Tags

Type your tag names separated by a space and hit enter

secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus.
Microbiologyopen 2018; 7(2):e00551M

Abstract

Vibrio alginolyticus caused great losses to aquaculture. Adhesion is an important virulence factor of V. alginolyticus. In this study, the relationship between V. alginolyticus adhesion and type II secretion system genes (secA, secD, secF, yajC, and yidC) was determined using gene silencing, qRT-PCR and in vitro adhesion assay. The results showed that the expression of target genes and the bacterial adhesion exhibited significant decreases after transient gene silencing and stable gene silencing, which indicated that secA, secD, secF, yajC, and yidC played roles in the bacterial adhesion of V. alginolyticus. The expression of secA, secD, secF, yajC, and yidC were significantly influenced by temperature, salinity, pH and starvation. The results indicated that the expression of secA, secD, secF, yajC, and yidC were sensitive to different environmental factors, whereas environmental factors can affect V. alginolyticus adhesion via the expression of secA, secD, secF, yajC, and yidC.

Authors+Show Affiliations

Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.State Key Laboratory of Large Yellow Croaker Breeding, Ningde, Fujian, China. College of Ocean & Earth Sciences, Xiamen University, Xiamen, Fujian, China.Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China. State Key Laboratory of Large Yellow Croaker Breeding, Ningde, Fujian, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29057613

Citation

Guo, Lina, et al. "SecA, secD, secF, yajC, and yidC Contribute to the Adhesion Regulation of Vibrio Alginolyticus." MicrobiologyOpen, vol. 7, no. 2, 2018, pp. e00551.
Guo L, Huang L, Su Y, et al. SecA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. Microbiologyopen. 2018;7(2):e00551.
Guo, L., Huang, L., Su, Y., Qin, Y., Zhao, L., & Yan, Q. (2018). SecA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. MicrobiologyOpen, 7(2), pp. e00551. doi:10.1002/mbo3.551.
Guo L, et al. SecA, secD, secF, yajC, and yidC Contribute to the Adhesion Regulation of Vibrio Alginolyticus. Microbiologyopen. 2018;7(2):e00551. PubMed PMID: 29057613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus. AU - Guo,Lina, AU - Huang,Lixing, AU - Su,Yongquan, AU - Qin,Yingxue, AU - Zhao,Lingmin, AU - Yan,Qingpi, Y1 - 2017/10/23/ PY - 2017/07/03/received PY - 2017/08/26/revised PY - 2017/09/18/accepted PY - 2017/10/24/pubmed PY - 2018/12/12/medline PY - 2017/10/24/entrez KW - Vibrio alginolyticus KW - secA KW - secD KW - secF KW - yajC KW - yidC KW - RNAi KW - adhesion KW - environmental factors SP - e00551 EP - e00551 JF - MicrobiologyOpen JO - Microbiologyopen VL - 7 IS - 2 N2 - Vibrio alginolyticus caused great losses to aquaculture. Adhesion is an important virulence factor of V. alginolyticus. In this study, the relationship between V. alginolyticus adhesion and type II secretion system genes (secA, secD, secF, yajC, and yidC) was determined using gene silencing, qRT-PCR and in vitro adhesion assay. The results showed that the expression of target genes and the bacterial adhesion exhibited significant decreases after transient gene silencing and stable gene silencing, which indicated that secA, secD, secF, yajC, and yidC played roles in the bacterial adhesion of V. alginolyticus. The expression of secA, secD, secF, yajC, and yidC were significantly influenced by temperature, salinity, pH and starvation. The results indicated that the expression of secA, secD, secF, yajC, and yidC were sensitive to different environmental factors, whereas environmental factors can affect V. alginolyticus adhesion via the expression of secA, secD, secF, yajC, and yidC. SN - 2045-8827 UR - https://www.unboundmedicine.com/medline/citation/29057613/secA_secD_secF_yajC_and_yidC_contribute_to_the_adhesion_regulation_of_Vibrio_alginolyticus_ L2 - https://doi.org/10.1002/mbo3.551 DB - PRIME DP - Unbound Medicine ER -