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[Effects of Electroacupuncture on Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats with Neck Incision Pain].
Zhen Ci Yan Jiu. 2017 Aug 25; 42(4):283-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation of "Futu"(LI 18), etc. on activities of satellite glial cells (SGCs) in the dorsal root ganglia (DRG) in rats with neck-incision pain so as to explore its mechanism underlying reduction of post-surgical pain of thyroidectomy.

METHODS

Male SD rats were randomly divided into control, model, EA-Futu (LI 18), EA-Hegu (LI 4)-Neiguan (PC 6), and EA-Zusanli (ST 36)-Yanglingquan (GB 34) groups, with 20 rats in each group. The neck-incision pain model was established by making a longitudinal incision and repeated mechanical stimulation. In the EA-LI 18, EA-LI 4-PC 6 and EA-ST 36-GB 34 groups, EA stimulation was administrated for 30 min, once a day,continuously for 3 days. The thermal pain threshold (PT) of the neck-incision region was detected. The immunoactivity of glial fibrillary acidic protein (GFAP,a specific marker for SGCs) and connexin 43 (Cx 43) of DRGs (C 2-C 6) was determined by fluorescent immunohistochemistry. The expression levels of GFAP, IL-1 β, IL-6, and TNF-α mRNAs were determined by quantitative Real-time PCR, and the contents of IL-1 β,IL-6,TNF-α assayed by enzyme linked immunosorbent assay (ELISA) and the expression of Cx 43 protein was detected by Western blot.

RESULTS

After EA intervention at LI 18 and LI 4-PC 6 (but not ST 36-GB 34), neck incision-induced reduction of the thermal PT was obviously prolonged in comparison with the model group (P<0.05),suggesting a pain relief. The expression levels of GFAP, IL-1 β, IL-6 and TNF-α mRNAs and Cx 43 protein, and the contents of IL-1 β, IL-6 and TNF-α in C 2-C 6 DRGs were all significantly up-regulated in the model group relevant to those of the control group (P<0.05). Following EA, modeling induced dramatic increase of expression of GFAP, IL-1 β, IL-6 and TNF-α mRNAs and Cx 43 protein in both EA-LI 18 and EA-LI 4-PC 6 groups, and the contents of IL-1 β and TNF-α in the EA-LI 18 group, IL-6 in the EA-LI 4-PC 6 group was considerably down-regulated (P<0.05). In comparison with the model group, no significant changes were found in all the abovementioned indexes of EA-ST 36 -GB 34 group except the down-regulated IL-1 β and TNF-α mRNAs, in the contents of IL-1 β and TNF-α of the EA-LI 4-PC 6 group, and in the IL-6 content of the EA-LI 18 group (P>0.05).

CONCLUSIONS

EA stimulation of LI 18 and LI 4-PC 6 can significantly suppress pain reaction of neck incision in the rat, which is closely associated with its effects in down-regulating the activity of SGCs, decreasing the release of pro-inflammatory cytokines and in weakening the expression of Cx 43 in the cervical DRGs.

Authors+Show Affiliations

Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; Institute of Acupuncture and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; Institute of Acupuncture and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117.School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. yysh1950@sina.com.Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. 13521898023@163.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29072007

