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Analgesic effects and pharmacologic mechanisms of the Gelsemium alkaloid koumine on a rat model of postoperative pain.
Sci Rep. 2017 10 27; 7(1):14269.SR

Abstract

Postoperative pain (POP) of various durations is a common complication of surgical procedures. POP is caused by nerve damage and inflammatory responses that are difficult to treat. The neuroinflammation-glia-steroid network is known to be important in POP. It has been reported that the Gelsemium alkaloid koumine possesses analgesic, anti-inflammatory and neurosteroid modulating activities. This study was undertaken to test the analgesic effects of koumine against POP and explore the underlying pharmacologic mechanisms. Our results showed that microglia and astroglia were activated in the spinal dorsal horn post-incision, along with an increase of proinflammatory cytokines (interleukin 1β, interleukin 6, and tumor necrosis factor α). Both subcutaneous and intrathecal (i.t.) koumine treatment after incision significantly prevented mechanical allodynia and thermal hyperalgesia, inhibited microglial and astroglial activation, and suppressed expression of proinflammatory cytokines. Moreover, the analgesic effects of koumine were antagonized by i.t. administration of translocator protein (18 kDa) (TSPO) antagonist PK11195 and GABAA receptor antagonist bicuculline. Together, koumine prevented mechanical allodynia and thermal hyperalgesia caused by POP. The pharmacologic mechanism of koumine-mediated analgesia might involve inhibition of spinal neuroinflammation and activation of TSPO. These data suggested that koumine might be a potential pharmacotherapy for the management of POP.

Authors+Show Affiliations

Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China. changxiyu@mail.fjmu.edu.cn. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, 350122, Fujian, People's Republic of China. changxiyu@mail.fjmu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29079733

Citation

Xiong, Bo-Jun, et al. "Analgesic Effects and Pharmacologic Mechanisms of the Gelsemium Alkaloid Koumine On a Rat Model of Postoperative Pain." Scientific Reports, vol. 7, no. 1, 2017, p. 14269.
Xiong BJ, Xu Y, Jin GL, et al. Analgesic effects and pharmacologic mechanisms of the Gelsemium alkaloid koumine on a rat model of postoperative pain. Sci Rep. 2017;7(1):14269.
Xiong, B. J., Xu, Y., Jin, G. L., Liu, M., Yang, J., & Yu, C. X. (2017). Analgesic effects and pharmacologic mechanisms of the Gelsemium alkaloid koumine on a rat model of postoperative pain. Scientific Reports, 7(1), 14269. https://doi.org/10.1038/s41598-017-14714-0
Xiong BJ, et al. Analgesic Effects and Pharmacologic Mechanisms of the Gelsemium Alkaloid Koumine On a Rat Model of Postoperative Pain. Sci Rep. 2017 10 27;7(1):14269. PubMed PMID: 29079733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analgesic effects and pharmacologic mechanisms of the Gelsemium alkaloid koumine on a rat model of postoperative pain. AU - Xiong,Bo-Jun, AU - Xu,Ying, AU - Jin,Gui-Lin, AU - Liu,Ming, AU - Yang,Jian, AU - Yu,Chang-Xi, Y1 - 2017/10/27/ PY - 2017/06/08/received PY - 2017/10/13/accepted PY - 2017/10/29/entrez PY - 2017/10/29/pubmed PY - 2019/7/17/medline SP - 14269 EP - 14269 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Postoperative pain (POP) of various durations is a common complication of surgical procedures. POP is caused by nerve damage and inflammatory responses that are difficult to treat. The neuroinflammation-glia-steroid network is known to be important in POP. It has been reported that the Gelsemium alkaloid koumine possesses analgesic, anti-inflammatory and neurosteroid modulating activities. This study was undertaken to test the analgesic effects of koumine against POP and explore the underlying pharmacologic mechanisms. Our results showed that microglia and astroglia were activated in the spinal dorsal horn post-incision, along with an increase of proinflammatory cytokines (interleukin 1β, interleukin 6, and tumor necrosis factor α). Both subcutaneous and intrathecal (i.t.) koumine treatment after incision significantly prevented mechanical allodynia and thermal hyperalgesia, inhibited microglial and astroglial activation, and suppressed expression of proinflammatory cytokines. Moreover, the analgesic effects of koumine were antagonized by i.t. administration of translocator protein (18 kDa) (TSPO) antagonist PK11195 and GABAA receptor antagonist bicuculline. Together, koumine prevented mechanical allodynia and thermal hyperalgesia caused by POP. The pharmacologic mechanism of koumine-mediated analgesia might involve inhibition of spinal neuroinflammation and activation of TSPO. These data suggested that koumine might be a potential pharmacotherapy for the management of POP. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29079733/Analgesic_effects_and_pharmacologic_mechanisms_of_the_Gelsemium_alkaloid_koumine_on_a_rat_model_of_postoperative_pain_ L2 - https://doi.org/10.1038/s41598-017-14714-0 DB - PRIME DP - Unbound Medicine ER -