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The Operophtera brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage within Genus Alphabaculovirus.
Viruses 2017; 9(10)V

Abstract

Operophtera brumata nucleopolyhedrovirus (OpbuNPV) infects the larvae of the winter moth, Operophtera brumata. As part of an effort to explore the pesticidal potential of OpbuNPV, an isolate of this virus from Massachusetts (USA)-OpbuNPV-MA-was characterized by electron microscopy of OpbuNPV occlusion bodies (OBs) and by sequencing of the viral genome. The OBs of OpbuNPV-MA consisted of irregular polyhedra and contained virions consisting of a single rod-shaped nucleocapsid within each envelope. Presumptive cypovirus OBs were also detected in sections of the OB preparation. The OpbuNPV-MA genome assembly yielded a circular contig of 119,054 bp and was found to contain little genetic variation, with most polymorphisms occurring at a frequency of < 6%. A total of 130 open reading frames (ORFs) were annotated, including the 38 core genes of Baculoviridae, along with five homologous repeat (hr) regions. The results of BLASTp and phylogenetic analysis with selected ORFs indicated that OpbuNPV-MA is not closely related to other alphabaculoviruses. Phylogenies based on concatenated core gene amino acid sequence alignments placed OpbuNPV-MA on a basal branch lying outside other alphabaculovirus clades. These results indicate that OpbuNPV-MA represents a divergent baculovirus lineage that appeared early during the diversification of genus Alphabaculovirus.

Authors+Show Affiliations

Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA. Robert.L.Harrison@ars.usda.gov.Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA. Daniel.Rowley@ars.usda.gov.Electron and Confocal Microscopy Unit, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA. Joseph.Mowery@ars.usda.gov.Electron and Confocal Microscopy Unit, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA. Gary.Bauchan@ars.usda.gov.Department of Microbiology, University of Massachusetts-Amherst, Amherst, MA 01003, USA. jburand@microbio.umass.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29065456

Citation

Harrison, Robert L., et al. "The Operophtera Brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage Within Genus Alphabaculovirus." Viruses, vol. 9, no. 10, 2017.
Harrison RL, Rowley DL, Mowery JD, et al. The Operophtera brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage within Genus Alphabaculovirus. Viruses. 2017;9(10).
Harrison, R. L., Rowley, D. L., Mowery, J. D., Bauchan, G. R., & Burand, J. P. (2017). The Operophtera brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage within Genus Alphabaculovirus. Viruses, 9(10), doi:10.3390/v9100307.
Harrison RL, et al. The Operophtera Brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage Within Genus Alphabaculovirus. Viruses. 2017 10 21;9(10) PubMed PMID: 29065456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Operophtera brumata Nucleopolyhedrovirus (OpbuNPV) Represents an Early, Divergent Lineage within Genus Alphabaculovirus. AU - Harrison,Robert L, AU - Rowley,Daniel L, AU - Mowery,Joseph D, AU - Bauchan,Gary R, AU - Burand,John P, Y1 - 2017/10/21/ PY - 2017/09/18/received PY - 2017/10/12/revised PY - 2017/10/17/accepted PY - 2017/10/26/entrez PY - 2017/10/27/pubmed PY - 2018/6/1/medline KW - Alphabaculovirus KW - Operophtera brumata KW - baculovirus KW - cypovirus KW - genome KW - winter moth JF - Viruses JO - Viruses VL - 9 IS - 10 N2 - Operophtera brumata nucleopolyhedrovirus (OpbuNPV) infects the larvae of the winter moth, Operophtera brumata. As part of an effort to explore the pesticidal potential of OpbuNPV, an isolate of this virus from Massachusetts (USA)-OpbuNPV-MA-was characterized by electron microscopy of OpbuNPV occlusion bodies (OBs) and by sequencing of the viral genome. The OBs of OpbuNPV-MA consisted of irregular polyhedra and contained virions consisting of a single rod-shaped nucleocapsid within each envelope. Presumptive cypovirus OBs were also detected in sections of the OB preparation. The OpbuNPV-MA genome assembly yielded a circular contig of 119,054 bp and was found to contain little genetic variation, with most polymorphisms occurring at a frequency of < 6%. A total of 130 open reading frames (ORFs) were annotated, including the 38 core genes of Baculoviridae, along with five homologous repeat (hr) regions. The results of BLASTp and phylogenetic analysis with selected ORFs indicated that OpbuNPV-MA is not closely related to other alphabaculoviruses. Phylogenies based on concatenated core gene amino acid sequence alignments placed OpbuNPV-MA on a basal branch lying outside other alphabaculovirus clades. These results indicate that OpbuNPV-MA represents a divergent baculovirus lineage that appeared early during the diversification of genus Alphabaculovirus. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/29065456/The_Operophtera_brumata_Nucleopolyhedrovirus__OpbuNPV__Represents_an_Early_Divergent_Lineage_within_Genus_Alphabaculovirus_ L2 - http://www.mdpi.com/resolver?pii=v9100307 DB - PRIME DP - Unbound Medicine ER -