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[Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats].
Zhen Ci Yan Jiu 2017; 42(2):141-4ZC

Abstract

OBJECTIVE

To observe the effect of acupoint injection on eosinophils (EOS) counts and expression of eotaxin in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improving AR.

METHODS

Twenty-four Sprague-Dawley (SD) rats were randomly divided into normal, model and acupoint injection groups (n=8 in each group). The AR model was established by intraperitoneal injection of ovalbumin sensitization. Bilateral "Yingxiang"(LI 20) and "Yintang"(GV 29) were selected for acupoint injection of the mixture solution of lidocaine, dexamethasone, and transfer factor (0.1 mL/acupoint) on the 1st, 5th, 9th, and 13th day after AR model established, a total of four times. EOS in the nasal mucosa was counted under light microscope after HE staining. Protein and mRNA expressions of eotaxin in the nasal mucosa were detected by immunohistochemical and RT-PCR methods, respectively.

RESULTS

Compared with the normal group, EOS counts, protein and mRNA expressions of eotaxin in the nasal mucosa were significantly higher in the model group (P<0.05). Compared with the model group, EOS counts, protein and mRNA expressions of eotaxin in the nasal mucosa were significantly lower in the acupoint injection group (P<0.05).

CONCLUSIONS

Acupoint injection can reduce the nasal mucosa inflammation by suppressing the protein and mRNA expressions of eotaxin, decreasing the infiltration and gathering of EOS in the nasal mucosa.

Authors+Show Affiliations

Clinical Medical College of Guizhou Medical University, Guiyang 550004, China.The Affliated Hospital of Guizhou Medical University, Guiyang 550004.The Affliated Hospital of Guizhou Medical University, Guiyang 550004. gzlilihong@163.com.Clinical Medical College of Guizhou Medical University, Guiyang 550004, China.Clinical Medical College of Guizhou Medical University, Guiyang 550004, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29071962

Citation

Zhang, Yuan, et al. "[Effect of Acupoint Injection On Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 2, 2017, pp. 141-4.
Zhang Y, Hou XR, Li LH, et al. [Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu. 2017;42(2):141-4.
Zhang, Y., Hou, X. R., Li, L. H., Yang, M., & Liang, F. H. (2017). [Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 42(2), pp. 141-4.
Zhang Y, et al. [Effect of Acupoint Injection On Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu. 2017 Apr 25;42(2):141-4. PubMed PMID: 29071962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Acupoint Injection on Eosinophil Counts,Protein and mRNA Expressions of Eotaxin in Nasal Mucosa of Allergic Rhinitis Rats]. AU - Zhang,Yuan, AU - Hou,Xun-Rui, AU - Li,Li-Hong, AU - Yang,Meng, AU - Liang,Fei-Hong, PY - 2017/10/27/entrez PY - 2017/10/27/pubmed PY - 2018/1/13/medline SP - 141 EP - 4 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 2 N2 - OBJECTIVE: To observe the effect of acupoint injection on eosinophils (EOS) counts and expression of eotaxin in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improving AR. METHODS: Twenty-four Sprague-Dawley (SD) rats were randomly divided into normal, model and acupoint injection groups (n=8 in each group). The AR model was established by intraperitoneal injection of ovalbumin sensitization. Bilateral "Yingxiang"(LI 20) and "Yintang"(GV 29) were selected for acupoint injection of the mixture solution of lidocaine, dexamethasone, and transfer factor (0.1 mL/acupoint) on the 1st, 5th, 9th, and 13th day after AR model established, a total of four times. EOS in the nasal mucosa was counted under light microscope after HE staining. Protein and mRNA expressions of eotaxin in the nasal mucosa were detected by immunohistochemical and RT-PCR methods, respectively. RESULTS: Compared with the normal group, EOS counts, protein and mRNA expressions of eotaxin in the nasal mucosa were significantly higher in the model group (P<0.05). Compared with the model group, EOS counts, protein and mRNA expressions of eotaxin in the nasal mucosa were significantly lower in the acupoint injection group (P<0.05). CONCLUSIONS: Acupoint injection can reduce the nasal mucosa inflammation by suppressing the protein and mRNA expressions of eotaxin, decreasing the infiltration and gathering of EOS in the nasal mucosa. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29071962/[Effect_of_Acupoint_Injection_on_Eosinophil_CountsProtein_and_mRNA_Expressions_of_Eotaxin_in_Nasal_Mucosa_of_Allergic_Rhinitis_Rats]_ L2 - http://www.diseaseinfosearch.org/result/7665 DB - PRIME DP - Unbound Medicine ER -