Citation

Qiao, Li-Na, et al. "[Effects of Electroacupuncture On Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats With Neck Incision Pain]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 42, no. 4, 2017, pp. 283-9.
Qiao LN, Tan LH, Yang JJ, et al. [Effects of Electroacupuncture on Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats with Neck Incision Pain]. Zhen Ci Yan Jiu. 2017;42(4):283-9.
Qiao, L. N., Tan, L. H., Yang, J. J., Gao, Q. L., Zhu, J., Rong, P. J., Zhu, B., Yang, Y. S., & Liu, J. L. (2017). [Effects of Electroacupuncture on Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats with Neck Incision Pain]. Zhen Ci Yan Jiu = Acupuncture Research, 42(4), 283-9.
Qiao LN, et al. [Effects of Electroacupuncture On Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats With Neck Incision Pain]. Zhen Ci Yan Jiu. 2017 Aug 25;42(4):283-9. PubMed PMID: 29072007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Electroacupuncture on Activities of Satellite Glial Cells of Dorsal Root Ganglia in Rats with Neck Incision Pain]. AU - Qiao,Li-Na, AU - Tan,Lian-Hong, AU - Yang,Jiao-Jiao, AU - Gao,Qiao-Ling, AU - Zhu,Jiang, AU - Rong,Pei-Jing, AU - Zhu,Bing, AU - Yang,Yong-Sheng, AU - Liu,Jun-Ling, PY - 2017/10/27/entrez PY - 2017/10/27/pubmed PY - 2018/7/17/medline SP - 283 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 42 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of "Futu"(LI 18), etc. on activities of satellite glial cells (SGCs) in the dorsal root ganglia (DRG) in rats with neck-incision pain so as to explore its mechanism underlying reduction of post-surgical pain of thyroidectomy. METHODS: Male SD rats were randomly divided into control, model, EA-Futu (LI 18), EA-Hegu (LI 4)-Neiguan (PC 6), and EA-Zusanli (ST 36)-Yanglingquan (GB 34) groups, with 20 rats in each group. The neck-incision pain model was established by making a longitudinal incision and repeated mechanical stimulation. In the EA-LI 18, EA-LI 4-PC 6 and EA-ST 36-GB 34 groups, EA stimulation was administrated for 30 min, once a day,continuously for 3 days. The thermal pain threshold (PT) of the neck-incision region was detected. The immunoactivity of glial fibrillary acidic protein (GFAP,a specific marker for SGCs) and connexin 43 (Cx 43) of DRGs (C 2-C 6) was determined by fluorescent immunohistochemistry. The expression levels of GFAP, IL-1 β, IL-6, and TNF-α mRNAs were determined by quantitative Real-time PCR, and the contents of IL-1 β,IL-6,TNF-α assayed by enzyme linked immunosorbent assay (ELISA) and the expression of Cx 43 protein was detected by Western blot. RESULTS: After EA intervention at LI 18 and LI 4-PC 6 (but not ST 36-GB 34), neck incision-induced reduction of the thermal PT was obviously prolonged in comparison with the model group (P<0.05),suggesting a pain relief. The expression levels of GFAP, IL-1 β, IL-6 and TNF-α mRNAs and Cx 43 protein, and the contents of IL-1 β, IL-6 and TNF-α in C 2-C 6 DRGs were all significantly up-regulated in the model group relevant to those of the control group (P<0.05). Following EA, modeling induced dramatic increase of expression of GFAP, IL-1 β, IL-6 and TNF-α mRNAs and Cx 43 protein in both EA-LI 18 and EA-LI 4-PC 6 groups, and the contents of IL-1 β and TNF-α in the EA-LI 18 group, IL-6 in the EA-LI 4-PC 6 group was considerably down-regulated (P<0.05). In comparison with the model group, no significant changes were found in all the abovementioned indexes of EA-ST 36 -GB 34 group except the down-regulated IL-1 β and TNF-α mRNAs, in the contents of IL-1 β and TNF-α of the EA-LI 4-PC 6 group, and in the IL-6 content of the EA-LI 18 group (P>0.05). CONCLUSIONS: EA stimulation of LI 18 and LI 4-PC 6 can significantly suppress pain reaction of neck incision in the rat, which is closely associated with its effects in down-regulating the activity of SGCs, decreasing the release of pro-inflammatory cytokines and in weakening the expression of Cx 43 in the cervical DRGs. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29072007/[Effects_of_Electroacupuncture_on_Activities_of_Satellite_Glial_Cells_of_Dorsal_Root_Ganglia_in_Rats_with_Neck_Incision_Pain]_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -