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Diagnostic relevance of IgE sensitization profiles to eight recombinant Phleum pratense molecules.
Allergy. 2018 03; 73(3):673-682.A

Abstract

BACKGROUND

Grass pollen-related seasonal allergic rhinoconjunctivitis (SARg) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component-resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed at analyzing the characteristics of the IgE sensitization to Phleum pratense molecules and investigating the diagnostic relevance of such molecules in childhood.

METHODS

We examined 1120 children (age 4-18 years) with SARg. Standardized questionnaires on atopy were acquired through informatics platform (AllergyCARD™). Skin prick tests were performed with pollen extracts. Serum IgE to airborne allergens and eight P. pratense molecules (rPhl p 1, rPhl p 2, rPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12) were tested by ImmunoCAP FEIA.

RESULTS

The analysis of IgE responses against eight P. pratense molecules showed 87 profiles. According to the number of molecules recognized by IgE, the more complex profiles were characterized by higher serum total IgE, higher grass-specific serum IgE, and higher number and degree of sensitization to pollens. The most frequent IgE sensitization profile was the monomolecular Phl p 1. Sensitization to Phl p 7 was a reliable biomarker of asthma, whereas Phl p 12 of oral allergy syndrome. Sensitization to Phl p 7 was associated with a higher severity of SARg, and complex profiles were associated with longer disease duration.

CONCLUSIONS

In a large pediatric population, the complexity of IgE sensitization profiles against P. pratense molecules is related to high atopic features although useless for predicting the clinical severity. The detection of serum IgE to Phl p 1, Phl p 7, and Phl p 12 can be used as clinical biomarkers of SARg and comorbidities. Further studies in different areas are required to test the impact of different IgE molecular profiles on AIT response.

Authors+Show Affiliations

Department of Pediatric Pneumology and Immunology, Charité Medical University, Berlin, Germany. Pediatric Unit, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.Department of Pediatric Pneumology and Immunology, Charité Medical University, Berlin, Germany. Pediatric Clinic of Medicine and Surgery, University of Parma, Parma, Italy.Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.Pediatric Unit, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.Department of Pediatric Pneumology and Immunology, Charité Medical University, Berlin, Germany.Biostatistics, L'Altra Statistica srl, Consultancy & Training, Rome, Italy.Allergology Service, San Carlo Clinic, Paderno Dugnano, Milan, Italy.Pediatric Emergency Unit, S. Orsola-Malpighi Hospital, Bologna, Italy.Pediatric Unit, San Camillo Forlanini Hospital, Rome, Italy.Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Università della Campania Luigi Vanvitelli, Naples, Italy.Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Università della Campania Luigi Vanvitelli, Naples, Italy.Pediatric Department, La Sapienza University, Rome, Italy.Pediatric Department, La Sapienza University, Rome, Italy.Pediatric Department, La Sapienza University, Rome, Italy.Pediatric Unit, Fatebenefratelli Hospital, Benevento, Italy.Pediatric Highly Intensive Care Unit, Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.Pediatric Intermediate Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.Pediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.Pediatric Allergology and Immunology Unit, Policlinico Tor Vergata, University of Rome Tor Vergata, Rome, Italy.Pediatric Allergology and Immunology Unit, Policlinico Tor Vergata, University of Rome Tor Vergata, Rome, Italy.Pediatric Unit, San Giuseppe Hospital, Empoli, Italy.Pediatric Unit, Grassi Hospital, Rome, Italy.Pediatric Unit, Santa Barbara Hospital, Iglesias, Italy.Pulmonary Disease and Allergy Unit, G. Gaslini Hospital, Genoa, Italy.Pediatric Unit, Crotone, Italy.Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.Pediatric Department and Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.Pediatric Clinic of Medicine and Surgery, University of Parma, Parma, Italy.Pediatric Section, Department of Life and Reproduction Sciences, University of Verona, Verona, Italy.Pediatric Department, La Sapienza University, Rome, Italy.Pediatric Department, La Sapienza University, Rome, Italy.Primary Care Pediatrics, ASL Salerno, Vietri sul Mare, Italy.Department of Laboratory Medicine, University Hospital of Padua, Padua, Italy.Department of Laboratory Medicine, University Hospital of Padua, Padua, Italy.Pediatric Unit, San Camillo Forlanini Hospital, Rome, Italy.Pediatric Clinic of Medicine and Surgery, University of Parma, Parma, Italy.Department of Pediatric Pneumology and Immunology, Charité Medical University, Berlin, Germany.No affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Observational Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29055045

