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Protective Effect of Flavonoids from Ziziphus jujuba cv. Jinsixiaozao against Acetaminophen-Induced Liver Injury by Inhibiting Oxidative Stress and Inflammation in Mice.
Molecules. 2017 Oct 20; 22(10)M

Abstract

This study was aimed to investigate the chemical composition, antioxidant activities and hepatoprotective effect of flavonoids from Ziziphus jujuba cv. Jinsixiaozao (ZJF). The composition of ZJF was analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography-mass spectrometry (LC-MS), and antioxidant properties were investigated by biological assays in vitro. The hepatoprotective activity of ZJF was evaluated in acetaminophen (APAP)-treated BALB/c mice. Results indicate that ZJF displayed significant antioxidant capacity. Pretreatment with ZJF significantly decreased APAP-elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (TB). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were enhanced with ZJF administration, while malondialdehyde (MDA) level and glutathione (GSH) depletion were reduced. Meanwhile, ZJF reversed the suppression of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation, and up-regulated the protein expression of NAD(P)H: quinone oxidoreductase 1(NQO1) in liver damage mice. Furthermore, ZJF attenuated APAP-induced inflammatory mediator production, such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Expression of p65 showed that ZJF dampened nuclear factor-κB (NF-κB) activation. The results strongly indicate that the hepatoprotective role of ZJF in APAP-induced hepatotoxicity might result from its induction of antioxidant defense via activation of Nrf2 and reduction of inflammation via inhibition of NF-κB.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 50012, Shandong, China. jiangxiaoyan1121@163.com.School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 50012, Shandong, China. 13021717075@163.com.School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 50012, Shandong, China. jiangxiaoyan1121@163.com.School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 50012, Shandong, China. 13021717075@163.com.School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 50012, Shandong, China. zxzhao@sdu.edu.cn. Shandong Engineering & Technology Research Center for Jujube Food and Drug, 44 West Wenhua Road, Jinan 250012, Shandong, China. zxzhao@sdu.edu.cn. Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technologies, Shandong Academy of Pharmaceutical Sciences, 989 Xinluo Street, Jinan 250101, Shandong, China. zxzhao@sdu.edu.cn.Department of Pathology and Pathophysiology, School of Basic Medicine, Shandong University, 44 West Wenhua Road, Jinan 250012, Shandong, China. oruszxzhao@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29053632

Citation

Huang, Weizhen, et al. "Protective Effect of Flavonoids From Ziziphus Jujuba Cv. Jinsixiaozao Against Acetaminophen-Induced Liver Injury By Inhibiting Oxidative Stress and Inflammation in Mice." Molecules (Basel, Switzerland), vol. 22, no. 10, 2017.
Huang W, Wang Y, Jiang X, et al. Protective Effect of Flavonoids from Ziziphus jujuba cv. Jinsixiaozao against Acetaminophen-Induced Liver Injury by Inhibiting Oxidative Stress and Inflammation in Mice. Molecules. 2017;22(10).
Huang, W., Wang, Y., Jiang, X., Sun, Y., Zhao, Z., & Li, S. (2017). Protective Effect of Flavonoids from Ziziphus jujuba cv. Jinsixiaozao against Acetaminophen-Induced Liver Injury by Inhibiting Oxidative Stress and Inflammation in Mice. Molecules (Basel, Switzerland), 22(10). https://doi.org/10.3390/molecules22101781
Huang W, et al. Protective Effect of Flavonoids From Ziziphus Jujuba Cv. Jinsixiaozao Against Acetaminophen-Induced Liver Injury By Inhibiting Oxidative Stress and Inflammation in Mice. Molecules. 2017 Oct 20;22(10) PubMed PMID: 29053632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Flavonoids from Ziziphus jujuba cv. Jinsixiaozao against Acetaminophen-Induced Liver Injury by Inhibiting Oxidative Stress and Inflammation in Mice. AU - Huang,Weizhen, AU - Wang,Yongjie, AU - Jiang,Xiaoyan, AU - Sun,Yueyue, AU - Zhao,Zhongxi, AU - Li,Siying, Y1 - 2017/10/20/ PY - 2017/10/03/received PY - 2017/10/17/revised PY - 2017/10/17/accepted PY - 2017/10/21/entrez PY - 2017/10/21/pubmed PY - 2018/6/7/medline KW - Flavonoids KW - NF-κB KW - Nrf2 KW - Ziziphus jujuba cv. Jinsixiaozao KW - anti-inflammation KW - antioxidant activity KW - hepatoprotective JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 10 N2 - This study was aimed to investigate the chemical composition, antioxidant activities and hepatoprotective effect of flavonoids from Ziziphus jujuba cv. Jinsixiaozao (ZJF). The composition of ZJF was analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography-mass spectrometry (LC-MS), and antioxidant properties were investigated by biological assays in vitro. The hepatoprotective activity of ZJF was evaluated in acetaminophen (APAP)-treated BALB/c mice. Results indicate that ZJF displayed significant antioxidant capacity. Pretreatment with ZJF significantly decreased APAP-elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (TB). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were enhanced with ZJF administration, while malondialdehyde (MDA) level and glutathione (GSH) depletion were reduced. Meanwhile, ZJF reversed the suppression of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation, and up-regulated the protein expression of NAD(P)H: quinone oxidoreductase 1(NQO1) in liver damage mice. Furthermore, ZJF attenuated APAP-induced inflammatory mediator production, such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Expression of p65 showed that ZJF dampened nuclear factor-κB (NF-κB) activation. The results strongly indicate that the hepatoprotective role of ZJF in APAP-induced hepatotoxicity might result from its induction of antioxidant defense via activation of Nrf2 and reduction of inflammation via inhibition of NF-κB. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/29053632/Protective_Effect_of_Flavonoids_from_Ziziphus_jujuba_cv__Jinsixiaozao_against_Acetaminophen_Induced_Liver_Injury_by_Inhibiting_Oxidative_Stress_and_Inflammation_in_Mice_ L2 - https://www.mdpi.com/resolver?pii=molecules22101781 DB - PRIME DP - Unbound Medicine ER -