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Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease.
Biomed Res Int. 2017; 2017:8030369.BR

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs, which regulate numerous cell functions by targeting mRNA for cleavage or translational repression, and have been found to play an important role in Alzheimer's disease (AD). Our study aimed to identify differentially expressed miRNAs in AD brain as a reference of potential therapeutic miRNAs or biomarkers for this disease. We used amyloid precursor protein (APP) and presenilin 1 (PS1) double transgenic mice and age-matched wild-type (WT) littermates to determine the expression of miRNAs in the brain. MiRNAs were profiled by microarray, and differentially expressed miRNAs underwent target prediction and enrichment analysis. Microarray analysis revealed 56 differentially expressed miRNAs in AD mouse brain, which involved 39 miRNAs that were significantly upregulated and 19 that were downregulated at different ages. Among those miRNAs, a total of 11 miRNAs, including miR-342-3p, miR-342-5p, miR-376c-3p, and miR-301b-3p, were not only conserved in human but also predicted to have targets and signaling pathways closely related to the pathology of AD. In conclusion, in this study, differentially expressed miRNAs were identified in AD brain and proposed as biomarkers, which may have the potential to indicate AD progression. Despite being preliminary, these results may aid in investigating pathological hallmarks and identify effective therapeutic targets.

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Authors+Show Affiliations

Wang LL
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Min L
Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China.
Guo QD
Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China.
Zhang JX
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Jiang HL
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Shao S
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Xing JG
Key Laboratory of Uighur Medicine of Xinjiang Uygur Autonomous Region, Xinjiang Institute of Materia Medica, Urumqi 830004, China.
Yin LL
Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Liu JH
Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
Liu R
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Guo SL
Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, China.

MeSH

Alzheimer DiseaseAnimalsBiomarkersBrainDisease Models, AnimalGene Expression ProfilingGene Expression RegulationHumansMiceMice, TransgenicMicroRNAsTissue Array Analysis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29057267

Citation

Wang, Lin-Lin, et al. "Profiling microRNA From Brain By Microarray in a Transgenic Mouse Model of Alzheimer's Disease." BioMed Research International, vol. 2017, 2017, p. 8030369.
Wang LL, Min L, Guo QD, et al. Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease. Biomed Res Int. 2017;2017:8030369.
Wang, L. L., Min, L., Guo, Q. D., Zhang, J. X., Jiang, H. L., Shao, S., Xing, J. G., Yin, L. L., Liu, J. H., Liu, R., & Guo, S. L. (2017). Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease. BioMed Research International, 2017, 8030369. https://doi.org/10.1155/2017/8030369
Wang LL, et al. Profiling microRNA From Brain By Microarray in a Transgenic Mouse Model of Alzheimer's Disease. Biomed Res Int. 2017;2017:8030369. PubMed PMID: 29057267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling microRNA from Brain by Microarray in a Transgenic Mouse Model of Alzheimer's Disease. AU - Wang,Lin-Lin, AU - Min,Li, AU - Guo,Qing-Dong, AU - Zhang,Jun-Xia, AU - Jiang,Hai-Lun, AU - Shao,Shuai, AU - Xing,Jian-Guo, AU - Yin,Lin-Lin, AU - Liu,Jiang-Hong, AU - Liu,Rui, AU - Guo,Shui-Long, Y1 - 2017/09/19/ PY - 2017/05/31/received PY - 2017/08/09/accepted PY - 2017/10/24/entrez PY - 2017/10/24/pubmed PY - 2018/6/8/medline SP - 8030369 EP - 8030369 JF - BioMed research international JO - Biomed Res Int VL - 2017 N2 - MicroRNAs (miRNAs) are small noncoding RNAs, which regulate numerous cell functions by targeting mRNA for cleavage or translational repression, and have been found to play an important role in Alzheimer's disease (AD). Our study aimed to identify differentially expressed miRNAs in AD brain as a reference of potential therapeutic miRNAs or biomarkers for this disease. We used amyloid precursor protein (APP) and presenilin 1 (PS1) double transgenic mice and age-matched wild-type (WT) littermates to determine the expression of miRNAs in the brain. MiRNAs were profiled by microarray, and differentially expressed miRNAs underwent target prediction and enrichment analysis. Microarray analysis revealed 56 differentially expressed miRNAs in AD mouse brain, which involved 39 miRNAs that were significantly upregulated and 19 that were downregulated at different ages. Among those miRNAs, a total of 11 miRNAs, including miR-342-3p, miR-342-5p, miR-376c-3p, and miR-301b-3p, were not only conserved in human but also predicted to have targets and signaling pathways closely related to the pathology of AD. In conclusion, in this study, differentially expressed miRNAs were identified in AD brain and proposed as biomarkers, which may have the potential to indicate AD progression. Despite being preliminary, these results may aid in investigating pathological hallmarks and identify effective therapeutic targets. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/29057267/Profiling_microRNA_from_Brain_by_Microarray_in_a_Transgenic_Mouse_Model_of_Alzheimer's_Disease_ DB - PRIME DP - Unbound Medicine ER -