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[Effect of Moxibustion and Electroacupuncture on Th 1/Th 2 Immune Balance in Rats with Experimental Colitis].
Zhen Ci Yan Jiu. 2016 Jun 25; 41(3):210-4.ZC

Abstract

OBJECTIVE

To observe the effect of moxibustion and electroacupuncture (EA) stimulation of "Tianshu" (ST 25) and "Qihai" (CV 6) on Th 1/Th 2 immune balance in rats with experimental ulcerative colitis (UC).

METHODS

The UC rats were randomly allocated to model group, moxibustion group and EA group (n=10 in each group). Another 10 rats were used as the control group. The UC model was established by injection of fresh colonic mucosal tissue-made antigen emulsifier (purified mucosal supernate plus complete Freund's adjuvant, 3.5 mg on day one, and 7 mg thereafter) into the bilateral toes, inguinal grooves, back, and the abdominal cavity on day 1, 10, 17 and 24, respectively. Medicinal paste (containing Cortex Cinnamomi,Flos Carthami, etc.)-separated moxibustion or EA (200 Hz, 2 mA) was applied to ST 25 and CV 6 for 20 min, once daily for 14 days. The mRNA expression levels of interferon-γ (INF-γ), IL-12, IL-4 and IL-10 in colon tissues were detected using quantitative RT-PCR and their content determined using enzyme linked immunosorbent assay (ELISA). The colon tissue cell ratios of CD 4+ INF-γ+ and CD 4+ IL-4+ were analyzed by flow cytometry.

RESULTS

Compared with the control group, the mRNA and contents of colonic INF-γ and IL-12 and the ratio of CD 4+ INF-γ+ and CD 4+ IL-4+ were significantly higher in the model group (P<0.05), and the IL-4 and IL-10 mRNA expression and contents considerably lower after UC modeling (P<0.05). Following medicinal paste-separated moxibustion and EA interventions, the increased INF-γ and IL-12 mRNA and contents and the increased ratio of CD 4+ INF-γ+ and CD 4+ IL-4+, and the decreased IL-4 and IL-10 mRNA and contents were reversed (P<0.05). No significant differences were found between the moxibustion and EA groups in the levels of all the indexes (P>0.05).

CONCLUSIONS

Both medicinal paste-separated moxibustion and EA interventions may regulate the balance between Th 1 and Th 2 cells in the colonic tissues of UC rats, suggesting an improvement of the immune function by down-regulating INF-γ and IL-12 levels and up-regulating IL-4 and IL-10 levels.

Authors+Show Affiliations

Department of Rehabilitation, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471000, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29071907

Citation

Chen, Yan-Ping. "[Effect of Moxibustion and Electroacupuncture On Th 1/Th 2 Immune Balance in Rats With Experimental Colitis]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 41, no. 3, 2016, pp. 210-4.
Chen YP. [Effect of Moxibustion and Electroacupuncture on Th 1/Th 2 Immune Balance in Rats with Experimental Colitis]. Zhen Ci Yan Jiu. 2016;41(3):210-4.
Chen, Y. P. (2016). [Effect of Moxibustion and Electroacupuncture on Th 1/Th 2 Immune Balance in Rats with Experimental Colitis]. Zhen Ci Yan Jiu = Acupuncture Research, 41(3), 210-4.
Chen YP. [Effect of Moxibustion and Electroacupuncture On Th 1/Th 2 Immune Balance in Rats With Experimental Colitis]. Zhen Ci Yan Jiu. 2016 Jun 25;41(3):210-4. PubMed PMID: 29071907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Moxibustion and Electroacupuncture on Th 1/Th 2 Immune Balance in Rats with Experimental Colitis]. A1 - Chen,Yan-Ping, PY - 2017/10/27/entrez PY - 2016/6/25/pubmed PY - 2018/7/14/medline SP - 210 EP - 4 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 41 IS - 3 N2 - OBJECTIVE: To observe the effect of moxibustion and electroacupuncture (EA) stimulation of "Tianshu" (ST 25) and "Qihai" (CV 6) on Th 1/Th 2 immune balance in rats with experimental ulcerative colitis (UC). METHODS: The UC rats were randomly allocated to model group, moxibustion group and EA group (n=10 in each group). Another 10 rats were used as the control group. The UC model was established by injection of fresh colonic mucosal tissue-made antigen emulsifier (purified mucosal supernate plus complete Freund's adjuvant, 3.5 mg on day one, and 7 mg thereafter) into the bilateral toes, inguinal grooves, back, and the abdominal cavity on day 1, 10, 17 and 24, respectively. Medicinal paste (containing Cortex Cinnamomi,Flos Carthami, etc.)-separated moxibustion or EA (200 Hz, 2 mA) was applied to ST 25 and CV 6 for 20 min, once daily for 14 days. The mRNA expression levels of interferon-γ (INF-γ), IL-12, IL-4 and IL-10 in colon tissues were detected using quantitative RT-PCR and their content determined using enzyme linked immunosorbent assay (ELISA). The colon tissue cell ratios of CD 4+ INF-γ+ and CD 4+ IL-4+ were analyzed by flow cytometry. RESULTS: Compared with the control group, the mRNA and contents of colonic INF-γ and IL-12 and the ratio of CD 4+ INF-γ+ and CD 4+ IL-4+ were significantly higher in the model group (P<0.05), and the IL-4 and IL-10 mRNA expression and contents considerably lower after UC modeling (P<0.05). Following medicinal paste-separated moxibustion and EA interventions, the increased INF-γ and IL-12 mRNA and contents and the increased ratio of CD 4+ INF-γ+ and CD 4+ IL-4+, and the decreased IL-4 and IL-10 mRNA and contents were reversed (P<0.05). No significant differences were found between the moxibustion and EA groups in the levels of all the indexes (P>0.05). CONCLUSIONS: Both medicinal paste-separated moxibustion and EA interventions may regulate the balance between Th 1 and Th 2 cells in the colonic tissues of UC rats, suggesting an improvement of the immune function by down-regulating INF-γ and IL-12 levels and up-regulating IL-4 and IL-10 levels. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29071907/[Effect_of_Moxibustion_and_Electroacupuncture_on_Th_1/Th_2_Immune_Balance_in_Rats_with_Experimental_Colitis]_ L2 - https://medlineplus.gov/ulcerativecolitis.html DB - PRIME DP - Unbound Medicine ER -