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Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus * Phoma macdonaldii} interaction case for black stem disease and premature ripening.
BMC Plant Biol. 2017 Oct 19; 17(1):167.BP

Abstract

BACKGROUND

Phoma macdonaldii has been reported as the causal agent of black stem disease (BS) and premature ripening (PR) on sunflower. PR is considered as the most widespread and detrimental disease on sunflower in France. While genetic variability and QTL mapping for partial resistance of sunflower to stem, collar and roots attacks have been reported on plantlets in controlled conditions, this work aims to describe the genetic variability in a subset of a sunflower lines, and for the first time to map QTL involved in PR resistance evaluated in field conditions using controlled inoculation.

RESULTS

An efficient and reliable method for inoculation used in field experiments induced stem base necrosis on up to 98% of all plants. A significant genetic variability for PR resistance in the field was detected among the 20 inbred lines of the core collection tested across the two years. For QTL mapping, the PR resistance evaluation was performed on two recombinant inbred lines (RIL) populations derived from the crosses XRQxPSC8 and FUxPAZ2 in two different years. QTL analyses were based on a newly developed consensus genetic map comprising 1007 non-redundant molecular markers. In each of the two RIL populations, different QTL involved in PR partial sunflower resistance were detected. The most significant QTL were detected 49 days post infection (DPI) on LG10 (LOD 7.7) and on LG7 (LOD 12.1) in the XRQxPSC8 and FUxPAZ2 RIL population, respectively. In addition, different QTL were detected on both populations for PR resistance measured between 14 and 35 DPI. In parallel, the incidence of natural attack of P. macdonaldii resulting in BS disease was recorded, showing that in these populations, the genetic of resistance to both diseases is not governed by the same factors.

CONCLUSION

This work provides the first insights on the genetic architecture of sunflower PR resistance in the field. Moreover, the separate studies of symptoms on different organs and in time series allowed the identification of a succession of genetic components involved in the sunflower resistance to PR and BS diseases caused by Phoma macdonaldii along the development of the {plant * pathogen} interaction.

Authors+Show Affiliations

LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France. Present address: INRA, Université de Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d'Ornon, France.LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France. Present address: EURALIS Semences, Domaine de Sandreau, 6 Chemin de Panedautes, 31700, Mondonville, France.LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.ENSAT, UMR 1248 AGIR, BP52627, F-31326, Castanet-Tolosan, France. AGIR, Université de Toulouse, INRA, INPT, INP-EI PURPAN, Castanet-Tolosan, France.LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.Terres Inovia, UMR 1248 AGIR, BP52627, F-31326, Castanet-Tolosan, France. e.mestries@terresinovia.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29052528

Citation

Bordat, Amandine, et al. "Different Genetic Architectures Underlie Crop Responses to the Same Pathogen: the {Helianthus Annuus * Phoma Macdonaldii} Interaction Case for Black Stem Disease and Premature Ripening." BMC Plant Biology, vol. 17, no. 1, 2017, p. 167.
Bordat A, Marchand G, Langlade NB, et al. Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus * Phoma macdonaldii} interaction case for black stem disease and premature ripening. BMC Plant Biol. 2017;17(1):167.
Bordat, A., Marchand, G., Langlade, N. B., Pouilly, N., Muños, S., Dechamp-Guillaume, G., Vincourt, P., & Bret-Mestries, E. (2017). Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus * Phoma macdonaldii} interaction case for black stem disease and premature ripening. BMC Plant Biology, 17(1), 167. https://doi.org/10.1186/s12870-017-1116-1
Bordat A, et al. Different Genetic Architectures Underlie Crop Responses to the Same Pathogen: the {Helianthus Annuus * Phoma Macdonaldii} Interaction Case for Black Stem Disease and Premature Ripening. BMC Plant Biol. 2017 Oct 19;17(1):167. PubMed PMID: 29052528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus * Phoma macdonaldii} interaction case for black stem disease and premature ripening. AU - Bordat,Amandine, AU - Marchand,Gwenaëlle, AU - Langlade,Nicolas B, AU - Pouilly,Nicolas, AU - Muños,Stéphane, AU - Dechamp-Guillaume,Grégory, AU - Vincourt,Patrick, AU - Bret-Mestries,Emmanuelle, Y1 - 2017/10/19/ PY - 2017/04/07/received PY - 2017/10/09/accepted PY - 2017/10/21/entrez PY - 2017/10/21/pubmed PY - 2018/6/6/medline KW - Black stem KW - Phoma macdonaldii KW - Premature ripening KW - QTL mapping KW - Sunflower SP - 167 EP - 167 JF - BMC plant biology JO - BMC Plant Biol. VL - 17 IS - 1 N2 - BACKGROUND: Phoma macdonaldii has been reported as the causal agent of black stem disease (BS) and premature ripening (PR) on sunflower. PR is considered as the most widespread and detrimental disease on sunflower in France. While genetic variability and QTL mapping for partial resistance of sunflower to stem, collar and roots attacks have been reported on plantlets in controlled conditions, this work aims to describe the genetic variability in a subset of a sunflower lines, and for the first time to map QTL involved in PR resistance evaluated in field conditions using controlled inoculation. RESULTS: An efficient and reliable method for inoculation used in field experiments induced stem base necrosis on up to 98% of all plants. A significant genetic variability for PR resistance in the field was detected among the 20 inbred lines of the core collection tested across the two years. For QTL mapping, the PR resistance evaluation was performed on two recombinant inbred lines (RIL) populations derived from the crosses XRQxPSC8 and FUxPAZ2 in two different years. QTL analyses were based on a newly developed consensus genetic map comprising 1007 non-redundant molecular markers. In each of the two RIL populations, different QTL involved in PR partial sunflower resistance were detected. The most significant QTL were detected 49 days post infection (DPI) on LG10 (LOD 7.7) and on LG7 (LOD 12.1) in the XRQxPSC8 and FUxPAZ2 RIL population, respectively. In addition, different QTL were detected on both populations for PR resistance measured between 14 and 35 DPI. In parallel, the incidence of natural attack of P. macdonaldii resulting in BS disease was recorded, showing that in these populations, the genetic of resistance to both diseases is not governed by the same factors. CONCLUSION: This work provides the first insights on the genetic architecture of sunflower PR resistance in the field. Moreover, the separate studies of symptoms on different organs and in time series allowed the identification of a succession of genetic components involved in the sunflower resistance to PR and BS diseases caused by Phoma macdonaldii along the development of the {plant * pathogen} interaction. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/29052528/Different_genetic_architectures_underlie_crop_responses_to_the_same_pathogen:_the_{Helianthus_annuus___Phoma_macdonaldii}_interaction_case_for_black_stem_disease_and_premature_ripening_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-017-1116-1 DB - PRIME DP - Unbound Medicine ER -