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[Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats].
Zhen Ci Yan Jiu. 2017 Feb 25; 42(1):20-4.ZC

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) stimulation with different electrical current intensities and therapeutic intervals on learning-memory ability and expression of β-amyloid peptide Aβ 1-40 and arginine vasopressin (AVP) genes in the hippocampal CA 1 region in vascular dementia (VD) rats, so as to provide evidence for treatment of VD.

METHODS

A total of 48 male SD rats were randomly divided into sham, model, 0.5 mA-5 d-EA, 1.5 mA-5 d-EA, 0.5 mA-1 d-EA and 1.5 mA-1 d-EA groups (n=8 in each group). The VD model was established using modified 4-vessels occlusion method. EA (alternative 2 Hz/15 Hz, 0.5 mA, 1.5 mA) was applied to "Baihui" (GV 20) and "Dazhui" (GV 14) for 30 min, once a day (1 d) or once every 5 d for 10 times. The learning-memory ability was detected using Morris water maze tests (place navigation task and spatial probe trials), and the expression of Aβ 1-40 and AVP genes in hippocampal CA 1 region was determined using real time-PCR.

RESULTS

In comparison with the sham group, the average escape latency of place navigation task, and the duration for crossing the target-platform for the 1st time (spatial navigation task) were significantly increased (P<0.05), and the times to cross the target-platform within 2 min (spatial probe trials) were significantly decreased in the model group (P<0.05), suggesting a reduction of learning-memory ability. The expression level of Aβ 1-40 mRNA was remarkably increased (P<0.05), and that of AVP mRNA notably decreased in the model group (P<0.05). Following EA intervention, the increased escape latency and the duration of crossing the target-platform for the 1st time and the increased Aβ 1-40 mRNA expression, and the decreased target-platform crossing times within 2 min as well the down-regulated AVP mRNA expression level were reversed in the 4 EA groups compared with the model group (P<0.05). The therapeutic effects of higher stimulating intensity (1.5 mA-1 d-EA and 1.5 mA-5 d-EA) were markedly superior to those of lower intensity (0.5 mA-1 d-EA and 0.5 mA-5 d-EA), and the effects of higher frequency of EA intervention (0.5 mA-5 d-EA and 1.5 mA-5 d-EA) were obviously superior to those of lower frequency of EA (0.5 mA-1 d-EA and 1.5 mA-1 d-EA) in suppressing the increased 3 indexes and the decreased 2 indexes mentioned above (P<0.05).

CONCLUSIONS

EA can improve the learning-memory ability of VD rats, which Feb be related to its effects in inhibiting the expression of Aβ 1-40 mRNA and up-regulating the expression of AVP mRNA in hippocampal CA 1 region; and the therapeutic effects of higher stimulating intensity and higher intervention frequency are obviously better than those of lower stimulating intensity and lower therapeutic frequency.

Authors+Show Affiliations

Clinical Medical College of Acu-moxibustion Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.Clinical Medical College of Acu-moxibustion Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.Clinical Medical College of Acu-moxibustion Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.Clinical Medical College of Acu-moxibustion Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. shaoying@gzucm.edu.cn.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29071993

Citation

Jiang, Ling-Ge, et al. "[Influence of Electroacupuncture Stimulation With Different Intensities and Therapeutic Intervals On Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 1, 2017, pp. 20-4.
Jiang LG, Zhang HW, Zhang Z, et al. [Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats]. Zhen Ci Yan Jiu. 2017;42(1):20-4.
Jiang, L. G., Zhang, H. W., Zhang, Z., & Shao, Y. (2017). [Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 42(1), 20-4.
Jiang LG, et al. [Influence of Electroacupuncture Stimulation With Different Intensities and Therapeutic Intervals On Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats]. Zhen Ci Yan Jiu. 2017 Feb 25;42(1):20-4. PubMed PMID: 29071993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ 1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats]. AU - Jiang,Ling-Ge, AU - Zhang,Hua-Wei, AU - Zhang,Zheng, AU - Shao,Ying, PY - 2017/10/27/entrez PY - 2017/10/27/pubmed PY - 2018/1/13/medline SP - 20 EP - 4 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 1 N2 - OBJECTIVE: To observe the influence of electroacupuncture (EA) stimulation with different electrical current intensities and therapeutic intervals on learning-memory ability and expression of β-amyloid peptide Aβ 1-40 and arginine vasopressin (AVP) genes in the hippocampal CA 1 region in vascular dementia (VD) rats, so as to provide evidence for treatment of VD. METHODS: A total of 48 male SD rats were randomly divided into sham, model, 0.5 mA-5 d-EA, 1.5 mA-5 d-EA, 0.5 mA-1 d-EA and 1.5 mA-1 d-EA groups (n=8 in each group). The VD model was established using modified 4-vessels occlusion method. EA (alternative 2 Hz/15 Hz, 0.5 mA, 1.5 mA) was applied to "Baihui" (GV 20) and "Dazhui" (GV 14) for 30 min, once a day (1 d) or once every 5 d for 10 times. The learning-memory ability was detected using Morris water maze tests (place navigation task and spatial probe trials), and the expression of Aβ 1-40 and AVP genes in hippocampal CA 1 region was determined using real time-PCR. RESULTS: In comparison with the sham group, the average escape latency of place navigation task, and the duration for crossing the target-platform for the 1st time (spatial navigation task) were significantly increased (P<0.05), and the times to cross the target-platform within 2 min (spatial probe trials) were significantly decreased in the model group (P<0.05), suggesting a reduction of learning-memory ability. The expression level of Aβ 1-40 mRNA was remarkably increased (P<0.05), and that of AVP mRNA notably decreased in the model group (P<0.05). Following EA intervention, the increased escape latency and the duration of crossing the target-platform for the 1st time and the increased Aβ 1-40 mRNA expression, and the decreased target-platform crossing times within 2 min as well the down-regulated AVP mRNA expression level were reversed in the 4 EA groups compared with the model group (P<0.05). The therapeutic effects of higher stimulating intensity (1.5 mA-1 d-EA and 1.5 mA-5 d-EA) were markedly superior to those of lower intensity (0.5 mA-1 d-EA and 0.5 mA-5 d-EA), and the effects of higher frequency of EA intervention (0.5 mA-5 d-EA and 1.5 mA-5 d-EA) were obviously superior to those of lower frequency of EA (0.5 mA-1 d-EA and 1.5 mA-1 d-EA) in suppressing the increased 3 indexes and the decreased 2 indexes mentioned above (P<0.05). CONCLUSIONS: EA can improve the learning-memory ability of VD rats, which Feb be related to its effects in inhibiting the expression of Aβ 1-40 mRNA and up-regulating the expression of AVP mRNA in hippocampal CA 1 region; and the therapeutic effects of higher stimulating intensity and higher intervention frequency are obviously better than those of lower stimulating intensity and lower therapeutic frequency. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29071993/[Influence_of_Electroacupuncture_Stimulation_with_Different_Intensities_and_Therapeutic_Intervals_on_Learning_memory_Ability_and_Expression_of_Aβ_1_40_and_Arginine_Vasopressin_Genes_in_the_Hippocampal_CA_1_Region_in_VD_Rats]_ DB - PRIME DP - Unbound Medicine ER -