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[Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats].
Zhen Ci Yan Jiu. 2017 Jun 25; 42(3):240-5.ZC

Abstract

OBJECTIVE

To observe the effect of point-moxibustion and electroacupuncture(EA) on gastrointestinal motility, and expression of endothelial nitric oxide synthase(eNOS) mRNA and angiotensin 2(AT Ⅱ) mRNA in gastric antrum in diabetic gastroparesis(DGP) rats, so as to reveal the different effect between point-moxibustion and EA, and explore the mechanism underlying improvement of DGP.

METHODS

Forty SD rats were randomly divided into normal, model, point-moxibustion and EA groups, 10 rats in each group. The DGP model was established by intraperitoneal injection of streptozotocin (STZ, 2%, 55 mmol/kg) and fed with high-sugar and high-fat fodder. "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Liangmen" (ST 21) were chosen to be stimulated with moxibustion at 0, 10 and 20 min in the point-moxibustion group or with EA for 20 min in the EA group. The treatment was conducted once daily for 15 days. After treatment, blood glucose, gastric emptying rate and small intestinal propulsive rate were measured. The plasma endothelin 1(ET-1) content was detected by ELISA. The mRNA expression of eNOS and AT Ⅱ in gastric antrum were detected by Real-time PCR.

RESULTS

Compared with the normal group, the blood glucose in the model group was significantly higher (P<0.01). Compared with the model group, the blood glucose decreased significantly in the point-moxibustion group and EA group (P<0.05). In comparison with the normal group, gastric emptying rate and small intestinal propulsive rate were decreased, the content of plasma ET-1 was increased, the expression of eNOS mRNA in gastric antrum was significantly reduced and the expression of ATⅡ mRNA was significantly increased in the model group(P<0.01). In comparison with the model group, gastric emptying rate and small intestinal propulsive rate were increased, the content of plasma ET-1 was decreased, the expression of eNOS mRNA in gastric antrum was higher and the expression of AT Ⅱ mRNA was significantly lower in both point-moxibustion group and EA group (P<0.05), and there were no significant differences between the two treatment groups in above mentioned indexes (P>0.05).

CONCLUSIONS

Both point-moxibustion and EA can effectively promote gastrointestinal motility in DGP rats and improve the symptoms of delayed gastric emptying. The two therapies are effective, and their mechanism may be related to the increase of eNOS mRNA expression and the decrease of AT Ⅱ mRNA expression in gastric antrum.

Authors+Show Affiliations

College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China. 624755064@qq.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29071981

Citation

Liu, Li, et al. "[Effect of Point-moxibustion and Electroacupuncture On the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 3, 2017, pp. 240-5.
Liu L, Wu XF, Zheng XN, et al. [Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2017;42(3):240-5.
Liu, L., Wu, X. F., Zheng, X. N., Guo, X., Yue, Z. H., Liu, M., Peng, Y., & Yuan, J. L. (2017). [Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 42(3), 240-5.
Liu L, et al. [Effect of Point-moxibustion and Electroacupuncture On the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2017 Jun 25;42(3):240-5. PubMed PMID: 29071981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Point-moxibustion and Electroacupuncture on the Expression of Endothelial Nitric Oxide Synthase mRNA and Angiotensin 2 mRNA in Gastric Antrum in Diabetic Gastroparesis Rats]. AU - Liu,Li, AU - Wu,Xue-Fen, AU - Zheng,Xue-Na, AU - Guo,Xin, AU - Yue,Zeng-Hui, AU - Liu,Mi, AU - Peng,Yan, AU - Yuan,Jian-Ling, PY - 2017/10/27/entrez PY - 2017/10/27/pubmed PY - 2018/7/17/medline SP - 240 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 3 N2 - OBJECTIVE: To observe the effect of point-moxibustion and electroacupuncture(EA) on gastrointestinal motility, and expression of endothelial nitric oxide synthase(eNOS) mRNA and angiotensin 2(AT Ⅱ) mRNA in gastric antrum in diabetic gastroparesis(DGP) rats, so as to reveal the different effect between point-moxibustion and EA, and explore the mechanism underlying improvement of DGP. METHODS: Forty SD rats were randomly divided into normal, model, point-moxibustion and EA groups, 10 rats in each group. The DGP model was established by intraperitoneal injection of streptozotocin (STZ, 2%, 55 mmol/kg) and fed with high-sugar and high-fat fodder. "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Liangmen" (ST 21) were chosen to be stimulated with moxibustion at 0, 10 and 20 min in the point-moxibustion group or with EA for 20 min in the EA group. The treatment was conducted once daily for 15 days. After treatment, blood glucose, gastric emptying rate and small intestinal propulsive rate were measured. The plasma endothelin 1(ET-1) content was detected by ELISA. The mRNA expression of eNOS and AT Ⅱ in gastric antrum were detected by Real-time PCR. RESULTS: Compared with the normal group, the blood glucose in the model group was significantly higher (P<0.01). Compared with the model group, the blood glucose decreased significantly in the point-moxibustion group and EA group (P<0.05). In comparison with the normal group, gastric emptying rate and small intestinal propulsive rate were decreased, the content of plasma ET-1 was increased, the expression of eNOS mRNA in gastric antrum was significantly reduced and the expression of ATⅡ mRNA was significantly increased in the model group(P<0.01). In comparison with the model group, gastric emptying rate and small intestinal propulsive rate were increased, the content of plasma ET-1 was decreased, the expression of eNOS mRNA in gastric antrum was higher and the expression of AT Ⅱ mRNA was significantly lower in both point-moxibustion group and EA group (P<0.05), and there were no significant differences between the two treatment groups in above mentioned indexes (P>0.05). CONCLUSIONS: Both point-moxibustion and EA can effectively promote gastrointestinal motility in DGP rats and improve the symptoms of delayed gastric emptying. The two therapies are effective, and their mechanism may be related to the increase of eNOS mRNA expression and the decrease of AT Ⅱ mRNA expression in gastric antrum. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29071981/[Effect_of_Point_moxibustion_and_Electroacupuncture_on_the_Expression_of_Endothelial_Nitric_Oxide_Synthase_mRNA_and_Angiotensin_2_mRNA_in_Gastric_Antrum_in_Diabetic_Gastroparesis_Rats]_ DB - PRIME DP - Unbound Medicine ER -