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Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae.
Molecules. 2017 Oct 31; 22(11)M

Abstract

In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions-CPP1, CPP2, and CPP3, (CPPs)-were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries.

Authors+Show Affiliations

College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China. xiaoliwang325@126.com.College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China. zhangyifeiyc@126.com.College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China. liuzhikaiyc@126.com.College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China. zhaomingqin@126.com.College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China. liupengfei523@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29088064

Citation

Wang, Xiaoli, et al. "Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated From Cortex Periplocae." Molecules (Basel, Switzerland), vol. 22, no. 11, 2017.
Wang X, Zhang Y, Liu Z, et al. Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae. Molecules. 2017;22(11).
Wang, X., Zhang, Y., Liu, Z., Zhao, M., & Liu, P. (2017). Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae. Molecules (Basel, Switzerland), 22(11). https://doi.org/10.3390/molecules22111866
Wang X, et al. Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated From Cortex Periplocae. Molecules. 2017 Oct 31;22(11) PubMed PMID: 29088064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae. AU - Wang,Xiaoli, AU - Zhang,Yifei, AU - Liu,Zhikai, AU - Zhao,Mingqin, AU - Liu,Pengfei, Y1 - 2017/10/31/ PY - 2017/10/13/received PY - 2017/10/27/revised PY - 2017/10/30/accepted PY - 2017/11/1/entrez PY - 2017/11/1/pubmed PY - 2018/6/9/medline KW - Cortex Periplocae KW - antioxidant activity KW - chemical composition KW - polysaccharides KW - purification JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 11 N2 - In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions-CPP1, CPP2, and CPP3, (CPPs)-were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/29088064/Purification_Characterization_and_Antioxidant_Activity_of_Polysaccharides_Isolated_from_Cortex_Periplocae_ L2 - https://www.mdpi.com/resolver?pii=molecules22111866 DB - PRIME DP - Unbound Medicine ER -