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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis from pooled rectal, pharyngeal and urine specimens in men who have sex with men.
Sex Transm Infect. 2018 06; 94(4):293-297.ST

Abstract

OBJECTIVES

Screening of men who have sex with men (MSM) for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) requires sampling from anorectal and pharyngeal sites in addition to urogenital sampling. Due to the cost of testing multiple anatomical sites individually testing of pooled specimens has potential merit. The Cepheid GeneXpert CT/NG assay (GeneXpert), which also has potential for point-of-care nucleic acid testing in the sexual health clinic, has not been assessed for pooled specimen testing.

METHODS

We prospectively compared GeneXpert testing of pooled pharyngeal and rectal swabs with urine samples to standard of care testing of individual specimens from 107 participants using the Roche cobas 4800 CT/NG assay (cobas) for CT and NG in high-risk MSM attending an inner city sexual health clinic.

RESULTS

We found testing of pooled pharyngeal, rectal and urine samples by the GeneXpert to have 100% agreement for NG and 94% overall agreement for CT when compared with individual specimen testing by cobas. For CT testing, 14 cases were detected for both tests, 4for cobas only, 2 for GeneXpert only and 89 participants were negative for both tests.

CONCLUSIONS

Pooled specimen CT and NG testing by the GeneXpert was accurate when compared with single specimen testing and has potential for screening MSM for CT and NG. The role of pooled specimen testing with the GeneXpert as a point-of-care nucleic acid test in MSM requires further investigation.

Authors+Show Affiliations

Department of Microbiology, PathWest Laboratory Medicine WA (LMWA), Queen Elizabeth II (QEII) Medical Centre, Nedlands, Western Australia, Australia. School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia, Australia.Department of Microbiology, PathWest Laboratory Medicine WA (LMWA), Queen Elizabeth II (QEII) Medical Centre, Nedlands, Western Australia, Australia.M Clinic, WA AIDS Council, West Perth, Western Australia, Australia.School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia, Australia. M Clinic, WA AIDS Council, West Perth, Western Australia, Australia.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29066627

Citation

Speers, David John, et al. "Detection of Neisseria Gonorrhoeae and Chlamydia Trachomatis From Pooled Rectal, Pharyngeal and Urine Specimens in Men Who Have Sex With Men." Sexually Transmitted Infections, vol. 94, no. 4, 2018, pp. 293-297.
Speers DJ, Chua IJ, Manuel J, et al. Detection of Neisseria gonorrhoeae and Chlamydia trachomatis from pooled rectal, pharyngeal and urine specimens in men who have sex with men. Sex Transm Infect. 2018;94(4):293-297.
Speers, D. J., Chua, I. J., Manuel, J., & Marshall, L. (2018). Detection of Neisseria gonorrhoeae and Chlamydia trachomatis from pooled rectal, pharyngeal and urine specimens in men who have sex with men. Sexually Transmitted Infections, 94(4), 293-297. https://doi.org/10.1136/sextrans-2017-053303
Speers DJ, et al. Detection of Neisseria Gonorrhoeae and Chlamydia Trachomatis From Pooled Rectal, Pharyngeal and Urine Specimens in Men Who Have Sex With Men. Sex Transm Infect. 2018;94(4):293-297. PubMed PMID: 29066627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Neisseria gonorrhoeae and Chlamydia trachomatis from pooled rectal, pharyngeal and urine specimens in men who have sex with men. AU - Speers,David John, AU - Chua,I-Ly Joanna, AU - Manuel,Justin, AU - Marshall,Lewis, Y1 - 2017/10/24/ PY - 2017/06/08/received PY - 2017/09/24/revised PY - 2017/10/08/accepted PY - 2017/10/27/pubmed PY - 2018/11/7/medline PY - 2017/10/26/entrez KW - chlamydiatrachomatis KW - gonorrhoea KW - men KW - molecular techniques KW - sexual health SP - 293 EP - 297 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 94 IS - 4 N2 - OBJECTIVES: Screening of men who have sex with men (MSM) for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) requires sampling from anorectal and pharyngeal sites in addition to urogenital sampling. Due to the cost of testing multiple anatomical sites individually testing of pooled specimens has potential merit. The Cepheid GeneXpert CT/NG assay (GeneXpert), which also has potential for point-of-care nucleic acid testing in the sexual health clinic, has not been assessed for pooled specimen testing. METHODS: We prospectively compared GeneXpert testing of pooled pharyngeal and rectal swabs with urine samples to standard of care testing of individual specimens from 107 participants using the Roche cobas 4800 CT/NG assay (cobas) for CT and NG in high-risk MSM attending an inner city sexual health clinic. RESULTS: We found testing of pooled pharyngeal, rectal and urine samples by the GeneXpert to have 100% agreement for NG and 94% overall agreement for CT when compared with individual specimen testing by cobas. For CT testing, 14 cases were detected for both tests, 4for cobas only, 2 for GeneXpert only and 89 participants were negative for both tests. CONCLUSIONS: Pooled specimen CT and NG testing by the GeneXpert was accurate when compared with single specimen testing and has potential for screening MSM for CT and NG. The role of pooled specimen testing with the GeneXpert as a point-of-care nucleic acid test in MSM requires further investigation. SN - 1472-3263 UR - https://www.unboundmedicine.com/medline/citation/29066627/Detection_of_Neisseria_gonorrhoeae_and_Chlamydia_trachomatis_from_pooled_rectal_pharyngeal_and_urine_specimens_in_men_who_have_sex_with_men_ L2 - https://sti.bmj.com/lookup/pmidlookup?view=long&pmid=29066627 DB - PRIME DP - Unbound Medicine ER -