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Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis.
JBRA Assist Reprod. 2017 12 01; 21(4):295-301.JA

Abstract

OBJECTIVE

To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis.

METHODS

A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist.

RESULTS

A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele. Apoptosis was not influenced by varicocele. Conventional semen parameters were significantly worse in individuals with the disease. On the other hand, in men with varicocele, Spearman's correlation demonstrated that early apoptosis and abnormal MMP showed a positive and significant correlation with sperm DNA fragmentation.

CONCLUSION

Men with varicocele had worse semen parameters, including increased levels of sperm DNA fragmentation, inactive mitochondria, and abnormal chromatin packaging. These changes are possible causes of infertility in individuals with varicocele.

Authors+Show Affiliations

Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil.Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil. Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29068181

Citation

Dieamant, Felipe, et al. "Semen Parameters in Men With Varicocele: DNA Fragmentation, Chromatin Packaging, Mitochondrial Membrane Potential, and Apoptosis." JBRA Assisted Reproduction, vol. 21, no. 4, 2017, pp. 295-301.
Dieamant F, Petersen CG, Mauri AL, et al. Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis. JBRA Assist Reprod. 2017;21(4):295-301.
Dieamant, F., Petersen, C. G., Mauri, A. L., Conmar, V., Mattila, M., Vagnini, L. D., Renzi, A., Costa, B. P., Zamara, C., Oliveira, J. B. A., Baruffi, R. L. R., & Franco, J. G. (2017). Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis. JBRA Assisted Reproduction, 21(4), 295-301. https://doi.org/10.5935/1518-0557.20170053
Dieamant F, et al. Semen Parameters in Men With Varicocele: DNA Fragmentation, Chromatin Packaging, Mitochondrial Membrane Potential, and Apoptosis. JBRA Assist Reprod. 2017 12 1;21(4):295-301. PubMed PMID: 29068181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis. AU - Dieamant,Felipe, AU - Petersen,Claudia G, AU - Mauri,Ana L, AU - Conmar,Vanessa, AU - Mattila,Mariana, AU - Vagnini,Laura D, AU - Renzi,Adriana, AU - Costa,Bruna P, AU - Zamara,Camila, AU - Oliveira,João Batista A, AU - Baruffi,Ricardo L R, AU - Franco,José G,Jr Y1 - 2017/12/01/ PY - 2017/10/27/pubmed PY - 2018/7/28/medline PY - 2017/10/26/entrez KW - DNA damage KW - DNA fragmentation KW - apoptosis KW - chromatin packaging KW - inactive mitochondria KW - varicocele SP - 295 EP - 301 JF - JBRA assisted reproduction JO - JBRA Assist Reprod VL - 21 IS - 4 N2 - OBJECTIVE: To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis. METHODS: A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist. RESULTS: A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele. Apoptosis was not influenced by varicocele. Conventional semen parameters were significantly worse in individuals with the disease. On the other hand, in men with varicocele, Spearman's correlation demonstrated that early apoptosis and abnormal MMP showed a positive and significant correlation with sperm DNA fragmentation. CONCLUSION: Men with varicocele had worse semen parameters, including increased levels of sperm DNA fragmentation, inactive mitochondria, and abnormal chromatin packaging. These changes are possible causes of infertility in individuals with varicocele. SN - 1518-0557 UR - https://www.unboundmedicine.com/medline/citation/29068181/Semen_parameters_in_men_with_varicocele:_DNA_fragmentation_chromatin_packaging_mitochondrial_membrane_potential_and_apoptosis_ L2 - https://doi.org/10.5935/1518-0557.20170053 DB - PRIME DP - Unbound Medicine ER -