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Xiaochaihutang attenuates liver fibrosis by activation of Nrf2 pathway in rats.
Biomed Pharmacother. 2017 Dec; 96:847-853.BP

Abstract

Xiaochaihutang (XCHT) is a decoction of seven botanical extracts used for liver diseases traditionally in East Asia. However, few studies have investigated its anti-hepatic fibrosis effects and the mechanisms of action. Oxidative stress is one of the key factors responsible for occurrence and development of hepatic fibrosis and nuclear factor erythroid 2-related factor 2 (Nrf2) serves as a major regulator of the cellular defense system against oxidative stress. The aim of the present study was to investigate the effect of XCHT on liver fibrosis and focuse on the Nrf2 pathway in the protection of XCHT against CCl4-induced oxidative stress. Liver fibrosis was induced by repeated injection of CCl4 over a period of 9 weeks. Starting from the 6th week, rats in treatment groups were given the appropriate doses of XCHT granules and Silybin. We discovered that CCl4 caused significant fibrosis damage in rat liver, and XCHT (5g/kg and 10g/kg, po for 4 weeks) significantly improved the liver functions and fibrosis degree. XCHT treatment significantly decreased the number of α-SMA positive stained cells, indicating suppression of activated hepatic stellate cells (HSCs). Compared with the CCl4 treatment, administration of XCHT significantly increased the hepatic levels of Nqo1, HO-1, GCLC and GCLM, the major components of the Nrf2 pathway. These studies demonstrated that XCHT is an effective therapeutic agent for treatment of hepatic fibrosis and the mechanism of action might be due to up-regulation of the Nrf2 pathway against oxidative stress, making further inhibition of activated HSCs.

Authors+Show Affiliations

Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China. Electronic address: lijin010502@163.com.Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, Guizhou, 563003, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29078262

Citation

Li, Jin, et al. "Xiaochaihutang Attenuates Liver Fibrosis By Activation of Nrf2 Pathway in Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 96, 2017, pp. 847-853.
Li J, Hu R, Xu S, et al. Xiaochaihutang attenuates liver fibrosis by activation of Nrf2 pathway in rats. Biomed Pharmacother. 2017;96:847-853.
Li, J., Hu, R., Xu, S., Li, Y., Qin, Y., Wu, Q., & Xiao, Z. (2017). Xiaochaihutang attenuates liver fibrosis by activation of Nrf2 pathway in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 96, 847-853. https://doi.org/10.1016/j.biopha.2017.10.065
Li J, et al. Xiaochaihutang Attenuates Liver Fibrosis By Activation of Nrf2 Pathway in Rats. Biomed Pharmacother. 2017;96:847-853. PubMed PMID: 29078262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xiaochaihutang attenuates liver fibrosis by activation of Nrf2 pathway in rats. AU - Li,Jin, AU - Hu,Rui, AU - Xu,Shangfu, AU - Li,Yuanyang, AU - Qin,Ying, AU - Wu,Qin, AU - Xiao,Zhi, Y1 - 2017/11/06/ PY - 2017/09/06/received PY - 2017/10/09/revised PY - 2017/10/14/accepted PY - 2017/10/29/pubmed PY - 2018/7/26/medline PY - 2017/10/29/entrez KW - Hepatic fibrosis KW - Nrf2 pathway KW - Oxidative stress KW - Xiaochaihutang SP - 847 EP - 853 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 96 N2 - Xiaochaihutang (XCHT) is a decoction of seven botanical extracts used for liver diseases traditionally in East Asia. However, few studies have investigated its anti-hepatic fibrosis effects and the mechanisms of action. Oxidative stress is one of the key factors responsible for occurrence and development of hepatic fibrosis and nuclear factor erythroid 2-related factor 2 (Nrf2) serves as a major regulator of the cellular defense system against oxidative stress. The aim of the present study was to investigate the effect of XCHT on liver fibrosis and focuse on the Nrf2 pathway in the protection of XCHT against CCl4-induced oxidative stress. Liver fibrosis was induced by repeated injection of CCl4 over a period of 9 weeks. Starting from the 6th week, rats in treatment groups were given the appropriate doses of XCHT granules and Silybin. We discovered that CCl4 caused significant fibrosis damage in rat liver, and XCHT (5g/kg and 10g/kg, po for 4 weeks) significantly improved the liver functions and fibrosis degree. XCHT treatment significantly decreased the number of α-SMA positive stained cells, indicating suppression of activated hepatic stellate cells (HSCs). Compared with the CCl4 treatment, administration of XCHT significantly increased the hepatic levels of Nqo1, HO-1, GCLC and GCLM, the major components of the Nrf2 pathway. These studies demonstrated that XCHT is an effective therapeutic agent for treatment of hepatic fibrosis and the mechanism of action might be due to up-regulation of the Nrf2 pathway against oxidative stress, making further inhibition of activated HSCs. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/29078262/Xiaochaihutang_attenuates_liver_fibrosis_by_activation_of_Nrf2_pathway_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)34613-9 DB - PRIME DP - Unbound Medicine ER -