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PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle.
PLoS One 2017; 12(10):e0185993Plos

Abstract

The aim of the present study was to examine the role of PGC-1α in intensity dependent exercise and exercise training-induced metabolic adaptations in mouse skeletal muscle. Whole body PGC-1α knockout (KO) and littermate wildtype (WT) mice performed a single treadmill running bout at either low intensity (LI) for 40 min or moderate intensity (MI) for 20 min. Blood and quadriceps muscles were removed either immediately after exercise or at 3h or 6h into recovery from exercise and from resting controls. In addition PGC-1α KO and littermate WT mice were exercise trained at either low intensity (LIT) for 40 min or at moderate intensity (MIT) for 20 min 2 times pr. day for 5 weeks. In the first and the last week of the intervention period, mice performed a graded running endurance test. Quadriceps muscles were removed before and after the training period for analyses. The acute exercise bout elicited intensity dependent increases in LC3I and LC3II protein and intensity independent decrease in p62 protein in skeletal muscle late in recovery and increased LC3II with exercise training independent of exercise intensity and volume in WT mice. Furthermore, acute exercise and exercise training did not increase LC3I and LC3II protein in PGC-1α KO. In addition, exercise-induced mRNA responses of PGC-1α isoforms were intensity dependent. In conclusion, these findings indicate that exercise intensity affected autophagy markers differently in skeletal muscle and suggest that PGC-1α regulates both acute and exercise training-induced autophagy in skeletal muscle potentially in a PGC-1α isoform specific manner.

Authors+Show Affiliations

The August Krogh Club, Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Universitetsparken 13, Copenhagen Ø, Denmark.The August Krogh Club, Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Universitetsparken 13, Copenhagen Ø, Denmark.Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Universitetsparken 13, Copenhagen Ø, Denmark.The August Krogh Club, Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Universitetsparken 13, Copenhagen Ø, Denmark.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29049322

Citation

Brandt, Nina, et al. "PGC-1α and Exercise Intensity Dependent Adaptations in Mouse Skeletal Muscle." PloS One, vol. 12, no. 10, 2017, pp. e0185993.
Brandt N, Dethlefsen MM, Bangsbo J, et al. PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle. PLoS ONE. 2017;12(10):e0185993.
Brandt, N., Dethlefsen, M. M., Bangsbo, J., & Pilegaard, H. (2017). PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle. PloS One, 12(10), pp. e0185993. doi:10.1371/journal.pone.0185993.
Brandt N, et al. PGC-1α and Exercise Intensity Dependent Adaptations in Mouse Skeletal Muscle. PLoS ONE. 2017;12(10):e0185993. PubMed PMID: 29049322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle. AU - Brandt,Nina, AU - Dethlefsen,Maja Munk, AU - Bangsbo,Jens, AU - Pilegaard,Henriette, Y1 - 2017/10/19/ PY - 2017/02/27/received PY - 2017/09/22/accepted PY - 2017/10/20/entrez PY - 2017/10/20/pubmed PY - 2017/11/8/medline SP - e0185993 EP - e0185993 JF - PloS one JO - PLoS ONE VL - 12 IS - 10 N2 - The aim of the present study was to examine the role of PGC-1α in intensity dependent exercise and exercise training-induced metabolic adaptations in mouse skeletal muscle. Whole body PGC-1α knockout (KO) and littermate wildtype (WT) mice performed a single treadmill running bout at either low intensity (LI) for 40 min or moderate intensity (MI) for 20 min. Blood and quadriceps muscles were removed either immediately after exercise or at 3h or 6h into recovery from exercise and from resting controls. In addition PGC-1α KO and littermate WT mice were exercise trained at either low intensity (LIT) for 40 min or at moderate intensity (MIT) for 20 min 2 times pr. day for 5 weeks. In the first and the last week of the intervention period, mice performed a graded running endurance test. Quadriceps muscles were removed before and after the training period for analyses. The acute exercise bout elicited intensity dependent increases in LC3I and LC3II protein and intensity independent decrease in p62 protein in skeletal muscle late in recovery and increased LC3II with exercise training independent of exercise intensity and volume in WT mice. Furthermore, acute exercise and exercise training did not increase LC3I and LC3II protein in PGC-1α KO. In addition, exercise-induced mRNA responses of PGC-1α isoforms were intensity dependent. In conclusion, these findings indicate that exercise intensity affected autophagy markers differently in skeletal muscle and suggest that PGC-1α regulates both acute and exercise training-induced autophagy in skeletal muscle potentially in a PGC-1α isoform specific manner. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29049322/PGC_1α_and_exercise_intensity_dependent_adaptations_in_mouse_skeletal_muscle_ L2 - http://dx.plos.org/10.1371/journal.pone.0185993 DB - PRIME DP - Unbound Medicine ER -