacrol and eugenol significantly reduced the incidence and severity of soft rot decay in inoculated peaches. The best treatments for controlling soft rot decay were obtained at 0·5 μl l-1 for carvacrol and 1 μl l-1 for eugenol. The activities of defence-related enzymes in peaches were also enhanced by fumigation with two EOs.

CONCLUSION

This study showed that carvacrol and eugenol could effectively inhibit the growth of R. stolonifer in vitro and successfully control the incidence of soft rot decay in honey peaches.

SIGNIFICANCE AND IMPACT OF THE STUDY

The above findings may be the main antifungal mechanism of carvacrol and eugenol on R. stolonifer. Furthermore, carvacrol and eugenol are helpful for their commercial application on the preservation of fresh fruit.

Authors+Show Affiliations

College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29044849

Citation

Zhou, D, et al. "Carvacrol and Eugenol Effectively Inhibit Rhizopus Stolonifer and Control Postharvest Soft Rot Decay in Peaches." Journal of Applied Microbiology, vol. 124, no. 1, 2018, pp. 166-178.
Zhou D, Wang Z, Li M, et al. Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches. J Appl Microbiol. 2018;124(1):166-178.
Zhou, D., Wang, Z., Li, M., Xing, M., Xian, T., & Tu, K. (2018). Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches. Journal of Applied Microbiology, 124(1), 166-178. https://doi.org/10.1111/jam.13612
Zhou D, et al. Carvacrol and Eugenol Effectively Inhibit Rhizopus Stolonifer and Control Postharvest Soft Rot Decay in Peaches. J Appl Microbiol. 2018;124(1):166-178. PubMed PMID: 29044849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches. AU - Zhou,D, AU - Wang,Z, AU - Li,M, AU - Xing,M, AU - Xian,T, AU - Tu,K, Y1 - 2017/12/18/ PY - 2017/08/07/received PY - 2017/09/16/revised PY - 2017/10/09/accepted PY - 2017/10/19/pubmed PY - 2018/5/2/medline PY - 2017/10/19/entrez KW - Rhizopus stolonifer KW - carvacrol KW - eugenol KW - peach KW - soft rot decay SP - 166 EP - 178 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 124 IS - 1 N2 - AIMS: This study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit Rhizopus stolonifer and the control of postharvest soft rot decay in peaches. METHODS AND RESULTS: To investigate the antifungal mechanism, the effects of carvacrol and eugenol on the mycelium growth, leakages of cytoplasmic contents, mycelium morphology, cell membrane and membrane composition of R. stolonifer were studied. Carvacrol and eugenol both exhibited dose-dependent antifungal activity against R. stolonifer, carvacrol at a concentration of 2 μl per plant and eugenol at a concentration of 4 μl per plant inhibited fungal growth completely. The two essential oils (EOs) increased cell membrane penetrability and caused the leakage of cytoplasm, nucleic acid and protein content. The observation using scanning electron microscopy and fluorescent microscopy showed modification of the hyphal morphology and breakage of the cell plasma membrane. Decreased ergosterol contents confirmed that the two EOs could destroy the membrane of R. stolonifer. For the in vivo test, the inhibition of soft rot disease and the induction of defence-related enzymes were investigated. Carvacrol and eugenol significantly reduced the incidence and severity of soft rot decay in inoculated peaches. The best treatments for controlling soft rot decay were obtained at 0·5 μl l-1 for carvacrol and 1 μl l-1 for eugenol. The activities of defence-related enzymes in peaches were also enhanced by fumigation with two EOs. CONCLUSION: This study showed that carvacrol and eugenol could effectively inhibit the growth of R. stolonifer in vitro and successfully control the incidence of soft rot decay in honey peaches. SIGNIFICANCE AND IMPACT OF THE STUDY: The above findings may be the main antifungal mechanism of carvacrol and eugenol on R. stolonifer. Furthermore, carvacrol and eugenol are helpful for their commercial application on the preservation of fresh fruit. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/29044849/Carvacrol_and_eugenol_effectively_inhibit_Rhizopus_stolonifer_and_control_postharvest_soft_rot_decay_in_peaches_ L2 - https://doi.org/10.1111/jam.13612 DB - PRIME DP - Unbound Medicine ER -