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Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass.
J Exp Biol 2017; 220(Pt 24):4720-4732JE

Abstract

Spatiotemporal changes in branchial ionocyte distribution were investigated following transfer from seawater (SW) to freshwater (FW) in Japanese seabass. The mRNA expression levels of cystic fibrosis transmembrane conductance regulator (CFTR) and Na+/K+/2Cl- cotransporter 1a (NKCC1a) in the gills rapidly decreased after transfer to FW, whereas Na+/H+ exchanger 3 (NHE3) and Na+/Cl- cotransporter 2 (NCC2) expression were upregulated following the transfer. Using quadruple-color whole-mount immunofluorescence staining with anti-Na+/K+-ATPase, anti-NHE3, anti-CFTR and T4 (anti-NKCC1a/NCC2) antibodies, we classified ionocytes into one SW type and two FW types: NHE3 cell and NCC2 cell. Time course observation after transfer revealed an intermediate type between SW-type and FW-type NHE3 ionocytes, suggesting functional plasticity of ionocytes. Finally, on the basis of the ionocyte classification of Japanese seabass, we observed the location of ionocyte subtypes on frozen sections of the gill filaments stained by triple-color immunofluorescence staining. Our observation indicated that SW-type ionocytes transformed into FW-type NHE3 ionocytes and at the same time shifted their distribution from filaments to lamellae. However, FW-specific NCC2 ionocytes appeared mainly in the filaments. Taken together, these findings indicate that ionocytes originated from undifferentiated cells in the filaments and expanded their distribution to the lamellae during FW acclimation.

Authors+Show Affiliations

Department of Life Sciences, Toyo University, Itakura, Gunma 374-0193, Japan inokuchi@toyo.jp.National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Imabari, Ehime 794-2305, Japan.Faculty of Agriculture, University of Miyazaki, Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan.Department of Anatomy, St Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan.Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29084852

Citation

Inokuchi, Mayu, et al. "Functional Classification of Gill Ionocytes and Spatiotemporal Changes in Their Distribution After Transfer From Seawater to Freshwater in Japanese Seabass." The Journal of Experimental Biology, vol. 220, no. Pt 24, 2017, pp. 4720-4732.
Inokuchi M, Nakamura M, Miyanishi H, et al. Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass. J Exp Biol. 2017;220(Pt 24):4720-4732.
Inokuchi, M., Nakamura, M., Miyanishi, H., Hiroi, J., & Kaneko, T. (2017). Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass. The Journal of Experimental Biology, 220(Pt 24), pp. 4720-4732. doi:10.1242/jeb.167320.
Inokuchi M, et al. Functional Classification of Gill Ionocytes and Spatiotemporal Changes in Their Distribution After Transfer From Seawater to Freshwater in Japanese Seabass. J Exp Biol. 2017 Dec 15;220(Pt 24):4720-4732. PubMed PMID: 29084852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass. AU - Inokuchi,Mayu, AU - Nakamura,Masahiro, AU - Miyanishi,Hiroshi, AU - Hiroi,Junya, AU - Kaneko,Toyoji, Y1 - 2017/10/30/ PY - 2017/07/25/received PY - 2017/10/24/accepted PY - 2017/11/1/pubmed PY - 2018/3/27/medline PY - 2017/11/1/entrez KW - Filament KW - Gill KW - Ionocyte KW - Japanese seabass KW - Lamella KW - Osmoregulation SP - 4720 EP - 4732 JF - The Journal of experimental biology JO - J. Exp. Biol. VL - 220 IS - Pt 24 N2 - Spatiotemporal changes in branchial ionocyte distribution were investigated following transfer from seawater (SW) to freshwater (FW) in Japanese seabass. The mRNA expression levels of cystic fibrosis transmembrane conductance regulator (CFTR) and Na+/K+/2Cl- cotransporter 1a (NKCC1a) in the gills rapidly decreased after transfer to FW, whereas Na+/H+ exchanger 3 (NHE3) and Na+/Cl- cotransporter 2 (NCC2) expression were upregulated following the transfer. Using quadruple-color whole-mount immunofluorescence staining with anti-Na+/K+-ATPase, anti-NHE3, anti-CFTR and T4 (anti-NKCC1a/NCC2) antibodies, we classified ionocytes into one SW type and two FW types: NHE3 cell and NCC2 cell. Time course observation after transfer revealed an intermediate type between SW-type and FW-type NHE3 ionocytes, suggesting functional plasticity of ionocytes. Finally, on the basis of the ionocyte classification of Japanese seabass, we observed the location of ionocyte subtypes on frozen sections of the gill filaments stained by triple-color immunofluorescence staining. Our observation indicated that SW-type ionocytes transformed into FW-type NHE3 ionocytes and at the same time shifted their distribution from filaments to lamellae. However, FW-specific NCC2 ionocytes appeared mainly in the filaments. Taken together, these findings indicate that ionocytes originated from undifferentiated cells in the filaments and expanded their distribution to the lamellae during FW acclimation. SN - 1477-9145 UR - https://www.unboundmedicine.com/medline/citation/29084852/Functional_classification_of_gill_ionocytes_and_spatiotemporal_changes_in_their_distribution_after_transfer_from_seawater_to_freshwater_in_Japanese_seabass_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=29084852 DB - PRIME DP - Unbound Medicine ER -