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[Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain].
Zhen Ci Yan Jiu. 2016 Jun 25; 41(3):225-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) intervention on pain thresholds (PT) and contents of β-endorphin (EP) in the hypothalamus and spinal cord, and the expression of 5-HT in the dorsal raphe nucleus(DRN)in rats with pelmatic incisional pain, so as to investigate the underlying mechanisms of acupuncture in reducing post-operative pain.

METHODS

Wistar rats were randomized into normal control, model, EA and non-acupoint groups (n=8/group). The pelmatic pain model was induced by making an incision (about 1 cm in length, to the fascia and muscle layers) from the heel towards the toes. EA (2 Hz, 1.5-2 V) was applied to "Zusanli" (ST 36) and "Kunlun" (BL 60) or non-acupoint (about 3 mm beside the ST 36 and BL 60) on the affected side for 20 min, once daily for three days. The thermal PT and mechanical PT were measured before and after operation and after EA. The contents of β-EP in hypothalamus and L3-S4 spinal cord were detected using enzyme linked immunosorbent assay (ELISA) and the expressions of β-EP in hypothalamus and 5-HT in DRN were measured with immunohistochemistry.

RESULTS

After EA intervention, the markedly decreased mechanical and thermal pain thresholds on day 1 and 3 after paw incision were significantly increased in the EA group (P<0.05), but not in the non-acupoint group (P>0.05). The hypothalamic β-EP content was significantly higher in the model group than in the normal group (P<0.05), and further up-regulated in the EA group (not the non-acupoint group) than in the model group (P<0.05). In addition, the hypothalamic β-EP immunoreactive (IR)-positive cell number and 5-HT immunoactivity level in DRN were also considerably up-regulated in the EA group (P<0.05) but not in the non-acupoint group (P>0.05). No significant changes were found in the lumbar spinal β-EP contents in the model, EA and non-acupoint groups (P>0.05).

CONCLUSIONS

EA stimulation of "Zusanli"(ST 36) and "Kunlun" (BL 60) has an analgesic effect in pelmatic incision pain rats, which may be related to its effects in raising the level of hypothalamic β-EP and the expression of 5-HT in DRN.

Authors+Show Affiliations

Laboratory of Anesthesiology, Weifang Medical College, Weifang 261053, China.Laboratory of Anesthesiology, Weifang Medical College, Weifang 261053, China.Laboratory of Anesthesiology, Weifang Medical College, Weifang 261053, China.Laboratory of Anesthesiology, Weifang Medical College, Weifang 261053, China. wgz@wfmc.edu.cn.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29071910

Citation

Jia, Wang-Hua, et al. "[Effect of Electroacupuncture Intervention On Β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats With Pelmatic Incisional Pain]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 41, no. 3, 2016, pp. 225-9.
Jia WH, Chi WY, Lou C, et al. [Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain]. Zhen Ci Yan Jiu. 2016;41(3):225-9.
Jia, W. H., Chi, W. Y., Lou, C., & Wang, G. Z. (2016). [Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain]. Zhen Ci Yan Jiu = Acupuncture Research, 41(3), 225-9.
Jia WH, et al. [Effect of Electroacupuncture Intervention On Β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats With Pelmatic Incisional Pain]. Zhen Ci Yan Jiu. 2016 Jun 25;41(3):225-9. PubMed PMID: 29071910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain]. AU - Jia,Wang-Hua, AU - Chi,Wen-Ying, AU - Lou,Chao, AU - Wang,Gui-Zhi, PY - 2017/10/27/entrez PY - 2016/6/25/pubmed PY - 2018/7/14/medline SP - 225 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 41 IS - 3 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) intervention on pain thresholds (PT) and contents of β-endorphin (EP) in the hypothalamus and spinal cord, and the expression of 5-HT in the dorsal raphe nucleus(DRN)in rats with pelmatic incisional pain, so as to investigate the underlying mechanisms of acupuncture in reducing post-operative pain. METHODS: Wistar rats were randomized into normal control, model, EA and non-acupoint groups (n=8/group). The pelmatic pain model was induced by making an incision (about 1 cm in length, to the fascia and muscle layers) from the heel towards the toes. EA (2 Hz, 1.5-2 V) was applied to "Zusanli" (ST 36) and "Kunlun" (BL 60) or non-acupoint (about 3 mm beside the ST 36 and BL 60) on the affected side for 20 min, once daily for three days. The thermal PT and mechanical PT were measured before and after operation and after EA. The contents of β-EP in hypothalamus and L3-S4 spinal cord were detected using enzyme linked immunosorbent assay (ELISA) and the expressions of β-EP in hypothalamus and 5-HT in DRN were measured with immunohistochemistry. RESULTS: After EA intervention, the markedly decreased mechanical and thermal pain thresholds on day 1 and 3 after paw incision were significantly increased in the EA group (P<0.05), but not in the non-acupoint group (P>0.05). The hypothalamic β-EP content was significantly higher in the model group than in the normal group (P<0.05), and further up-regulated in the EA group (not the non-acupoint group) than in the model group (P<0.05). In addition, the hypothalamic β-EP immunoreactive (IR)-positive cell number and 5-HT immunoactivity level in DRN were also considerably up-regulated in the EA group (P<0.05) but not in the non-acupoint group (P>0.05). No significant changes were found in the lumbar spinal β-EP contents in the model, EA and non-acupoint groups (P>0.05). CONCLUSIONS: EA stimulation of "Zusanli"(ST 36) and "Kunlun" (BL 60) has an analgesic effect in pelmatic incision pain rats, which may be related to its effects in raising the level of hypothalamic β-EP and the expression of 5-HT in DRN. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29071910/[Effect_of_Electroacupuncture_Intervention_on_β_endorphin_Levels_of_Hypothalamus_and_Spinal_Cord_Tissues_in_Rats_with_Pelmatic_Incisional_Pain]_ DB - PRIME DP - Unbound Medicine ER -