Citation

Cipriani, F, et al. "Diagnostic Relevance of IgE Sensitization Profiles to Eight Recombinant Phleum Pratense Molecules." Allergy, vol. 73, no. 3, 2018, pp. 673-682.
Cipriani F, Mastrorilli C, Tripodi S, et al. Diagnostic relevance of IgE sensitization profiles to eight recombinant Phleum pratense molecules. Allergy. 2018;73(3):673-682.
Cipriani, F., Mastrorilli, C., Tripodi, S., Ricci, G., Perna, S., Panetta, V., Asero, R., Dondi, A., Bianchi, A., Maiello, N., Miraglia Del Giudice, M., Frediani, T., Macrì, F., Lucarelli, S., Dello Iacono, I., Patria, M. F., Varin, E., Peroni, D., Chini, L., ... Matricardi, P. M. (2018). Diagnostic relevance of IgE sensitization profiles to eight recombinant Phleum pratense molecules. Allergy, 73(3), 673-682. https://doi.org/10.1111/all.13338
Cipriani F, et al. Diagnostic Relevance of IgE Sensitization Profiles to Eight Recombinant Phleum Pratense Molecules. Allergy. 2018;73(3):673-682. PubMed PMID: 29055045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic relevance of IgE sensitization profiles to eight recombinant Phleum pratense molecules. AU - Cipriani,F, AU - Mastrorilli,C, AU - Tripodi,S, AU - Ricci,G, AU - Perna,S, AU - Panetta,V, AU - Asero,R, AU - Dondi,A, AU - Bianchi,A, AU - Maiello,N, AU - Miraglia Del Giudice,M, AU - Frediani,T, AU - Macrì,F, AU - Lucarelli,S, AU - Dello Iacono,I, AU - Patria,M F, AU - Varin,E, AU - Peroni,D, AU - Chini,L, AU - Moschese,V, AU - Bernardini,R, AU - Pingitore,G, AU - Pelosi,U, AU - Tosca,M, AU - Paravati,F, AU - Sfika,I, AU - Businco,A Di Rienzo, AU - Povesi Dascola,C, AU - Comberiati,P, AU - Frediani,S, AU - Lambiase,C, AU - Verga,M C, AU - Faggian,D, AU - Plebani,M, AU - Calvani,M, AU - Caffarelli,C, AU - Matricardi,P M, AU - ,, Y1 - 2017/12/14/ PY - 2017/10/16/accepted PY - 2017/10/22/pubmed PY - 2019/5/11/medline PY - 2017/10/22/entrez KW - IgE sensitization profiles KW - allergy KW - children KW - component-resolved diagnostics KW - grass pollen SP - 673 EP - 682 JF - Allergy JO - Allergy VL - 73 IS - 3 N2 - BACKGROUND: Grass pollen-related seasonal allergic rhinoconjunctivitis (SARg) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component-resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed at analyzing the characteristics of the IgE sensitization to Phleum pratense molecules and investigating the diagnostic relevance of such molecules in childhood. METHODS: We examined 1120 children (age 4-18 years) with SARg. Standardized questionnaires on atopy were acquired through informatics platform (AllergyCARD™). Skin prick tests were performed with pollen extracts. Serum IgE to airborne allergens and eight P. pratense molecules (rPhl p 1, rPhl p 2, rPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12) were tested by ImmunoCAP FEIA. RESULTS: The analysis of IgE responses against eight P. pratense molecules showed 87 profiles. According to the number of molecules recognized by IgE, the more complex profiles were characterized by higher serum total IgE, higher grass-specific serum IgE, and higher number and degree of sensitization to pollens. The most frequent IgE sensitization profile was the monomolecular Phl p 1. Sensitization to Phl p 7 was a reliable biomarker of asthma, whereas Phl p 12 of oral allergy syndrome. Sensitization to Phl p 7 was associated with a higher severity of SARg, and complex profiles were associated with longer disease duration. CONCLUSIONS: In a large pediatric population, the complexity of IgE sensitization profiles against P. pratense molecules is related to high atopic features although useless for predicting the clinical severity. The detection of serum IgE to Phl p 1, Phl p 7, and Phl p 12 can be used as clinical biomarkers of SARg and comorbidities. Further studies in different areas are required to test the impact of different IgE molecular profiles on AIT response. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/29055045/Diagnostic_relevance_of_IgE_sensitization_profiles_to_eight_recombinant_Phleum_pratense_molecules_ L2 - https://doi.org/10.1111/all.13338 DB - PRIME DP - Unbound Medicine ER -