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Exercise induces TFEB expression and activity in skeletal muscle in a PGC-1α-dependent manner.
Am J Physiol Cell Physiol 2018; 314(1):C62-C72AJ

Abstract

The mitochondrial network in muscle is controlled by the opposing processes of mitochondrial biogenesis and mitophagy. The coactivator peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) regulates biogenesis, while the transcription of mitophagy-related genes is controlled by transcription factor EB (TFEB). PGC-1α activation is induced by exercise; however, the effect of exercise on TFEB is not fully known. We investigated the interplay between PGC-1α and TFEB on mitochondria in response to acute contractile activity in C2C12 myotubes and following exercise in wild-type and PGC-1α knockout mice. TFEB nuclear localization was increased by 1.6-fold following 2 h of acute myotube contractile activity in culture, while TFEB transcription was also simultaneously increased by twofold to threefold. Viral overexpression of TFEB in myotubes increased PGC-1α and cytochrome- c oxidase-IV gene expression. In wild-type mice, TFEB translocation to the nucleus increased 2.4-fold in response to acute exercise, while TFEB transcription, assessed through the electroporation of a TFEB promoter construct, was elevated by fourfold. These exercise effects were dependent on the presence of PGC-1α. Our data indicate that acute exercise provokes TFEB expression and activation in a PGC-1α-dependent manner and suggest that TFEB, along with PGC-1α, is an important regulator of mitochondrial biogenesis in muscle as a result of exercise.

Authors+Show Affiliations

Muscle Health Research Centre, School of Kinesiology and Health Science, York University , Toronto, Ontario , Canada.Muscle Health Research Centre, School of Kinesiology and Health Science, York University , Toronto, Ontario , Canada.Muscle Health Research Centre, School of Kinesiology and Health Science, York University , Toronto, Ontario , Canada.Muscle Health Research Centre, School of Kinesiology and Health Science, York University , Toronto, Ontario , Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29046293

Citation

Erlich, Avigail T., et al. "Exercise Induces TFEB Expression and Activity in Skeletal Muscle in a PGC-1α-dependent Manner." American Journal of Physiology. Cell Physiology, vol. 314, no. 1, 2018, pp. C62-C72.
Erlich AT, Brownlee DM, Beyfuss K, et al. Exercise induces TFEB expression and activity in skeletal muscle in a PGC-1α-dependent manner. Am J Physiol, Cell Physiol. 2018;314(1):C62-C72.
Erlich, A. T., Brownlee, D. M., Beyfuss, K., & Hood, D. A. (2018). Exercise induces TFEB expression and activity in skeletal muscle in a PGC-1α-dependent manner. American Journal of Physiology. Cell Physiology, 314(1), pp. C62-C72. doi:10.1152/ajpcell.00162.2017.
Erlich AT, et al. Exercise Induces TFEB Expression and Activity in Skeletal Muscle in a PGC-1α-dependent Manner. Am J Physiol, Cell Physiol. 2018 01 1;314(1):C62-C72. PubMed PMID: 29046293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exercise induces TFEB expression and activity in skeletal muscle in a PGC-1α-dependent manner. AU - Erlich,Avigail T, AU - Brownlee,Diane M, AU - Beyfuss,Kaitlyn, AU - Hood,David A, Y1 - 2017/10/18/ PY - 2017/10/20/pubmed PY - 2019/2/27/medline PY - 2017/10/20/entrez KW - autophagy KW - contractile activity, transcription KW - mitochondrial biogenesis KW - mitophagy KW - peroxisome proliferator-activated receptor-γ coactivator-1α KW - transcription factor EB SP - C62 EP - C72 JF - American journal of physiology. Cell physiology JO - Am. J. Physiol., Cell Physiol. VL - 314 IS - 1 N2 - The mitochondrial network in muscle is controlled by the opposing processes of mitochondrial biogenesis and mitophagy. The coactivator peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) regulates biogenesis, while the transcription of mitophagy-related genes is controlled by transcription factor EB (TFEB). PGC-1α activation is induced by exercise; however, the effect of exercise on TFEB is not fully known. We investigated the interplay between PGC-1α and TFEB on mitochondria in response to acute contractile activity in C2C12 myotubes and following exercise in wild-type and PGC-1α knockout mice. TFEB nuclear localization was increased by 1.6-fold following 2 h of acute myotube contractile activity in culture, while TFEB transcription was also simultaneously increased by twofold to threefold. Viral overexpression of TFEB in myotubes increased PGC-1α and cytochrome- c oxidase-IV gene expression. In wild-type mice, TFEB translocation to the nucleus increased 2.4-fold in response to acute exercise, while TFEB transcription, assessed through the electroporation of a TFEB promoter construct, was elevated by fourfold. These exercise effects were dependent on the presence of PGC-1α. Our data indicate that acute exercise provokes TFEB expression and activation in a PGC-1α-dependent manner and suggest that TFEB, along with PGC-1α, is an important regulator of mitochondrial biogenesis in muscle as a result of exercise. SN - 1522-1563 UR - https://www.unboundmedicine.com/medline/citation/29046293/Exercise_induces_TFEB_expression_and_activity_in_skeletal_muscle_in_a_PGC_1α_dependent_manner_ L2 - http://www.physiology.org/doi/full/10.1152/ajpcell.00162.2017?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -